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Other Stains for Electrophoresis

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Description	Application	Product #
ATX Ponceau S red staining solution BioChemika, concentrate for 250 ml (6 bottles)		09276
ATX Ponceau S red staining solution BioChemika, ready-to-use solution		09189
Alcian Blue 8GX certified by the Biological Stain Commission	Certified by the BSC for use in staining in Mowry's pH 2.5 method, in Scott's pH 5.7 method, and in Kreyberg's method for keratin and mucin.	A3157
Alcian Blue 8GX for electrophoresis, suitable for detection of glycoproteins		A9186
Amido Black Staining Solution 2X electrophoresis reagent	Naphthol Blue Black stain is designed for rapid staining of protein bands on nitrocellulose membranes (Western blots).¹ Blots are stained for 1 min and then destained for 30 minutes in 25% (v/v) isopropanol, 10% (v/v) acetic acid. Used to detect proteins in the lower microgram range with a clear background. Naphthol Blue Black can also be used for staining PAGE gels.²	A8181
Copper stain kit BioChemika		61182
Eosin Y disodium salt BioChemika, for electrophoresis		45235
Fast Green FCF Dye content ~90 %	Fast Green FCF dye is used for protein staining in IEF, native PAGE and SDS-PAGE. Fast Green staining is linear over a wider range of protein concentrations than Brilliant Blue R. After electrophoresis, fixing the proteins in the gel is recommended for maximum sensitivity. The gel can then be stained for 2 hr with 0.1% Fast Green FCF in either 30% (v/v) ethanol, 10% (v/v) acetic acid or in 7% acetic acid. The gel can be destained with either 30% (v/v) ethanol, 10% (v/v) acetic acid, or 7% acetic acid. The stained gel can be scanned at 625 nm. The detection sensitivity is approx. 30% that with Brilliant Blue R.	F7252
Fluorescamine >97% (TLC), powder	Non-fluorescent reagent that reacts readily under mild conditions with primary amines in amino acids and peptides to form stable, highly fluorescent compounds.^1,² Low background due to hydrolysis. Useful for the fluorometric assay of amino acids, protein, and proteolytic enzymes.³ Effectively blocks newly generated amino termini in protein sequence analyses.	F9015
GlycoProfile^™ III, Fluorescent Glycoprotein Detection Kit	Allows for the specific, sensitive fluorescent detection of the glycoproteins directly in gels. This kit can also be used to detect glycoproteins after transfer to PVDF membranes.	PP0300
Glycoprotein Detection Kit	The Glycoprotein Detection Kit is designed for the selective staining of glycoproteins on polyacrylamide gels and membranes using a modification of the Periodic Acid-Schiff (PAS) method.¹ Staining of sugar moieties of glycoproteins yields magenta bands with a colorless background. The periodic acid/Schiff reagent stains vicinal diol groups found mainly on peripheral sugars and sialic acids and is used as a general glycoprotein stain.²	GLYCOPRO
LUCY^® 565 solution BioChemika	Lucy 565 is a fluorescent stain for protein electrophoresis, with high sensitivity and easy, fast and robust staining procedure for all kinds of SDS gels. Lucy 565 is also suitable for neutral staining (e.g. for subsequent western blotting). Protein staining by Lucy 565 does not interfere with subsequent MALDI-MS. Sensitivity of routine procedure is ca. 5 ng/band, with linear response between 5 and 4000 ng/band	43772
LUCY^® 569 solution BioChemika	Lucy 569 is a fluorescent stain for protein electrophoresis, with good sensitivity and easy, fast and robust staining procedure for all kinds of SDS gels. Lucy 569 provides a broad linear dynamic range for protein quantification in electrophoresis. Protein staining by Lucy 569 does not interfere with subsequent MALDI-MS. Sensitivity of routine procedure is ca. 5 ng/band, with linear response between 5 and 6000 ng/band	41629
Naphthol Blue Black for electrophoresis	Naphthol blue black dye can be used to stain proteins on polyacrylamide gels, agarose gels and nitrocellulose membranes. After electrophoresis, fixing the proteins in the gel is recommended. The gel can then be stained with 0.1% Naphthol Blue Black in 7% (v/v) acetic acid for at least 2 hr and destained with a soluion of 7% (v/v) acetic acid. Detection sensitivity is approx. 20% that of Coomassie Blue R.	N3393
Oil Red O for electrophoresis	Oil Red O stain is suitable as a lipid/lipoprotein stain on cellulose acetate. A 0.2% stock solution is prepared in methanol. The stock solution (35 ml) is diluted with 10 mL of 1 M NaOH immediately before staining. The dye begins precipitating during staining. After staining (1 hr), the membrane is destained in methanol:glycerol (1:3). Often cited for use with agarose	O9755
Periodic acid for electrophoresis, ≥99%	Periodic acid is commonly used as a component for glycoprotein stains¹. Selective staining of glycoproteins on SDS-PAGE and membranes is carried out using a modification of the Periodic Acid-Schiff (PAS) method. Staining of sugar moieties of glycoproteins yields magenta bands with a colorless background. The Periodic Acid/Schiff Reagent stains vicinal diol groups found mainly on peripheral sugars and sialic acids and is used as a general glycoprotein stain². Periodic acid is also used to cleave reversible cross-linkers (e.g. DATD, DHEBA) in PAGE gels.	P0430
Ponceau S solution 0.1 % (w/v) in 5% acetic acid	Ponceau S stain comes ready-to-use and is designed for rapid (5 min) staining of protein bands on nitrocellulose or PVDF membranes (Western blots) and also for staining protein on cellulose acetate membranes. Ponceau S staining on nitrocellulose and PVDF is reversible by washing the stained membrane with 0.1 M NaOH for 1 min. The stained and destained membrane can then be immunologically detected (Western blotting). A product information sheet is supplied which includes the staining procedure for cellulose acetate, nitrocellulose, and PVDF.	P7170
Reversible Protein Detection Kit for Membranes and Polyacrylamide Gels	R-PROB is a unique protein detection system designed for staining of proteins on nylon, nitrocellulose, and PVDF membranes or PAGE gels with the detection sensitivity similar to that of Coomassie stains. Most other stains produce high backgrounds on nylon membranes due to strong charge interactions with the membrane. The mixing of equal amounts of Solution A and B activates the stain, which produces lavender stained protein bands. The staining is easily reversible with an EDTA solution so that the blot can be reused for Western blotting or for amino acid sequence analysis (PVDF). R-PROB does not contain the toxic chemicals cacodylic acid or potassium ferrocyanide.	RPROB
SYPRO^® Orange Protein Gel Stain		S5692
SYPRO^® Red Protein Gel Stain		S5817
SYPRO^® Ruby Protein Blot Stain		S4817
SYPRO^® Ruby Protein Gel Stain		S4942
SYPRO^® Tangerine Protein Gel Stain		S5942
Stains-All ~95%	Stains-all is suitable for differential staining of nucleic acids and proteins as follows; RNA (bluish purple), DNA (blue) and proteins (red). As little as 3 ng (123 BP fragment) of pBR322/Hae III digest DNA and 90 ng tRNA can be detected on a polyacrylamide gel. PAGE gels are stained in the dark and then destaining by removing the gel from the staining solution and exposing it to the light from a light box until sufficient destaining has occurred. A staining solution is typically made by dissolving the dye in formamide and buffer.	E9379

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