Dyes and Labels

Biotin Phosphoramidite

Biotin-labeled oligonucleotides have become extremely popular in DNA capture and detection applications, due to their very high sensitivity and strong, specific binding affinity with avidin and streptavidin proteins. Biotin-based detection methods are widely used by researchers. Biotin labeling is compatible with PCR and most hybridization techniques.

Key Features of the Biotin Phosphoramidite
  • Behaves like any standard amidite on the DNA synthesizer
  • Readily soluble in acetonitrile
  • Biotin compound has a dimethozytrityl (DMT) — group on the ring structure, which allows coupling-yield determination and trityl-selective purification
  • A long, water-compatible linker arm ensures maximum sensitivity
  • The labeled oligonucleotide is ready for use in most applications after the evaporation of ammonia. Standard procedures can be used if additional purification is required
  • Provides a coupling efficiency of 95%

Product #

Structure

Description

Formula

Compatibility

Pkg Qty

Add to Cart

M01038-HH Biotin Phosphoramidite C46H64N5O8PS configured for PerkinElmer 8900
configured for PerkinElmer
configured for Polygen
pkg of 1 × 0.25 g (M010382-01)
M042000 Biotin Phosphoramidite C46H64N5O8PS    
M042030 Biotin Phosphoramidite C46H64N5O8PS configured for ABI
 
M042080 Biotin Phosphoramidite C46H64N5O8PS configured for PerkinElmer
configured for Polygen
 
M042100 DMT-Biotin-C6 Phosphoramidite C46H64N5O6PS    
M042180 DMT-Biotin-C6 Phosphoramidite C46H64N5O6PS configured for PerkinElmer
configured for Polygen
 
M042130 DMT-Biotin-C6 Phosphoramidite C46H64N5O6PS configured for ABI
 

Cyanine 3 Phosphoramidite

Product #

Description

Add to Cart

M047000 Cyanine 3 Phosphoramidite
M047030 Cyanine 3 Phosphoramidite configured for ABI
M047080 Cyanine 3 Phosphoramidite configured for PerkinElmer, configured for Polygen

Cyanine 5 Phosphoramidite

Product #

Structure

Description

Formula

Compatibility

Pkg Qty

Add to Cart

M046030 Cyanine 5 Phosphoramidite C60H72N4O4P configured for ABI ÄKTA® and OligoPilot®
 
M046080 Cyanine 5 Phosphoramidite C60H72N4O4P configured for PerkinElmer
configured for Polygen
 

Fluorescein Phosphoramidite

Fluorescein phosphoramidite is composed of a protected fluorescein molecule linked to a ß-cyanoethylphosphoramidite. The fluorescein derivative consists of to isomers derived form 5- and 6- carboxy fluorescein. As a result, two peaks will be seen on a RP-HPLC chromatogram of the synthesis product.

Key Features of the Fluorescein Phosphoramidite
  • Readily soluble in acetonitrile
  • Couples to the 5’ end of the oligonucleotide using standard synthesis protocols
  • Protecting groups on the fluorescein moiety are removed under standard conditions of cleavage and deprotection with concentrated ammonia
  • A detritylation step is not required since the fluorescein phosphoramidite does not contain a dimethoxytrityl (DMT-) group
  • The labeled oligonucleotide is ready for use in most applications after the evaporation of ammonia. Standard procedures can be used if additional purification is required
  • The fluorescein moiety provides a purification handle in RP-HPLC purification

Product #

Structure

Description

Formula

Compatibility

Pkg Qty

Add to Cart

M041080 Fluorescein Phosphoramidite C46H58N3O10P configured for PerkinElmer
configured for Polygen
 
M041030 Fluorescein Phosphoramidite C46H58N3O10P configured for ABI
 

6-Fluorescein Phosphoramidite

Key Features of 6-FAM
  • 6-FAM consists of one isomer derived from 6-carboxy fluorescein
  • Couples to the 5′-end of the oligonucleotide using standard synthesis protocols
  • Protecting groups on the fluorescein moiety are removed under standard conditions of cleavage and deprotection with concentrated ammonia
  • A detritylation step is not required since the fluorescein phosphoramidite does not contain a DMT-group
  • The labeled oligonucleotide is ready for use in most applications after the evaporation of ammonia—standard procedures can be used if additional purification is required
  • Lot-to-lot consistent purity and performance
  • Manufactured under a certified ISO 9001 quality system since 1993

