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Gel Casting Reagents

Description
Application
Product #
Acrylamide : N,N'-Methylenebisacrylamide 32.5:1 BioChemika, for electrophoresis 01703
Acrylamide/bis-acrylamide, 40% solution for electrophoresis, 29:1 These acrylamide/bis-acrylamide solutions are available at the most common ratios for use in protein and nucleic acid electrophoresis. The concentration is based on the total weight of both the acrylamide and bis-acrylamide.
 A7802
Acrylamide:N,N'-Methylenebisacrylamide 19:1 solution BioChemika, for molecular biology, mixture of acrylamide and BIS 19:1, 40% in H2O 01693
Acrylamide:N,N'-Methylenebisacrylamide 19:1 BioChemika, for electrophoresis 01705
Acrylamide:N,N'-Methylenebisacrylamide 19:1 BioChemika, for molecular biology 01707
Acrylamide:N,N'-Methylenebisacrylamide 24:1 solution BioChemika, for electrophoresis, mixture of acrylamide and BIS 24:1, 30% in H2O 01706
Acrylamide:N,N'-Methylenebisacrylamide 24:1 BioChemika, for electrophoresis 01704
Acrylamide:N,N'-Methylenebisacrylamide 29:1 solution BioChemika, for electrophoresis, mixture of acrylamide and BIS 29:1, 40% in H2O 01708
Acrylamide:N,N'-Methylenebisacrylamide 29:1 BioChemika, for molecular biology 01702
Acrylamide:N,N'-Methylenebisacrylamide 37.5:1 solution BioChemika, for electrophoresis, mixture of acrylamide and BIS 37.5:1, 40% in H2O 01709
Acrylamide:N,N'-Methylenebisacrylamide 37.5:1 BioChemika, for molecular biology 01701
Electrophoresis Film for Polyacrylamide Gels 7 mil (0.2 mm) thickness Electrophoresis film is a flexible, transparent polyester film specifically designed to support polyacrylamide gels. Ideal for casting horizontal IEF gels. One side has been treated with a hydrophilic coating which enhances adhesion with aqueous polyacrylamide gel media. The acrylamide monomers covalently attach to the coating during polymerization. Gels remain attached during electrophoresis, staining and drying.
 E0389
IGEPAL® CA-630 BioChemika 56741
IGEPAL® CA-630 for electrophoresis, suitable for 2-D electrophoresis Used in denaturing (urea) IEF and two-dimensional electrophoresis.1 Helps facilitate extrusion of the IEF capillary gels from the tubes.2 Used extensively with immobilized pH gradient (IPG) gels.3 Concentrations between 2-4% (v/v) are typically used in an IEF gel.
 I7771
Triton® X-100 for electrophoresis Detergent used for isoelectric focusing (IEF) and two-dimensional electrophoresis. Concentrations between 1-4% (v/v) are typically used in an IEF gel. Also used for cell permeabilization and protein solubilization including high molecular mass proteins.
 T8532
Urea for electrophoresis A chaotropic agent used to solubilize and denature proteins for denaturing isoelectric focusing and two-dimensional electrophoresis.
Used for the denaturation of proteins and as a mild solubilization agent for insoluble or denatured proteins. Useful for renaturing proteins from samples already denatured with 6 M guanidine chloride such as inclusion bodies. May be used with guanidine hydrochloride and dithiothreitrol (DTT) in the refolding of denatured proteins into their native or active form.
 U6504

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