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    Search term:"degradations"

    411 matches found for degradations

    Certified Reference Materials: Synthesis and Characterization of Cocaine N-oxide HCl

    Cocaine is one of the most widespread illicit drugs of abuse in the US. Cocaine N-oxide is a metabolite of cocaine formed by cytochrome P-450 mediated oxidation of cocaine.1,2 Cocaine N-oxide has been reported to be found in human plasma of cocaine users as well as meconium.1 Cocaine N-oxide isomers...

    Impact of collection, stabilization and isolation of bovine ear punches

    1Biomatrica, Inc., 5627 Oberlin Drive, Suite 120, San Diego, CA 92121 USA. 2PrionicsAG, Wagistr. 27a, CH-8952 Schlieren-Zurich, Switzerland. 3Illumina, Inc., 9885 Towne Centre Drive, San Diego, CA 92121 USA. *Corresponding author: swilkinson@biomatrica.com Genetic-based bovine parentage identificati...

    Organic Field Effect Transistors Based on DNTT and Related Organic Semiconductors

    Professor Kazuo Takimiya Emergent Molecular Function Research Group, RIKEN Center for Emergent Matter Science (CEMS), Wako, Saitama 351-0198, Japan FAX: +81-48-462-1473 Department of Applied Chemistry, Graduate School of Engineering, Hiroshima University, Higashi-Hiroshima 739-8527, Japan Introducti...

    Glycan Labeling

    Glycobiology Analysis Manual Table of Contents GlycoProfile™ Products for Metabolic Glycan Labeling FLAG® Antibodies for Detection of FLAG-labeled Glycans GlycoProfile™ Labeling Kits Fluorophores Chromophores Additional Reagents for Reductive Amination Structural modifications of proteins are essent...

    FluoroProfile Kit

    Shed new light on your protein quantification. FluoroProfile™ (Product No. FP0010) is significantly more sensitive than existing colorimetric methods, and exhibits a larger linear dynamic range than other fluorometric methods. Results are achieved in 20 minutes and reversible binding of the fluoroph...

    HPLC Separation of Fluorinated Pharmaceutical Compounds on Supelco Ascentis Columns

    Jacinth A. M. McKenzie and Wayne K. Way wway@sial.com Fluorinated compounds possess distinct characteristics that confer desirable physico-chemical properties compared to their non-fl uorinated analogs. Their abundance in pharmaceuticals, chemicals, consumer products and as environmental pollutants ...

    HumanKine® Thermostable bFGF

    Introduction Methods – Determining stability of bFGF cytokines at elevated temperatures – Culture of human iPS cells – Expansion of ENStem™-A human neural progenitors Results Discussion Materials Fibroblast growth factors (FGFs) are secreted glycoproteins that regulate several fundamental developmen...

    Met

    The Met family of receptor tyrosine kinases (RTKs) contains two members in mammals, referred to as Met and Ron. Met is the receptor for hepatocyte growth factor/scatter factor (HGF/SF), while Ron is the receptor for macrophage stimulating protein (MSP). In the adult, Met is predominantly expressed i...

    Phosphatase Inhibitor Cocktail

    • • Download PDF • Phosphorylation is a reversible protein post-translational modification. Protein kinases catalyze the transfer of the negatively charged γ-phosphate group from ATP to a hydroxyl side chain of serine, threonine or tyrosine residues of the protein, changing the protein’s conformatio...

    insulin-degrading enzyme
    Synonyms INSULYSIN
    Species Human (3416) , Species Mouse (15925) , Species Rat (25700) , Species Zebrafish (561390) , Species domestic cat (101093646) , Species cow (523752) , Species Horse (100071303) , Species dog (477768) , Species chicken (423814) , Species fruit fly (40248) , Species domestic guinea pig (100719651) , Species naked mole-rat (101706921) , Species sheep (101104194)
    Summary: This gene encodes a zinc metallopeptidase that degrades intracellular insulin, and thereby terminates insulins activity, as well as participating in intercellular peptide signalling by degrading diverse peptides such as glucagon, amylin, bradykinin, and kallidin. The preferential affinity of this enzyme for insulin results in insulin-mediated inhibition of the degradation of other peptides such as beta-amyloid. Deficiencies in this protein's function are associated with Alzheimer's disease and type 2 diabetes mellitus but mutations in this gene have not been shown to be causitive for these diseases. This protein localizes primarily to the cytoplasm but in some cell types localizes to the extracellular space, cell membrane, peroxisome, and mitochondrion. Alternative splicing results in multiple transcript variants encoding distinct isoforms. Additional transcript variants have been described but have not been experimentally verified.[provided by RefSeq, Sep 2009]

