Nucleotides

Stable Isotope Labeled Nucleic Acids for Biomolecular NMR

Advancements in NMR methodology, increased availability of larger, more powerful NMR instrumentation and the availability of stable isotope labeled nucleic acids have made the structure and function elucidation of nucleotide macromolecules a genuine reality. The use of labeled nucleic acids helps to overcome challenges such as extensive spectral crowding, increased resonance linewidths, and overall NMR signal degeneracy by providing the ability to enhance experimental sensitivity and spectral resolution. These enhancements help to increase the accuracy of solution structure determinations by facilitating the measurements of local and long-range distance restraints. 13C and/or 15N labeled nucleic acids have been employed study the links between RNA secondary structure and global conformation as well as determine dynamics in DNA flexibility. However, as the size of the macromolecules being studied increases, so do the challenges associated with resolution and relaxation rates. The use of deuterium has become the most attractive solution to combat these issues. Deuterium labeling is able to reduce linewidths, increase signal-to-noise ratios, and reduce chemical shift overlap by removing competing relaxation pathways.

ISOTEC Stable Isotopes offers an extensive array of labeled nucleic acids including our deuterated ribonucleotide triphosphates. All of our NTPs are suspended in 5 mM Tris-HCl buffer and are packaged in convenient 1 mg, 10 mg, and 25 mg quantities while our NMPs are supplied as a sodium salt powder.

Literature:
Products for Nucleic Acid Research

Deoxyribonucleotide monophosphates

Product #

Description

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648620 2′-Deoxyadenosine-13C10,15N5-monophosphate sodium salt 98 atom % 15N, 98 atom % 13C, 90% (CP)
    • 648612 2′-Deoxycytidine-13C9,15N3 5′-monophosphate sodium salt 98 atom % 13C, 98 atom % 15N, 90% (CP)
    • 648604 2′-Deoxyguanosine-13C10,15N5 5′-monophosphate sodium salt 98 atom % 15N, 98 atom % 13C, 90% (CP)
    • 648590 Thymidine-13C10, 15N2 5′-monophosphate sodium salt 98% 13C, 98 atom % 15N, 90% (CP)

    • Deoxyribonucleotide triphosphates

    Product #

    Description

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    646237 2′-Deoxyadenosine-13C10,15N5 5′-triphosphate sodium salt solution Supplied as sodium salt in 100 mM soln in H2O, 5 mM Tris HCl buffer, 98 atom % 15N, 98 atom % 13C, 90% (CP)
    • 646229 2′-Deoxycytidine-13C9,15N3 5′-triphosphate sodium salt solution Supplied as sodium salt in 100mM soln in H2O, 5mM Tris HCl buffer, 98 atom % 13C, 98 atom % 15N, 90% (CP)
    • 646210 2′-Deoxyguanosine-13C10,15N5 5′-triphosphate sodium salt solution Supplied as sodium salt in 100 mM soln in H2O, with 5 mM Tris buffer, 98 atom % 13C, 98 atom % 15N, 90% (CP)
    • 646202 Thymidine-13C10,15N2 5′-triphosphate sodium salt solution Supplied as sodium salt in 100 mM soln in H2O, with 5 mM Tris buffer, 98 atom % 13C, 98 atom % 15N, 90% (CP)

    • Ribonucleotide monophosphates

    Product #

    Description

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    662658 Adenosine-15N5-5′-monophosphate sodium salt 98 atom % 15N, 90% (CP)
    • 650676 Adenosine-13C10,15N5 5′-monophosphate sodium salt 98 atom % 15N, 98 atom % 13C, 90% (CP)
    • 662682 Cytidine-15N3-5′-monophosphate sodium salt 98 atom % 15N, 90% (CP)
    • 650692 Cytidine-13C9,15N3 5′-monophosphate sodium salt 98 atom % 13C, 98 atom % 15N, 90% (CP)
    • 662674 Guanosine-15N5-5′-monophosphate sodium salt 98 atom % 15N, 90% (CP)
    • 650684 Guanosine-13C10,15N5 5′-monophosphate sodium salt 98 atom % 13C, 98 atom % 15N, 90% (CP)
    • 662666 Uridine-15N2-5′-monophosphate sodium salt 98 atom % 15N, 90% (CP)
    • 651370 Uridine-13C9,15N2 5′-monophosphate sodium salt 98 atom % 15N, 98 atom % 13C, 90% (CP)

