Metabolic and Nutrition Research

Stable Isotope Labeled Products for Metabolic Research

Stable isotope labeled products serve as useful tools in the study of steady state and dynamic metabolomics. These compounds are ideal for studying metabolic flux, as they closely mimic endogenous natural abundance metabolites. Stable isotope tracer techniques are often paired with mass spectrometric methodology to give dynamic information regarding turnover rates and physiological regulation.1 Tracer methodology has routinely been used to study the biosynthesis and degradation of metabolites within healthy2 and diseased systems. While it is important to understand how a normal system operates, metabolic changes are intricately related to the development and progression of numerous diseases, including diabetes3 and cancer.4 These areas are heavily studied; however, the natural complexities surrounding the many overlapping biosynthetic pathways leave a diverse array of metabolic questions to be answered.

The team at MilliporeSIgma Stable Isotopes is uniquely prepared to provide products needed for all types of nutrition and metabolic research. This includes an extensive array of 13C, 15N, D and 18O labeled compounds, as well as products with multiple and varied labeling patterns. A significant portion of metabolic research involves in vivo studies, and for this reason, we also provide materials which have undergone extended quality testing. Many standard products are available that have undergone S&P (sterility and pyrogen) testing and by request, any product can be tested in this way. There are also several products available that meet the more rigorous testing requirements of the European and United States Pharmacopeias. We also operate full GMP space and can accommodate requests for products made in this environment.

Contact us at isosales@sial.com, +1-937-859-1808 or (800) 448-9760

References
1. Godin, J.-P.; Ross, A.B.; Rezzi, S.; Poussin, C.; Martin, F.-P.; Fuerholz,A.; Clérous, M.; Mermoud, A.-F.; Tornier, L.; Vera, F.A.; Pouteau, E.; Ramadan, Z.; Kochhar, S.; Fay,L.-B.; Anal. Chem. 2010, 82, 646
2. Kaddurah-Daouk, R.; Kristal, B.S.; Weinshilboum,R.M.; Annu. Rev. Pharmacol. Toxicol. 2008, 48, 653.
3. Fabbrini, E.; Magkos, F.; Mohamed, B.S.; Pietka, T.; Abumrad, N.A.; Patterson, B.W.; Okunade, A.; Klein, S.; PNAS, 2009, 106, 15430.
4. Lane, A.N.; Fan, T.W.-M.; Higashi, R.M.; Tan, J.; Bousamra, M.; Miller, D.M.; Exp. Mol. Pathol. 2009, 86, 165.

Literature
Stable Isotope Labeled Fatty Acids as Metabolic Tracers (J.E.Lambert, Ph.D. and E.J. Parks, Ph.D.)
Nutrition and Metabolic Studies (W.W. Wong, Ph.D.)
An Approach to Quantifying the Conversion of Carbohydrates to Fat in Humans (J.-M.Schwarz, Ph.D. and N.Bergeron, Ph.D.)
Bioactive Labeled Standards (Brochure)
Using stable isotopes in metabolic research with an emphasis on 13C, 15N or deuterated compounds offers a safe and reliable tool for metabolic profiling. Isotopic tracer and energy expenditure studies, coupled with the use of LC/MS, GC/MS and NMR, allows researchers to investigate specific pathways involving TCA cycle, glycolysis, fatty acids / amino acids / protein metabolism. Doubly labeled water also continues to be a valuable tool in energy expenditure and nutrition and metabolic studies. ISOTEC offers a wide range of products with high enrichment, purity and S&P testing to meet researchers′ needs.

Stable Isotope Home Page