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    Search term:"Sample Preparation"

    506 matches found for Sample Preparation

    LC/MS Analysis of Methamphetamine Enantiomers on Astec® CHIROBIOTIC® V2 in Urine following Liquid/Liquid Extraction

    sample preparation 125 µl urine spiked at 125 ng/mL each enantiomer, add 1 mL diethyl ether, mix/vortex 30 min, centrifuge at 10,000 rpm for 10 min. remove 500 µl aliquot of organic (upper) layer, (evaporate to dryness under nitrogen at 55 C, reconstitute in 500 µl mobile phase, filter through 0.22 ...

    HPLC Analysis of Vinblastine Sulfate on Chromolith® HighResolution RP-18 endcapped 150 x 4.6mm

    sample preparation Transfer USP Vincristine Sulfate RS ( or Assay RS) to a suitable volumetric flask, and dissolve in the Standard solution. column Chromolith(R) HighResolution RP-18 endcapped, 150 x 4.6 mm (Merck p/n 1.52023) (1.52023.0001) column temp. ambient mobile phase [A] Dimethylamine and wa...

    HPLC Analysis of Lapatinib on Chromolith(R) Performance 50-2mm

    sample preparation Methanol & Acetonitrile 50:50 column Chromolith® Performance, 50 x 2 mm (1.52007.0001) column temp. ambient mobile phase [A] 3.48 gm of K2HPO4 in 1 lit water. pH adjusted to 7.5 with OPA; [B] Acetonitrile & Methanol 80:20; gradient 0.0 min A --> 60%, 1.25 min A --> 60%, 4.25 min A...

    Analytical Method: ADMI Color Measurement for Prove

    Materials and reagents: Cat.No. 173016 Spectroquant® VIS Spectrophotometer Prove 100 or Cat.No. 173017 Spectroquant® UV/VIS Spectrophotometer Prove 300 or Cat.No. 173018 Spectroquant® UV/VIS Spectrophotometer Prove 600 Cat.No. 114946 Rectangular cells 10 mm and/or Cat.No. 114944 Rectangular cells 50...

    HPLC Analysis of Aflatoxins in Peanut Paste on Ascentis® Express C18 following SPE using Supel™ Tox AflaZea

    sample preparation Solid Phase Extraction sample/matrix raw peanut paste extraction process suspend 25 g raw peanut paste in 100 mL acetonitrile:water (84:16) extraction solution SPE tube/cartridge Supel Tox AflaZea, 6 mL (55314-U) sample addition directly load 2 mL extract onto SPE tube elution pul...

    UHPLC/MS Analysis of NBOMe Designer Drugs in Urine on Ascentis® Express C18 after Solid Phase Extraction (SPE) using Supel™-Select HLB

    sample preparation SPE (Solid Phase Extraction) sample/matrix human trine spiked at 100 ng/mL each compound SPE well plate Supel-Select HLB 96-well pate, 30 mg/well (575661-U) condition 1 mL methanol followed by 1 mL water sample addition 1 mL washing 1 mL water followed by 1 mL water:methanol (75:2...

    Analytical Method: Aluminium in soils

    Instruments and Reagents: Cat.No. 173016 Spectroquant® VIS Spectrophotometer Prove 100 or Cat.No. 173017 Spectroquant® UV/VIS Spectrophotometer Prove 300 or Cat.No. 173018 Spectroquant® UV/VIS Spectrophotometer Prove 600 or Cat.No. 109748 Spectroquant® Photometer NOVA 30 or Cat.No. 109751 Spectroqua...

    HPLC Analysis of Amlodipine Besylate Tablets on Purospher(R) STAR RP-18 endcapped

    sample preparation Take 10 tablets into a 50-mL volumetric flask, add into 5mL mobile phase, sonicate for 30 minutes. Cool down solution to room temperature, add mobile phase to volume. (Shake well, filter, dilute with mobile phase to a concentration of 50 μg/mL (50 ppm).) column Purospher® STAR RP...

    HPLC Analysis of Nateglinide Tablet on Purospher STAR RP-18 endcapped

    sample preparation Take 10 tablets of Nagelienai Pian, weigh precisely, grind to powder. Weight appropriate amount (roughly contained 70mg Nateglinide ) to a 100-mL volumetric flask, (add appropriate amount of 55% ACN solution. Shake for 30 minutes. Add diluent to volume. Filtrate.) column Purosphe...

    Analytical Method: Chloride in meat and sausage products

    Instruments and Reagents: Cat.No. 173016 Spectroquant® VIS Spectrophotometer Prove 100 or Cat.No. 173017 Spectroquant® UV/VIS Spectrophotometer Prove 300 or Cat.No. 173018 Spectroquant® UV/VIS Spectrophotometer Prove 600 or Cat.No. 109748 Spectroquant® Photometer NOVA 30 or Cat.No. 109751 Spectroqua...