    • Ribonucleotide triphosphates

    Product #

    Description

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    738034 Adenosine-2,8-D2,1′,2′,3′,4′,5′,5′-D6 5′-triphosphate sodium salt solution Supplied as sodium salt in 100 mM soln in H2O, with 5 mM Tris buffer, 98 atom % D, 95% (CP)
    • 707783 Adenosine-15N5 5′-triphosphate sodium salt solution supplied as the sodium salt in 100 mM soln with H2O, with 5 mM Tris buffer, 98 atom % 15N, 95% (CP)
    • 710695 Adenosine-13C10 5′-triphosphate sodium salt solution supplied as sodium salt in 100 mM soln in H2O, with 5 mM Tris buffer, 98 atom % 13C, 95% (CP)
    • 645702 Adenosine-13C10,15N5 5′-triphosphate sodium salt solution Supplied as sodium salt in 100 mM soln in H2O, with 5 mM Tris buffer, 98 atom % 13C, 98 atom % 15N, 90% (CP)
    • 707759 Cytidine-15N3 5′-triphosphate sodium salt solution Supplied as sodium salt in 100 mM soln in H2O with 5 mM Tris buffer, 98 atom % 15N, 95% (CP)
    • 738042 Cytidine-5,6-D2,1′,2′,3′,4′,5′,5′-D6 5′-Triphosphate sodium salt solution Supplied as sodium salt in 100 mM solution in H2O, with 5 mM Tris buffer, 98 atom % D, 95% (CP)
    • 645699 Cytidine-13C9,15N3 5′-triphosphate sodium salt solution Supplied as sodium salt in 100 mM soln in H2O, with 5 mM Tris buffer, 98 atom % 13C, 98 atom % 15N, 90% (CP)
    • 711020 Cytidine-13C9 5′-triphosphate sodium salt solution supplied as sodium salt in 100 mM soln in H2O, with 5 mM Tris buffer, 98 atom % 13C, 95% (CP)
    • 707775 Guanosine-15N5 5′-triphosphate sodium salt solution supplied as the sodium salt in 100 mM soln in H2O, with 5 mM Tris buffer., 98 atom % 15N, 95% (CP)
    • 738069 Guanosine-8-D,1′,2′,3′,4′,5′,5′-D6 5′-Triphosphate sodium salt solution Supplied as sodium salt in 100 mM soln in H2O, with 5 mM Tris buffer, 98 atom % D, 95% (CP)
    • 710687 Guanosine-13C10 5′-triphosphate sodium salt solution supplied as sodium salt in 100 mM soln in H2O, with 5 mM Tris buffer, 98 atom % 13C, 95% (CP)
    • 645680 Guanosine-13C10,15N5 5′-triphosphate sodium salt solution supplied as sodium salt in 100mM soln in H2O, with 5mM Tris buffer, 98 atom % 13C, 98 atom % 15N, 90% (CP)
    • 707767 Uridine-15N2 5′-triphosphate sodium salt solution supplied as the sodium salt in 100 mM soln in H2O, with 5 mM Tris buffer, 98 atom % 15N, 95% (CP)
    • 738050 Uridine-5,6-D2,1′,2′,3′,4′,5′,5′-D6 5′-Triphosphate sodium salt solution Supplied as sodium salt in 100 mM soln in H2O, with 5 mM Tris buffer, 98 atom % D, 95% (CP)
    • 711012 Uridine-13C9 5′-triphosphate sodium salt solution supplied as sodium salt, in 100 mM soln in H2O, with 5 mM Tris buffer, 98 atom % 13C, 95% (CP)
    • 645672 Uridine-13C9,15N2 5′-triphosphate sodium salt solution supplied as sodium salt, in 100 mM soln in H2O, with 5 mM Tris buffer, 98 atom % 15N, 98 atom % 13C, 90% (CP)