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    Search term:"Endotoxin-Free Plasmid"

    26 matches found for Endotoxin-Free Plasmid

    DNA & RNA Purification GenElute Endotoxin-free Plasmid Kits

    Description: Endotoxins (also known as lipopolysaccharides or LPS) are often co-purified with plasmid DNA and can significantly reduce transfection efficiencies in endotoxin-sensitive cell lines. The GenElute™ Endotoxin-free Plasmid Kits offer a simple, rapid, cost-effective method for purifying pla...

    DNA & RNA Purification: HP Endotoxin-Free Plasmid Maxiprep Kit

    Description: The GenElute™ HP Endotoxin-Free Plasmid Maxiprep Kit offers a novel and efficient method of isolating endotoxin-free plasmid DNA in 40 minutes. GenElute HP Endotoxin-Free Plasmid Kits include a filter syringe for clearing lysates and binding columns that are used with a vacuum format. •...

    DNA & RNA Purification Endotoxin-Free Megaprep Kit

    Description: The GenElute™ HP Endotoxin-Free Plasmid Megaprep Kit offers a simple, rapid, cost-effective method of isolating endotoxin-free plasmid DNA in 90 minutes or less. GenElute HP Endotoxin-Free Plasmid Kits include a filter for clearing lysates and binding columns that are used with a vacuum...

    July 2000 GenElute Kit

    A TECHNICAL APPLICATION NEWSLETTER VOL 1 July 2000 Molecular Biology Application Notes The GenEluteTM Endotoxin-free DNA Purification Kit: Pure DNA for Endotoxin-Sensitive Applications by Fuqiang Chen, Scott Weber and Jo Anne Kerschner Sigma-Aldrich Corporation, St. Louis, MO, USA Introduction Endot...

    DNA & RNA Purification HP Select Plasmid Gigaprep Kit

    Description | Procedure | Application | Beneficial Features | Order Information | Data Description: The GenElute™ HP Select Plasmid Gigaprep Kit offers a simple, rapid, and cost-effective method for isolating endotoxin-free plasmid DNA from recombinant E. coli cultures. Up to 15 mg of high copy plas...

    Life Science Quarterly Spring 2005

    A TECHNICAL APPLICATION NEWSLETTER VOL 6 ISSUE 1 Spring 2005 Editorial Board Managing Editor Rafael Valdes-Camin Editor Sean Battles Design Firm Northbank Communications Ltd. The Type House, Inc. Editorial Review Board Keith Jolliff, Ph.D. Christina Shasserre Keith Watling, Ph.D. Molecular Biology F...

    DNA & RNA Purification Kits for Plasmid Purification

    Purify with Sigma Sigma® Life Science delivers unrivaled quality, expertise and reliability. The GenElute™ portfolio of products includes a variety of kits and reagents for all your nucleic acid purification needs. Whether you are purifying DNA from blood or extracting RNA from fungi, Sigma can help...

    GenElute HP Endotoxin-Free Maxiprep Kit Article

    By Dave Cutter and Goodarz Goodarzi Sigma-Aldrich Corporation, St. Louis, MO, USA View pdf version (209 KB) On this page: • Introduction • Materials and Methods • Results and Discussion • Conclusions • Acknowledgements • References • Ordering Information Introduction Plasmid DNA purification kits th...

    Reagents for Functional Genomics Screening

    We provide the full spectrum of products needed to perform your screen. From cell lines to oligos, and everywhere in between, we have you covered. Search the product list below. Product No. Description D6171 Dulbecco’s Modified Eagle’s Medium - high glucose D8537 Dulbecco’s Phosphate Buffered Saline...

    Transfection Best Practices

    Click here to return to the Molecular Biology Guide The work of scientific research is an art which requires good attention to detail. The quality of transfection is highest when these commonly used best practices and notes on optimization are employed. • Cells should be healthy, actively dividing, ...

    Plasmid Purification Kits in Molecular Biology

    Purify with Sigma GenElute™ plasmid kits provide reliable isolation of high quality plasmid DNA. These kits are: • Designed with advanced silica bind and elute technology • Compatible with both vacuum or spin format • Formulated without phenol/chloroform These products allow researchers to maximize ...

    LS Innovations Archive

    Your gateway to Life Science products and services. The perfect complement to Life Science Innovations. Quick Links Functional Genomics/RNAi Custom Genomics and Proteomics Cell Biology Proteomics & Protein Expression Cell Culture Biochemicals & Reagents Online Product Catalog Current Issue Download ...

