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    Search term:"sonication"

    52 matches found for sonication

    Graphene Nanoribbons: Production and Applications

    Ayrat M. Dimiev1 and James M. Tour2 1EMD Performance Materials Corp., 70 Meister Ave, Somerville, NJ 08876 USA 2Departments of Chemistry, Materials Science and NanoEngineering, and Computer Science, and the Smalley Institute for Nanoscale Science and Technology Rice University, MS-222, 6100 Main Str...

    Handling and Storage of Synthetic Peptides

    One of the most challenging aspects of working with synthetic peptides is determining the best solvent in which to dissolve the peptide. General guidelines for proper storage, handling and dissolving your synthetic peptide are provided below. Lyophilized peptides are shipped at ambient temperature a...

    Solid Phase Extraction: Normal Phase Methodology

    Basic Steps Common Normal-Phase Solvents In order for polar retention to occur between the sorbent and the sample, the analyte must be introduced to the SPE device in a non-polar sample or mobile phase environment. Therefore, typical sample matrices that can be employed in normal-phase SPE include h...

    Sera-Mag™ and Sera-Mag SpeedBeads Magnetic Particles

    Introduction Sera-Mag Magnetic SpeedBeads Sera-Mag Magnetic Particles Materials Sera-Mag and Sera-Mag SpeedBeads provide cost effective magnetic bead separation technology for molecular biology applications, nucleic acid isolation, and research immunoassays. Sera-Mag superparamagnetic particles comb...

    Carbon Nanomaterials: Elemental Analyses and Quantification of Their Accumulation in Living Cells

    Introduction Elemental Analyses of CNMs Benefits of an Extensive Elemental Analysis of CNMs Quantifying CNMs Accumulated by Biological Cells Gel Electrophoresis Method to Quantify CNMs Gel Electrophoresis Method to Quantify CNMs in Aqueous Samples Gel Electrophoresis Method to Quantify CNMs Extracte...

    Sample Preparation & Gel Electrophoresis: Western Blot Troubleshooting

    Electric currents, wires, leads, combs, leaks… so many opportunities for trouble. But we still use gels, because electrophoresis remains an effective way to separate proteins — so that the results of antibody-based immunodetection can be fairly unequivocal. Click on the Sample Preparation & Gel Elec...

    Separation of Ginsenosides in Panax Quinquefolius (American Ginseng)

    Related Information • Analytix 3, 2015 (PDF) Belonging to the plant genus panax, ginseng is one of the most well-known herbal medicines. It has been used in traditional Chinese medicine for centuries, but has also become very popular in the Western world. Medicines derived from the ginseng root are ...

    Melamine and Related Compounds in Dog Food Using GC-MS

    Related Articles • Fast Extraction and Low Level Detection of Chloramphenicol in Shrimp • Extraction and Analysis of PAHs from Grilled Hamburger Melamine contamination in food became an issue in recent years after the discovery of it, and related compounds, in pet food and baby formula. It was disco...

    Manufacturing, Characterization and Use of Single Walled Carbon Nanotubes

    • • Download PDF • SouthWest NanoTechnologies, Inc. 2501 Technology Place Norman, OK 73071 *Email: pwallis@swentnano.com Carbon nanotubes are materials that possess remarkable properties and offer extraordinary possibilities. This article gives a brief overview of the physico-chemical nature and cha...

