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Fast Deprotection 2′O-Methyl RNA Phosphoramidites

2′O-Methyl RNA is a nucleic acid analogue that is characterized by the exceptional hybridization properties that it imparts with complimentary DNA or RNA, as well as increased stability against enzymatic degradation compared to natural nucleic acids.

The unique combination of properties of 2′O-Methyl RNA has found widespread use in the fields of:
  • Diagnostic probes
  • Aptamer and ribozyme development
  • Mixed 2′O-Methyl-RNA/DNA antisense molecules

2′O-Methyl RNA nucleoside can be advantageously incorporated in nucleic acid probes with RNA or DNA for in-vivo or in-vitro applications to convey nuclease resistance.


Key features of 2′O-Methyl RNA Phosphoramidites
  • High yield of oligonucleotides
  • Compatible with DNA synthesis
  • Can be employed together with DNA or RNA phosphoramidites in the same synthesis to produce mixmer oligonucleotides
  • Purification and other downstream processing of fully modified 2′O-Methyl RNA oligonucleotides are simpler than in the case of RNA, as no special precautions are required to provide protection against nucleolytic degradation
  • Synthesis of 2′O-Methyl RNA oligonucleotides is similar to standard DNA synthesis, but requires an elongated coupling time (recommended is 6 minutes compared to 90 seconds for DNA monomers)

DMT-2′O-Methyl-rA(tac) Phosphoramidite

Product #

Structure

Description

Formula

Compatibility

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A212130 DMT-2′O-Methyl-rA(tac) Phosphoramidite C53H64N7O9P configured for ABI
 
A212100 DMT-2′O-Methyl-rA(tac) Phosphoramidite C53H64N7O9P    
A212150 DMT-2′O-Methyl-rA(tac) Phosphoramidite C53H64N7O9P configured for (ÄKTA® and OligoPilot®)
 
A212180 DMT-2′O-Methyl-rA(tac) Phosphoramidite C53H64N7O9P configured for PerkinElmer
configured for Polygen
 

DMT-2′O-Methyl-rC(tac) Phosphoramidite

Product #

Structure

Description

Formula

Compatibility

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C21218-HH DMT-2′O-Methyl-rC(tac) Phosphoramidite C52H64N5O10P configured for PerkinElmer 8900
configured for Polygen
pkg of 1 × 0.5 g (C212181-01)
C21213-HH DMT-2′O-Methyl-rC(tac) Phosphoramidite C52H64N5O10P configured for ABI
pkg of 1 × 0.5 g (C212131-01)
pkg of 1 × 1 g (C212161-01)
C212130 DMT-2′O-Methyl-rC(tac) Phosphoramidite C52H64N5O10P configured for ABI
 
C212180 DMT-2′O-Methyl-rC(tac) Phosphoramidite C52H64N5O10P configured for PerkinElmer
configured for Polygen
 
C212150 DMT-2′O-Methyl-rC(tac) Phosphoramidite C52H64N5O10P configured for (ÄKTA® and OligoPilot®)
 
C212100 DMT-2′O-Methyl-rC(tac) Phosphoramidite C52H64N5O10P    

DMT-2′O-Methyl-rG(tac) Phosphoramidite

Product #

Structure

Description

Formula

Compatibility

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G2121AO-HH DMT-2′O-Methyl-rG(tac) Phosphoramidite C53H64N7O10P configured for ÄKTA® and OligoPilot®
pkg of 1 × 10 g G212110-01
pkg of 1 × 5 g G212105-01
G21218-HH DMT-2′O-Methyl-rG(tac) Phosphoramidite C53H64N7O10P configured for PerkinElmer
configured for Polygen
pkg of 1 × 0.5 g G212181-01
G212130 DMT-2′O-Methyl-rG(tac) Phosphoramidite C53H64N7O10P configured for ABI
 
G212180 DMT-2′O-Methyl-rG(tac) Phosphoramidite C53H64N7O10P configured for PerkinElmer
configured for Polygen
 
G212150 DMT-2′O-Methyl-rG(tac) Phosphoramidite C53H64N7O10P configured for (ÄKTA® and OligoPilot®)
configured for ABI
 
G212100 DMT-2′O-Methyl-rG(tac) Phosphoramidite C53H64N7O10P    

DMT-2′O-Methyl-rU Phosphoramidite

Product #

Structure

Description

Formula

Compatibility

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U21118-HH DMT-2′O-Methyl-rU Phosphoramidite C40H49N4O9P configured for PerkinElmer 8900
configured for Polygen
pkg of 1 × 0.5 g (U211181-01)
U211100 DMT-2′O-Methyl-rU Phosphoramidite C40H49N4O9P    
U211130 DMT-2′O-Methyl-rU Phosphoramidite C40H49N4O9P configured for ABI
 
U211180 DMT-2′O-Methyl-rU Phosphoramidite C40H49N4O9P configured for PerkinElmer
configured for Polygen
 
U211150 DMT-2′O-Methyl-rU Phosphoramidite C40H49N4O9P configured for ÄKTA® and OligoPilot®