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    Search term:"gene_expression"

    939 matches found for gene_expression

    Nitric Oxide Synthases

    Nitric oxide is a reactive free radical gas that can act as an intracellular or extracellular messenger. It may act locally as an autacoid, paracrine substance or neurotransmitter, and at a distant target if it is carried and delivered as a protected complex, or prodrug. It is therefore, a very uniq...

    Nuclear Receptors (Non-Steroids)

    The receptors for several classes of non-steroidal compounds, including retinoids, thyroid hormones and vitamin D, also act as ligand-dependent transcription factors. In addition, a large number of receptors without known ligands have been discovered based on sequence homology, particularly in the D...

    Nuclear Receptors (PPARs)

    Peroxisome proliferator activated receptors (PPARs) are members of the nuclear hormone receptor superfamily of ligand-activated transcription factors that are related to retinoid, steroid and thyroid hormone receptors. PPARs play an important role in many cellular functions including lipid metabolis...

    Commercially Available Serum-Free Insect Media: A Comparison of Sf9 Growth Dynamics and Protein Production

    Diane E. Potts1, Justine A. Malinski1, Laura T. Kakach1 and Sara L. Gilliland2 1 ATG Laboratories, Inc., Suite 107, 10300 Valley View Road, Eden Prairie, MN 55344 2 SAFC, 13804 West 107th Street, Lenexa, KS 66215 Abstract Introduction Materials Serum-Free Media Serum-Containing Media Protein Express...

    Nuclear Receptors (Steroids)

    Intracellular receptors (IRs) are a class of ligand-dependent transcription factors that include receptors for both steroid and non-steroid hormones. Upon binding their cognate hormone, these receptors homo- or hetero-dimerize and regulate gene expression through multiple mechanisms. Ligand-bound IR...

    Streamlining the Biological Factory: Making a Better CHO Cell for Biopharma Production

    Developing the CHOZN Platform What the Technology Implies View the PDF SAFC’s CHOZN® Platform has been developed as a ‘plug and play’ manufacturing system for the creation of biopharmaceutical producing cell lines. The design criteria were initially focused on the production of therapeutic monoclona...

    Sodium Channels

    Voltage-gated sodium channels are present in most excitable cell membranes and play an important role in generating action potentials. A variety of toxins and chemicals are known to either block or modulate sodium channels and have proven invaluable in investigating the physiological characteristics...

    PAF Receptors

    Following the identification of Platelet-Activating Factor (PAF) in 1972, and structural confirmation in 1979, there was intense interest in the biology of this phospholipid. These early studies demonstrated that PAF is released directly from cell membranes and mediates a wide range of potent and sp...

    Simplicon™ RNA Reprogramming

    Introduction Methods Results Discussion Materials References A ready source of induced pluripotent stem cells (iPSCs) is critical to the effective study of differentiation pathways and the investigation of the therapeutic potential of iPS cells. Since the discovery that human iPSCs could be generate...

    meiosis/spermiogenesis associated 1
    Synonyms SPATA39, bA2K17.3
    Species Human (644890) , Species Mouse (104362) , Species Rat (679834) , Species Zebrafish (541343) , Species domestic cat (101089778) , Species cow (617353) , Species dog (100684014) , Species chicken (769589) , Species domestic guinea pig (100728794) , Species naked mole-rat (101699891) , Species sheep (101109199)

    Transient Receptor Potential Channels

    The transient receptor potential family of ion channels consists of at least 28 mammalian members divided into six subfamilies: 7 TRPC (canonical), 6 TRPV (vanilloid), 8 TRPM (melastatin), 1 TRPA (ankyrin), 3 TRPP (polycystin) and 2 TRPML (mucolipin). Numerous invertebrate TRP channels have also bee...

    BioFiles Volume 4, Number 6 | Nutrition Research

    Nutrition Research Table of Contents Introduction Vitamins Download this Article • Vitamin D and Prevention of Human Disease • Vitamin D, Multiple Sclerosis Connection • Vitamin Related Products • Antibodies for Vitamin Function and Metabolism Plant Bioactive Extraction and Isolation • Acids and Bas...

    CompoZr® Targeted Integration Kits site integration and expression

    Now Available! Targeted Integration into the Rat Genome at the Rosa26 Locus CompoZr Targeted Integration Kits are designed to rapidly integrate a user-specified gene of interest (GOI) into preferred sites of integration in either the human, mouse or rat genome. In the human genome the adeno-associat...

    Whole Transcriptome Amplification Sequencing Technology (SEQR)

    The SeqPlex RNA Amplification Kit for whole genome transcriptome amplification (WTA) is designed to facilitate next-generation sequencing (NGS) of extremely small quantities of degraded/highly fragmented RNA including non-polyA-tailed RNA. The yields from serum, single cells, RNA immunoprecipitation...

    Phosphodiesterases

    Cyclic nucleotide phosphodiesterases (PDEs) catalyze the hydrolysis of cAMP and/or cGMP. They function with adenylyl and guanylyl cyclases to regulate the amplitude and duration of responses triggered by the second messengers, cAMP and cGMP. In doing so they regulate a wide range of biological respo...