Product #

Structure

Description

Formula

Compatibility

Pkg Qty

Add to Cart

M041100 6-Fluorescein Phosphoramidite C46H58N3O10P    
M041130 6-Fluorescein Phosphoramidite C46H58N3O10P configured for ABI
 
M041180 6-Fluorescein Phosphoramidite C46H58N3O10P configured for PerkinElmer
configured for Polygen
 

6-Hexachloro-Fluorescein Phosphoramidite

Product #

Structure

Description

Formula

Compatibility

Pkg Qty

Add to Cart

M043130 6-Hexachloro-Fluorescein Phosphoramidite C46H52Cl6N3O10P configured for ABI
 
M043180 6-Hexachloro-Fluorescein Phosphoramidite C46H52Cl6N3O10P configured for PerkinElmer
configured for Polygen
 

Phosphate-On Phosphoramidite

Phosphate-ON phosphoramidite is a chemical phosphorylation reagent that can be employed to introduce a phosphate group at the 5’ terminus of an oligonucleotide. Chemical phosphorylation is a cost effective alternative to enzymatic phosphorylation methods, allowing introduction of terminal phosphate groups in high yields, at any desired scale.
Proligo Reagents’ offers Phosphate-ON phosphoramidite in powder form, enabling the dissolution of the reagent in acetonitrile to be easily monitored. The application of the reagent is fully compatible with standard cleavage and deprotection procedures at the end of oligonucleotide synthesis. Phosphate-ON phosphoramidite from Proligo Reagents comprises a thymidine nucleoside, which is removed from the oligonucleotide during the cleavage and deprotection process.

Key Features of 5’-Phosphorylated Oligonucleotides
  • Enables enzymatic ligation to the 3′- terminus of nucleic acids
  • Offers protection against exonucleolytic degradation
  • Provides oligonucleotide conjugation through activation with coupling reagents such as EDC

These features enable 5’-phosphorylated oligonucleotides to be used in applications such as molecular cloning and gene construction; ligase chain reaction; template directed ligation; oligonucleotide labeling with reporter groups, haptens and other modifiers; and oligonucleotide stabilization.

Key Features of Phosphate-ON Phosphoramidite
  • Fully compatible with standard phosphoramidite reagents and synthesis conditions
  • Compatible with dG(dmf) fast deprotection chemistry: the oligonucleotide can be cleaved from the support and deprotected in concentrated ammonia at 55°C in only 2 hours
  • Comprises a DMT-group for trityl monitoring
  • Dissolves easily in acetonitrile to a 0.1M solution
  • Powder format enables dissolution to be easily monitored and facilitates product handling
  • High coupling efficiency of >= 98.0% under standard DNA phosphoramidite coupling conditions
  • Cleavage of both phosphate protective groups through a ß-elimination process

Product #

Structure

Description

Formula

Compatibility

Pkg Qty

Add to Cart

M061000 Phosphate-ON Phosphoramidite C49H62N7O11P    
M061030 Phosphate-ON Phosphoramidite C49H62N7O11P configured for ABI
 
M061040 Phosphate-ON Phosphoramidite C49H62N7O11P configured for MerMade
 
M061080 Phosphate-ON Phosphoramidite C49H62N7O11P configured for PerkinElmer
configured for Polygen
 

6-Tetrachloro-Fluorescein Phosphoramidite

Product #

Structure

Description

Formula

Compatibility

Pkg Qty

Add to Cart

M045030 6-Tetrachloro-Fluorescein Phosphoramidite C46H54N3O10Cl4P configured for ABI
configured for ÄKTA® and OligoPilot®
 
M045080 6-Tetrachloro-Fluorescein Phosphoramidite C46H54N3O10Cl4P configured for PerkinElmer
configured for Polygen