    Analytical Method: Nitrite in soils

    Instruments and Reagents: Cat.No. 173016 Spectroquant® VIS Spectrophotometer Prove 100 or Cat.No. 173017 Spectroquant® UV/VIS Spectrophotometer Prove 300 or Cat.No. 173018 Spectroquant® UV/VIS Spectrophotometer Prove 600 or Cat.No. 109748 Spectroquant® Photometer NOVA 30 or Cat.No. 109751 Spectroqua...

    Analytical Method: Nitrogen (total) according to Kjeldahl (Others)

    Instruments and Reagents: Cat.No. 173016 Spectroquant® VIS Spectrophotometer Prove 100 or Cat.No. 173017 Spectroquant® UV/VIS Spectrophotometer Prove 300 or Cat.No. 173018 Spectroquant® UV/VIS Spectrophotometer Prove 600 or Cat.No. 109748 Spectroquant® Photometer NOVA 30 or Cat.No. 109751 Spectroqua...

    HPLC Analysis of Horny Goat Weed Extract Adulterated with Erectile Dysfunction Drugs on Ascentis® Express Phenyl-Hexyl

    sample preparation Filter with 0.22 µm PVDF (Z227498) sample preparation Sonicate 20 min sample preparation Solvent Desorption extraction Weigh approximately 100 mg of herbal supplement and add 10 mL of 50:50, water:ethanol column Ascentis Express Phenyl-Hexyl, 10 cm x 3.0 mm I.D., 2.7 µm particles ...

    HPLC Analysis of Ergosterol in Vacuum Cleaner Dust on Chromolith(R) Performance 100-2mm

    sample preparation See "Analysis Note." column Chromolith® Performance, 100 x 2 mm (1.52006.0001) column temp. 23 °C mobile phase [A] methanol; [B] Water; (98:2, A:B) flow rate 190 μL/min pressure 595 psi 41 Bar sample Standard: Ergosterol; Matrix: Dust of a vacuum cleaner injection 1 μL detector 28...

    Analytical Method: Aluminium (total) in building materials

    Instruments and Reagents: Cat.No. 173016 Spectroquant® VIS Spectrophotometer Prove 100 or Cat.No. 173017 Spectroquant® UV/VIS Spectrophotometer Prove 300 or Cat.No. 173018 Spectroquant® UV/VIS Spectrophotometer Prove 600 or Cat.No. 109748 Spectroquant® Photometer NOVA 30 or Cat.No. 109751 Spectroqua...

    HPLC Analysis of Voriconazole With Basic Stress on Chromolith Performance 100-3mm

    sample preparation stirring 0.5 hour in NaOH 0.1N column Chromolith® Performance 100 x 3 mm (1.52001.0001) column temp. ambient mobile phase [A] acetonitrile; [B] water; (50:50, A:B) flow rate 425 μL/min pressure 4032 psi (28 bar) sample Voriconazole 50 μg/mL injection 5 μL detector 256 nm cell: 1.4...

    GC Analysis of Phenols in Water on a 5% Phenyl Column after SPE using Supelclean™ ENVI-Chrom P

    sample preparation SPE (Solid Phase Extraction) sample/matrix water spiked with phenol compounds SPE tube/cartridge Supelclean ENVI-Chrom P, 250 mg/6 mL (57225-U) condition 6 mL methyl t-butyl or ethyl acetate; 6 mL acetonitrile. Equilibrate with 6 mL DI water sample addition 100 mL water sample. dr...

    HPLC Analysis of Organic Acids in Beverages on Chromolith® HighResolution RP-18 endcapped

    sample preparation sonicate 5 mL of orange drink for 5 minutes, add 0.2 mL of phosphoric acid solution (1M), dilute to 10 mL with water column Chromolith HighResolution RP-18, 10 cm x 4.6 mm I.D., 2 µm particles (1.52022) column temp. 30 C mobile phase 10 mM diammonium hydrogen phosphate, pH 2.7 flo...

    Analytical Method: Manganese (total) in ores

    Instruments and Reagents: Cat.No. 173016 Spectroquant® VIS Spectrophotometer Prove 100 or Cat.No. 173017 Spectroquant® UV/VIS Spectrophotometer Prove 300 or Cat.No. 173018 Spectroquant® UV/VIS Spectrophotometer Prove 600 or Cat.No. 109748 Spectroquant® Photometer NOVA 30 or Cat.No. 109751 Spectroqua...

    Analytical Method: Nickel (total) in pickling baths (Others)

    Instruments and Reagents: Cat.No. 173016 Spectroquant® VIS Spectrophotometer Prove 100 or Cat.No. 173017 Spectroquant® UV/VIS Spectrophotometer Prove 300 or Cat.No. 173018 Spectroquant® UV/VIS Spectrophotometer Prove 600 or Cat.No. 109748 Spectroquant® Photometer NOVA 30 or Cat.No. 109751 Spectroqua...