    sonic hedgehog
    Synonyms HHG1, HLP3, HPE3, MCOPCB5, SMMCI, TPT, TPTPS
    Species Human (6469) , Species Mouse (20423) , Species Rat (29499) , Species chicken (395615) , Species domestic cat (100169727) , Species dog (608860) , Species cow (286821) , Species sheep (780492) , Species sheep (101117666) , Species domestic guinea pig (100732014) , Species naked mole-rat (101719896) , Species Domestic Rabbit (100352774)
    Summary: This gene encodes a protein that is instrumental in patterning the early embryo. It has been implicated as the key inductive signal in patterning of the ventral neural tube, the anterior-posterior limb axis, and the ventral somites. Of three human proteins showing sequence and functional similarity to the sonic hedgehog protein of Drosophila, this protein is the most similar. The protein is made as a precursor that is autocatalytically cleaved; the N-terminal portion is soluble and contains the signalling activity while the C-terminal portion is involved in precursor processing. More importantly, the C-terminal product covalently attaches a cholesterol moiety to the N-terminal product, restricting the N-terminal product to the cell surface and preventing it from freely diffusing throughout the developing embryo. Defects in this protein or in its signalling pathway are a cause of holoprosencephaly (HPE), a disorder in which the developing forebrain fails to correctly separate into right and left hemispheres. HPE is manifested by facial deformities. It is also thought that mutations in this gene or in its signalling pathway may be responsible for VACTERL syndrome, which is characterized by vertebral defects, anal atresia, tracheoesophageal fistula with esophageal atresia, radial and renal dysplasia, cardiac anomalies, and limb abnormalities. Additionally, mutations in a long range enhancer located approximately 1 megabase upstream of this gene disrupt limb patterning and can result in preaxial polydactyly. [provided by RefSeq, Jul 2008]

    Protease Inhibitor Cocktails References

    The Sigma-Aldrich portfolio of protease inhibitor cocktails shows an excellent track record in the peer-reviewed scientific literature, as witnessed by the many publications that cite our protease inhibitor cocktails. This page lists a sampling of those research papers. Other citations are available...

    Peptide Solubility

    Improper solubilization can potentially result in loss of the peptide/protein and result in failure of the experiment. When available, the solubility information may be found on the product information page or on the Certificate of Analysis. If the solubility information is not known, there are at l...

    Carbon Nanofibers- Processing and Dispersing

    Carbon nanofibers (CNFs) are discontinuous, highly graphitic, highly compatible with most polymer processing techniques, and can be dispersed in an isotropic or anisotropic mode. They have excellent mechanical properties, high electrical conductivity, and high thermal conductivity, which can be impa...

    Sampling Ammonia using radiello Passive Sampler

    The following article was generated with the assistance of an outside source using Sigma-Aldrich® products. Content was provided from the public information published on the Ammonia Monitoring Network website. http://nadp.isws.illinois.edu Introduction The National Atmospheric Deposition Program (NA...

    Polytriarylamine Semiconductors

    • • Download PDF • Flexink Limited - www.flexink.co.uk Southampton, UK E-mail: sales@flexink.co.uk Organic electronics is an emerging scientific field of potentially huge technological and commercial relevance1, and is an increasingly ubiquitous research activity globally. One important area is the ...

    A Step-by-Step Guide for Solution Based Self-Assembly

    Material Matters 2006, 1.2, 18. Dr. Dan Graham, Assemblon; Dr. Sean Dingman, Sigma-Aldrich Self-assembled monolayers of thiols are prepared by immersion of a clean gold substrate into a dilute solution of the desired thiol. Although self-assembly takes place very quickly, good experimental procedure...

    ChIP Troubleshooting Questions

    • On page 3 of the bulletin, the tissue prep procedure calls for making the cross-link solution with formaldehyde in cell culture medium but there is no mention of cell culture medium anywhere else and why would this be used instead of an extraction buffer? What else can be used? • What if there are...

    Molecular Monolayers on Silicon Surfaces

    • • Download PDF • Steacie Institute for Molecular Sciences, 100 Sussex Dr., Ottawa, Ontario, Canada E-mail: Gregory.Lopinski@nrc-cnrc.gc.ca Gregory P. Lopinski* and Daniel D.M. Wayner Controlled formation of organic molecular monolayers on silicon surfaces offers the promise of enhancing the functi...

    Graphene-Based Transparent Conductive Electrodes

    Kehan Yu1 and Junhong Chen2* 1Department of Chemical Engineering Case Western Reserve University Cleveland, Ohio 44106 2Department of Mechanical Engineering University of Wisconsin-Milwaukee, Milwaukee, Wisconsin 53211 *jhchen@uwm.edu A transparent conductive electrode (TCE) is an essential componen...