    Greener Alternatives Evaluation Matrix

    Our approach to evaluating greener chemicals is based on the 12 Principles of Green Chemistry. We recognize that these principles distill into three major categories: improving resource use, more efficient use of energy, and minimizing human and environmental hazards. At present, we are not incorpor...

    Glycan Sequencing Using Exoglycosidases

    • • Download PDF • Glycobiology Analysis Manual Table of Contents Glycan Sequencing Using Exoglycosidases Enzymatic analysis of oligosaccharides using highly specific exoglycosidases, either sequentially or in a matrix array, is a powerful technique in determining the sequence and structure of glyca...

    Yeast Transformation Protocols

    Yeasts are considered model systems for eukaryotic studies as they exhibit fast growth and have dispersed cells. Moreover, replica plating and mutant isolation of yeast cells can be done with relative ease and they have a well-defined genetic system. Most significantly, yeasts have a highly versatil...

    Hops (Humulus lupulus) | Plant Profiler

    Synonyms / Common Names / Related Terms Colupulone, common hops, European hops, hop, hop strobile, Hopfen (German), houblon (French), humulon, humulus, Humulus lupulus, iso-alpha-acids, lupulin, lupulus, Lupuli strobulus (German), prenylated 2`-hydroxychalcones, prenylflavonoids, spent hops, xanthoh...

    Ginseng (Panax ginseng) | Plant Profiler

    Synonyms / Common Names / Related Terms Ginseng (American ginseng, Asian ginseng, Chinese ginseng, Korean red ginseng, Panax ginseng:Panaxspp., includingP. ginsengC.C. Meyer andP. quinquefoliusL., excludingEleutherococcus senticosus), Acetylenic alcohol, acidic polysaccharides, acupuncture-moxibusti...

    Baseclick EdU Cell Proliferation Detection Kits: Improve Data Quality and Save Time

    Experimental studies measuring cell proliferation have had implications in cancer biology, immunology, cell biology, and developmental biology. Various methods for monitoring cell proliferation have been developed. Counting cells using a hemocytometer is one of the simplest methods used to monitor c...

    Improving Reproducibility: Best Practices for Antibodies

    In the midst of beeping lab timers, presentations and grant deadlines, it is easy to take for granted the quality of lab reagents. Recent headlines have highlighted the risks of not validating reagents prior to experimentation, with many stories about wasted years of work, false hopes of reproducing...

    Micro and Nanometer Scale Photopatterning of Self-Assembled Monolayers

    Graham J. Leggett* Department of Chemistry, University of Sheffield, Brook Hill, Sheffield S3 7HF, United Kingdom *Email: Graham.Leggett@shef.ac.uk Self-assembled monolayers (SAMs) have attracted enormous interest for a wide variety of applications in micro- and nano-technology. In this article, we ...

    Phosphatase Inhibitor Cocktail | Biowire Fall 2010 - A Look into the Future of Gene Editing

    Subscribe to Biowire and eBiowire Biowire Fall 2010 — A Look into the Future of Gene Editing Download Biowire Fall 2010 (16 Mb PDF) Table of Contents • Genome by Design – Dave Smoller • ZFN Technology • An Interview with Dana Carroll • Tech Tip: ZFN Donor Design • Study Spotlights ○ Development of T...

    Biodegradable Polyester Based Nanoparticle Formation by Miniemulsion Technique

    Aliphatic polyesters such as polylactide, poly(lactide-co-glycolide) and polycaprolactone, as well as their copolymers, represent a diverse family of synthetic biodegradable polymers that have been widely explored for medical uses and are commercially available.1 These polyesters have excellent tiss...

