Standard Deprotected 2′O-Methyl RNA Phosphoramidites

2’O-Methyl RNA is a nucleic acid analogue that is characterized by the exceptional hybridization properties that it imparts with complimentary DNA or RNA, as well as increased stability against enzymatic degradation compared to natural nucleic acids.

The unique combination of properties of 2’O-Methyl RNA has found widespread use in the fields of:
  • Diagnostic probes
  • Aptamer and ribozyme development
  • Mixed 2’O-Methyl-RNA/DNA antisense molecules

2’O-Methyl RNA nucleoside can be advantageously incorporated in nucleic acid probes with RNA or DNA for in-vivo or in-vitro applications to convey nuclease resistance.


Key features of 2’O-Methyl RNA Phosphoramidites
  • High yield of oligonucleotides
  • Compatible with DNA synthesis
  • Can be employed together with DNA or RNA phosphoramidites in the same synthesis to produce mixmer oligonucleotides
  • Recommended deprotection conditions are 8 hours at 55°C using concentrated ammonia solution, or with AMA (concentrated ammonia/40% aqueous methylamine I/I, v/v) for 10 minutes at 65°C
  • Purification and other downstream processing of fully modified 2’O-Methyl RNA oligonucleotides are simpler than in the case of RNA, as no special precautions are required to provide protection against nucleolytic degradation
  • Synthesis of 2’O-Methyl RNA oligonucleotides is similar to standard DNA synthesis, but requires an elongated coupling time (recommended is 6 minutes compared to 90 seconds for DNA monomers)

DMT-2′O-Methyl-rA(bz) Phosphoramidite

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A211100 DMT-2′O-Methyl-rA(bz) Phosphoramidite C48H54N7O8P    
A211130 DMT-2′O-Methyl-rA(bz) Phosphoramidite C48H54N7O8P configured for ABI
 
A211180 DMT-2′O-Methyl-rA(bz) Phosphoramidite C48H54N7O8P configured for PerkinElmer
configured for Polygen
 
A211150 DMT-2′O-Methyl-rA(bz) Phosphoramidite C48H54N7O8P configured for (ÄKTA® and OligoPilot®)
configured for ABI
 

DMT-2′O-Methyl-rC(ac) Phosphoramidite

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C21310-HH DMT-2′O-Methyl-rC(ac) Phosphoramidite C42H52N5O9P   pkg of 1 × 100 g C213111-01
pkg of 1 × 500 g C213151-01
C213100 DMT-2′O-Methyl-rC(ac) Phosphoramidite C42H52N5O9P    
C213130 DMT-2′O-Methyl-rC(ac) Phosphoramidite C42H52N5O9P configured for ABI
 
C213180 DMT-2′O-Methyl-rC(ac) Phosphoramidite C42H52N5O9P configured for PerkinElmer
configured for Polygen
 

DMT-2′O-Methyl-rG(ib) Phosphoramidite

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G21118-HH DMT-2′O-Methyl-rG(ib) Phosphoramidite C45H56N7O9P configured for PerkinElmer 8900
configured for Polygen
pkg of 1 × 0.5 g (G211181-01)
G21110-HH DMT-2′O-Methyl-rG(ib) Phosphoramidite C45H56N7O9P   pkg of 1 × 100 g (G211111-01)
pkg of 1 × 500 g G211151-01
G211100 DMT-2′O-Methyl-rG(ib) Phosphoramidite C45H56N7O9P    
G211130 DMT-2′O-Methyl-rG(ib) Phosphoramidite C45H56N7O9P configured for ABI
 
G211180 DMT-2′O-Methyl-rG(ib) Phosphoramidite C45H56N7O9P configured for PerkinElmer
configured for Polygen
 
G211150 DMT-2′O-Methyl-rG(ib) Phosphoramidite C45H56N7O9P configured for (ÄKTA® and OligoPilot®)
configured for ABI
 

DMT-2′O-Methyl-rU Phosphoramidite

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U21118-HH DMT-2′O-Methyl-rU Phosphoramidite C40H49N4O9P configured for PerkinElmer 8900
configured for Polygen
pkg of 1 × 0.5 g (U211181-01)
U211100 DMT-2′O-Methyl-rU Phosphoramidite C40H49N4O9P    
U211130 DMT-2′O-Methyl-rU Phosphoramidite C40H49N4O9P configured for ABI
 
U211180 DMT-2′O-Methyl-rU Phosphoramidite C40H49N4O9P configured for PerkinElmer
configured for Polygen
 
U211150 DMT-2′O-Methyl-rU Phosphoramidite C40H49N4O9P configured for ÄKTA® and OligoPilot®
 
U211040 DMT-2′O-TBDMS-rU Phosphoramidite C45H61N4O9PSi configured for MerMade