    Single Walled Carbon Nanotubes

    Richard Jansen and Philip Wallis* SouthWest NanoTechnologies, Inc. 2501 Technology Place Norman, OK 73071 *Email: pwallis@swentnano.com Carbon nanotubes are materials that possess remarkable properties and offer extraordinary possibilities. This article gives a brief overview of the physico-chemical...

    Array CGH Analysis of Challenging Samples

    In recent years, array-based Comparative Genomic Hybridization (aCGH) has been refined to determine chromosomal changes at progressively higher resolutions1. This evolving technology is, however, hampered by the large DNA input requirement—a minimum of 150,000 copies of a human genome, or 0.5 μg, ar...

    Preparing Self-Assembled Monolayers

    Self-assembled monolayers (SAMs) of thiols are prepared by immersing a clean gold substrate into a dilute solution of the desired thiol. Figure 1. Schematic showing an ordered self-assembled monolayer of densely packed alkane thiols. Although self-assembly takes place rapidly, good experimental proc...

    Frequently Asked Questions - radiello® Products

    On this page: • General • Diffusive Bodies • RAD170 for Hydrogen Sulfide • RAD145 for BTEX/VOCs • RAD130 for BTEX/VOCs See Also: • What is passive diffusive sampling? • What is radiello®? • How to use radiello® • Applications What is the purpose of the recommended extra storage tubes? The extra stor...

    Peptide Quick Tips

    Common impurities found in custom peptide material can include any of the following: • Sequences with amino acid deletions • Incomplete peptide sequences (truncation) • Sequences containing incompletely deprotected amino acids • Sequences modified as a result of the cleavage process (i.e. reattachme...

    Perparing Cell Lysates: Western Blot Troubleshooting

    Related Links • Western Blotting (Immunoblotting) Protocol • 30-minute Immunodetection Protocol Using the SNAP i.d.® 2.0 System • Sample Preparation Methods from GE Healthcare • Products for Cell Lysis and Protein Extraction • Western Blotting Home Page • Western Blotting Literature & Resources • We...

    Preparation of Bottom-Gate OFET using P3HT

    Plexcore OS 1100, a p-type polymer semiconductor, is a moderate molecular weight grade of P3HT that is optimized for Organic Field Effect Transistor (OFET) applications. It has been used successfully in top and bottom-gate OFET architectures with a variety of device dimensions, substrates, and diele...

    Enzymatic Assay of Urease from Jack Beans

    To standardize a procedure for the enzymatic assay of Urease, from Jack Beans. The scope of this procedure is for all products that have a specification for the enzymatic determination of Urease, from Jack Beans. This assay is not to be used to determine the activity of Urease, from Bacillus pasteur...

    Thermogelation of PLGA-block-PEG-block-PLGA Copolymers

    Related Articles • RESOMER® Biodegradable Polymers • Degradable Poly(ethylene glycol) Hydrogels for 2D and 3D Cell Culture • Preparation of Chitosan Microparticles by Spray Drying • Biodegradable Polyester Based Nanoparticle Formation by Miniemulsion Technique • Dendrons and Hyperbranched Polymers: ...

    Western Blotting – Sample Preparation

    • Introduction • Sample preparation Symbols This symbol indicates general advice on how to improve procedures or recommends measures to take in specific situations ** indicates a third party trademark The importance of good sample preparation cannot be stressed too highly. By understanding the natur...

    Single-Walled Carbon Nanotubes: Exfoliation and Debundling Procedure

    The dispersibility and bundle defoliation of single-walled carbon nanotubes (SWCNTs), which can be applied to materials produced by the CoMoCAT® process, have been extensively investigated by SouthWest NanoTechnologies (SWeNT ®) and at the University of Oklahoma.1 The procedure employed to disperse ...

    Selected Applications of Metal-Organic Frameworks in Sustainable Energy Technologies

    • • Download PDF • Stephen R. Caskey Adam J. Matzger Stephen R. Caskey1 and Adam J. Matzger2 1Sigma-Aldrich Corp., Milwaukee, WI 53209 2Department of Chemistry and the Macromolecular Science and Engineering Program, University of Michigan, Ann Arbor, MI 48109 Email: stephen.caskey@sial.com, matzger@...