    HPLC Analysis of Aflatoxins in Wheat on Ascentis® Express C18 after SPE using Supel™ Tox AflaZea

    sample preparation SPE (Solid Phase Extraction) sample/matrix 25 g wheat meal spiked with pesticide standards derivatization via KOBRA electrochemical cell, 50 muA SPE tube/cartridge Supel(TM)Tox AflaZea, 6 mL (55314-U) sample addition 2 mL elution collect eluate in 5 mL silanized culture tubes elua...

    HPLC Analysis of Nateglinide Tablets on Chromolith HighResolution RP-18 endcapped

    sample preparation Take 10 tablets of Nagelienai Pian, weigh precisely, grind to powder. Weight appropriate amount (roughly contained 70mg Nateglinide ) to a 100-mL volumetric flask, (add appropriate amount of 55% ACN solution. Shake for 30 minutes. Add diluent to volume. Filtrate.) column Chromoli...

    Analytical Method: Copper (total) in electroplating wastewater

    Instruments, Reagents and Accessories: Cat.No. 173016 Spectroquant® VIS Spectrophotometer Prove 100 or Cat.No. 173017 Spectroquant® UV/VIS Spectrophotometer Prove 300 or Cat.No. 173018 Spectroquant® UV/VIS Spectrophotometer Prove 600 or Cat.No. 109748 Spectroquant® Photometer NOVA 30 or Cat.No. 1097...

    LC/MS Analysis of Antidepressants From Urine Using an Ascentis® Express C18 Column

    column Ascentis Express C18, 10cm x 2.1mm, 2.7 µm particles (53823-U) column Ascentis Express C18, 10cm x 2.1mm, 2.0 µm particles (50813-U) column temp. 35 C mobile phase [A] 5 mM ammonium acetate in 0.1% formic acid; [B] 0.1% formic acid in acetonitrile gradient 10% B held for 1 min; to 55% B in 10...

    Analytical Method: Chloride in pickled cabbage

    Instruments and Reagents: Cat.No. 173016 Spectroquant® VIS Spectrophotometer Prove 100 or Cat.No. 173017 Spectroquant® UV/VIS Spectrophotometer Prove 300 or Cat.No. 173018 Spectroquant® UV/VIS Spectrophotometer Prove 600 or Cat.No. 109748 Spectroquant® Photometer NOVA 30 or Cat.No. 109751 Spectroqua...

    LC/MS Analysis of Amphetamine Enantiomers on Astec CHIROBIOTIC® V2 in Urine after Solid Phase Extraction (SPE) using Supel™-Select SCX

    sample preparation SPE (Solid Phase Extraction) sample/matrix 1 mL of synthetic urine spiked with 50 ng/mL of each enantiomer SPE well plate Supel-Select SCX, 96-well plate, 30 mg/well (575664-U) condition 1 mL 1% formic acid in acetonitrile followed by 1 mL water sample addition 1 mL spiked urine s...

    HPLC Analysis of Aflatoxins B1, B2, G1 and G2 in Corn on Ascentis® Express C18 after SPE using Supel™ Tox AflaZea and Fluorescence Detection

    sample preparation SPE (Solid Phase Extraction) sample/matrix 25 g corn meal in 100 mL acetonitrile:water (84:16) SPE tube/cartridge Supel(TM) Tox AflaZea, 6 mL (55314-U) sample addition 2 mL elution collect sample in 5 mL silanized glass vial eluate post-treatment transfer 200 µl sample, add 300 µl...

    LC/MS/MS of 25-Dihydroxyvitamin D2, 25-Hydroxyvitamin D3, and 3-epi-25-Hydroxyvitamin D3 in Serum or Plasma on Ascentis® Express F5 after SPE with HybridSPE®-Phospholipid, Minimization of Matrix Effects

    sample preparation SPE (Solid Phase Extraction) sample/matrix plasma or serum spiked at 25 ng/mL with each vitamin D metabolite SPE well plate 575656-U (HybridSPE-Phospholipid 96-well plate, 50 mg/2 mL) sample addition 100 µl spiked rat plasma followed by 300 µl 1% formic acid in acetonitrile elutio...

    UHPLC/MS Analysis of Spice Cannabinoid Metabolites in Urine on Titan™ C18

    sample preparation SPE (Solid Phase Extraction) SPE well plate SupelSelect HLB 96-Well Plate, 60mg/well (575662-U) condition 1 mL methanol, then 1 mL water sample addition 100 ng/mL in matrix washing 1 mL 20% methanol in water drying none elution 2 mL methanol eluate post-treatment none column Titan...

    Analytical Method: Nitrite pickle salt

    Instrument and Reagents: Cat.No. 173016 Spectroquant® VIS Spectrophotometer Prove 100 or Cat.No. 173017 Spectroquant® UV/VIS Spectrophotometer Prove 300 or Cat.No. 173018 Spectroquant® UV/VIS Spectrophotometer Prove 600 or Cat.No. 109748 Spectroquant® Photometer NOVA 30 or Cat.No. 109751 Spectroquan...