    Plasmid DNA purification kits by Sigma Aldrich

    Extraction of macromolecules such as DNA, RNA, and protein is one of the basic methods used in molecular biology. The process of extraction and purification of nucleic acids has evolved from being a complex, prolonged, and labor-intensive procedure. Nucleic acid purification technologies now give hi...

    Plasmid DNA Purification Overview

    Click here to return to the Molecular Biology Guide. Extraction of macromolecules such as DNA, RNA, and protein is one of the basic methods used in molecular biology. The process of extraction and purification of nucleic acids has evolved from being a complex, prolonged, and labor-intensive procedur...

    PCR Technologies Additional Resources

    • Introduction and Historical Timelines • Polymerase Chain Reaction • Quantitative PCR • Digital PCR • Quantitative PCR and Digital PCR Detection Methods • PCR/qPCR/dPCR Assay Design • Sample Purification and Quality Assessment • Reverse Transcription • Assay Optimization and Validation • Data Analy...

    shRNA Frequently Asked Questions for Functional Genomics and RNAi

    What should I do if I have trouble obtaining high yields of plasmid DNA? Generally, high yields of DNA can be achieved with much of the collection. However, viral-based vectors are known to be somewhat difficult to prep. If particular clones are troublesome, we recommend streaking the bacterial stoc...

    Technical Articles on DNA and RNA Purification

    • GenElute™ HP Plasmid Kits • GenElute™ Bacterial Genomic DNA Miniprep Kit • GenElute™ Blood Genomic DNA Miniprep Kit • GenElute™ Mammalian Genomic DNA Miniprep Kit • GenElute™ Plant Genomic DNA Miniprep Kit • GenElute™ Gel Extract Kit • GenElute™ PCR Clean-Up Kit • GenElute™ Mammalian Total RNA Kit...

    shRNA Frequently Asked Questions for Functional Genomics and RNAi

    What should I do if I have trouble obtaining high yields of plasmid DNA? Generally, high yields of DNA can be achieved with much of the collection; however, viral-based vectors are known to be somewhat difficult to prep. If particular clones are troublesome, we recommend streaking the bacterial stoc...

    Troubleshooting - MISSION shRNA FAQ

    What should I do if I have trouble obtaining high yields of plasmid DNA? Generally, high yields of DNA can be achieved with much of the collection; however, viral-based vectors are known to be somewhat difficult to prep. If particular clones are troublesome, we recommend streaking the bacterial stoc...

    Tips for Cell Engineering using Cas9-GFP CRISPR plasmids

    Related Article Introduction to CRISPR A CRISPR/Cas-GFP Vector for Rapid Expression Verification View CRISPR Webinar CRISPR endonucleases have shown wide variation in their activity, even among multiple CRISPRs designed within close genomic proximity.1 For this reason, we highly recommend that you t...

    Formats — Glycerol FAQs

    What should I do if I have trouble obtaining high yields of plasmid DNA? How should I store my bacterial cultures? What is the expected pDNA yield from glycerol stocks? I have several of the MISSION shRNA clones in the bacterial stocks. I was wondering what the competent cells used are and if you ha...

    shRNA Frequently Asked Questions for Glycerol Stocks

    What should I do if I have trouble obtaining high yields of plasmid DNA? How should I store my bacterial cultures? What is the expected pDNA yield from glycerol stocks? I have several of the MISSION shRNA clones in the bacterial stocks. I was wondering what the competent cells used are and if you ha...

    Lentiviral Production Using X-tremeGENE HP Transfection Reagent

    Lentiviruses represent a powerful tool in research applications to transduce a wide range of cell types. During virus production, low titers and toxicity can be challenging. This easy-to-use protocol uses X-tremeGENE HP Transfection Reagent for fast and robust lentiviral preparation, with high virus...

    Plasmid DNA Purification - Plant Nucleic Acid Purification

    Plasmids are small, circular units of non-genomic DNA that replicate within a cell independently of the chromosomal DNA. Plasmids are most commonly found in bacteria and are used in recombinant DNA research to transfer genes between cells. The Sigma catalog products use overnight, recombinant E. col...

    Lentiviral Systems

    If I want to make my own viral particles, which format should I purchase? What should I do if I have trouble obtaining high yields of plasmid DNA? How many cells can I infect with the amount of lentivirus provided? How does Sigma measure viral titer? What is the method you recommend for screening th...

    Cross Reference of Sigma® GenElute™ to QIAGEN® Nucleic Acid Purification Products

    Faster • 30-minute Maxiprep • 15-minute genomic DNA purification Flexibility • Spin or Vacuum format Cost Effective • More preps in a single kit High Purity • Superior quality for any downstream application - restriction enzyme digest, DNA sequencing, transfection, etc. QIAGEN® Product No. Descripti...