    HumanKine® Thermostable bFGF

    Introduction Methods – Determining stability of bFGF cytokines at elevated temperatures – Culture of human iPS cells – Expansion of ENStem™-A human neural progenitors Results Discussion Materials Fibroblast growth factors (FGFs) are secreted glycoproteins that regulate several fundamental developmen...

    Validation & Guarantee - MISSION shRNA FAQs

    Do you guarantee knockdown using your clones? Yes. Based upon the design algorithm developed by The Broad Institute, at least one clone per target set (set of 4-5 clones) should yield greater than 70% knockdown of the targeted transcript. Validation of the library is underway. Generally more than on...

    Strauss Group – Products available at Aldrich from the Strauss Laboratory

    Professor Product Portal Index Professor Steven Strauss The Strauss group has been among the leaders in the synthesis and applications of new superweak anions for the past three decades. Superweak anions (a term coined by us in 1998 in connection with an ACS Symposium on weakly-coordinating anions),...

    Online Enzyme Quiz Answers

    Your answers were submitted successfully. You have completed our Online Enzyme Quiz! Look out for your confirmation email over the next few days. See below for quiz answers. Return to mainpage. 1. There are a large number of proteases offered among Sigma’s protein products. Which protease is extract...

    Multiplex Real-Time PCR

    Multiplex qPCR employing probe-based chemistries is a demanding application that often requires extensive optimization and validation. The combination of the multi-channel Eppendorf® Mastercycler® ep realplex4 S realtime PCR cycler and KAPA PROBE FAST qPCR Kits from Kapa Biosystems provides an indus...

    Ginseng (Panax ginseng) | Plant Profiler

    Synonyms / Common Names / Related Terms Ginseng (American ginseng, Asian ginseng, Chinese ginseng, Korean red ginseng, Panax ginseng:Panaxspp., includingP. ginsengC.C. Meyer andP. quinquefoliusL., excludingEleutherococcus senticosus), Acetylenic alcohol, acidic polysaccharides, acupuncture-moxibusti...

    Improving Reproducibility: Best Practices for Antibodies

    In the midst of beeping lab timers, presentations and grant deadlines, it is easy to take for granted the quality of lab reagents. Recent headlines have highlighted the risks of not validating reagents prior to experimentation, with many stories about wasted years of work, false hopes of reproducing...

    Neural Lineage Markers

    • • Download PDF • The ever evolving field of stem cell research has been a highly fascinating yet contested area of study. Stem cell research holds promise for many areas such as developmental biology, therapeutic and regenerative medicine, and drug discovery. Potton et al. described stem cells as ...

    Primers for Plasmid Sequencing

    We have designed a range of forward and reverse sequencing primers that allow you to sequence any insert that you make into a particular position within any of our SnapFast™ expression vectors. Other vectors will require that you design your own primers. Where possible, the binding sites for each of...

    MISSION® esiRNA - Endoribonuclease-prepared siRNAs

    On this page: MISSION esiRNA Benefits esiRNA Targeting Long Non-coding RNA esiRNA Screening Video Tutorial esiRNA FAQ (Frequently Asked Questions) MISSION esiRNA Offering Ordering Information esiRNA Controls References esiRNA Technical Overview (921 Kb PDF) MISSION® esiRNA MISSION esiRNA provides RN...

    Phosphoinositide Kinases

    The membranes of all metazoan cells contain a substantial amount of phosphatidylinositol (PtdIns) and at least seven phosphorylated PtdIns derivatives (PPIn) at 30- to 1000-fold lower concentrations. Phosphoinositide kinases synthesize the various PPIn by adding phosphate groups to pre-existing inos...

    Glycine Receptors

    The amino acid glycine is a major inhibitory neurotransmitter in the vertebrate CNS. Glycinergic synapses are particularly abundant in spinal cord and brain stem, but are also found in higher brain regions including the hippocampus. The inhibitory actions of glycine are potently blocked by the alkal...

    How to Choose the Right Cell Line for Your Research

    How to Choose the Right Cell Line for Your Research Cell lines remain the workhorses of laboratory research, both in academic and industrial settings. This webinar will focus on best practice in selecting and maintaining cell lines for research, to ensure research outputs are relevant and reproducib...

    Acetylcholine Receptors (Muscarinic)

    Muscarinic acetylcholine receptors are members of the superfamily of G protein-coupled receptors (GPCRs). They are relatively abundant and mediate many of the diverse actions of acetylcholine in the CNS, as well as throughout non-nervous tissues innervated by the parasympathetic nervous system. Five...

    Enzyme Reagents CPS Flag

    Detection of FLAG-tagged (FLAG-BAP) protein in an E. coli K12 extract. Procedure: The procedure and illustration below demonstrate detection from 10 nanograms down to 40 picograms of the FLAG-BAP control protein loaded on a 4-20 % Tris-Glycine SDS-PAGE gel utilizing the CPS-1 substrate. 1. The examp...

    Guide to E. coli Markers in Molecular Biology

    In the descriptions below, the markers are described as the genes or gene products. dam Methylates A of GATC sites. The dam methyl pattern is used to distinguish old (methylated) from new (unmethylated) strands for damage-repair purposes (repair the unmethylated strand). Dam methylation is also used...