    Research Essentials - Technical Library

    Buffer pKa/pH Tables and Formulas: Useful pH Ranges of Selected Biological Buffers (25 °C, 0.1M) Trizma Buffer Table - pH vs. Temperature Phosphate Buffer Table - 0.2M solution Commonly Used Electrophoresis Buffers Helpful Formulas DNA / Protein Electrophoresis and Troubleshooting Tables: Effective ...

    Microreactor Technology

    • • Download PDF • Matthias Junkers Product Manager Within the past two decades, Microreactor Technology (MRT) has evolved from a highly advanced toy for chemical engineers to a versatile tool for chemical synthesis. Since the time of the founders of synthetic chemistry, like Justus von Liebig or Fr...

    Superior Fluorescent Imaging Using Atto Dyes

    • • Download PDF • Activated fluorescent dyes are routinely used to tag proteins, nucleic acids, and other biomolecules for use in life science applications including fluorescence microscopy, flow cytometry, fluorescence in situ hybridization (FISH), receptor binding assays, and enzyme assays. The A...

    Injectable Hydrogels for Cell Delivery and Tissue Regeneration

    Prathamesh M. Kharkar,1 April M. Kloxin1,2* 1Materials Science and Engineering and 2Chemical and Biomolecular Engineering University of Delaware, Newark, DE 19716 USA *Email: akloxin@udel.edu The use of hydrogel-based biomaterials for the delivery and recruitment of cells to promote tissue regenerat...

    Sialic Acid Synthesis and Signaling | BioFiles Volume 5, Number 1 Glycobiology

    BioFiles Volume 5, Number 1 — Glycobiology Download Biofiles v5 n1 (4.4 Mb PDF) Table of Contents • Introduction • Sialic Acid Synthesis and Signaling • Mass Spectrometry of Glycans • Histological Detection of Pathogenic Fungi in Human Tissue • Select-HA™ • New Products for Glycobiology and Glycoche...

    Alternative Energy Tutorial

    Introduction Batteries Fuel Cells Conducting Polymers High-Purity Inorganics Liquid Electrolytes Plasticizers and Binders Solid Polymeric Electrolytes References Fuel cells and batteries are electrochemical cells used to generate an external electrical current. They consist of an anode, where oxidat...

    Balancing Histone/Protein Acetylation: Gene Regulation and Cancer Therapy

    • • Download PDF • Michigan State University, East Lansing, Michigan, USA A highly sophisticated mechanism organizes the 2-meter long human genome within a 6-μm wide nucleus. Up to 10,000-fold of compaction of the chromatin is achieved during mitosis, allowing faithful segregation of mitotic chromos...

    Protocols for Membrane Scaffold Proteins and Nanodisc Formation

    The following material related to Nanodisc Technology is adapted from on-line content of the research group of Professor Stephen Sligar of the University of Illinois at Urbana-Champaign, with the kind permission of Professor Sligar. The concept of membrane scaffold proteins was originally derived fr...

    Glycosaminoglycans and Proteoglycans

    Glycobiology Analysis Manual Table of Contents Glycosaminoglycans Proteoglycans and Associated Proteins Glycosaminoglycans (GAGs) are large linear polysaccharides constructed of repeating disaccharide units with the primary configurations containing an amino sugar (either GlcNAc or GalNAc) and an ur...

    Andrographis (Andrographis paniculata) | Plant Profiler

    Synonyms / Common Names / Related Terms Andrographolide, Chuan Xin Lin, Green chiretta, Kalmegh leaf extract, Kan Jang®, King of Bitters, Kold Kare®, Remdex®. Mechanism of Action Pharmacology: • Constituents: Andrographolide, a diterpene lactone compound, is believed to be the principal active agent...

    Neuropsychiatric Disorders and Cell Signaling

    • Schizophrenia, the most common psychotic disorder, affects ~ 25 million people worldwide and is one of the leading causes of years lived with disability.1 Historically, antipsychotic drug discovery has been serendipitous, resulting in treatment of a subset of symptoms while failing to address othe...