Minimal Media Components

for Biomolecular NMR

The use of E.coli in the expression of isotopically enriched recombinant proteins for NMR studies has remained the most popular technique to date. The prevalence of this method is due to the availability of minimal media as the source of the isotopic label(s). Minimal media has been and continues to be the most economical method for obtaining isotopically enriched proteins and consists of M9 salts, glucose, and ammonium chloride or ammonium sulfate in either H2O or D2O. In certain methodology, such as auto-induction, the flexibility of minimal media is emphasized by using glycerol as the sole source for the carbon label instead of the predominantly utilized glucose.1 Minimal media has the capacity to label proteins in any combination of 13C, 15N, and/or D patterns and has been used in a broad range of research applications including brain chemistry2, bacterial modes of infection3, characterization of photocatalytic materials4, and childhood obesity5.

References
1 Studier, F.W. Protein Expr Purif. 2005. 41(1), 207-34
2 Patti, G.J.; Woo, H.; Yanes, O.; Shriver, L.; Thomas, D.;Uritboonthai, W.; Apon, J.V.; Steenwyk, R.; Manchester, M.; Siuzdak, G. Anal. Chem. 2010, 82, 121-128.
3 Wang, Yu.; Nordhues, B.A.; Zhong, D.; De Guzman, R.N. NMR Biochemistry. 2010, 49, 4220-4226.
4 Rockafellow, E.R.; Fang, X.; Trawyn, B.G.; Schmidt-Rohr, K.; Jenks, W.S. Chem. Mater. 2009, 21 (7), pp 1187-1197
5 Yzabek, S.N.; Buchner, D.A.; Geisinger, J.M.; Burrage, L.C.; Spiezio,S.H.; Zentner, G.E.; Hsieh, C.; Schacheri, P.C.; Croniger, C.M.; Nadeau, J.H. 2011 doi: 10.1101

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299251 Ammonium-15N chloride ≥98 atom % 15N, ≥99% (CP)
594091 Ammonium-15N,d4 deuteroxide solution ~3 N in D2O, 98 atom % D, 99 atom % 15N
488011 Ammonium-15N hydroxide solution ~3 N in H2O, 98 atom % 15N
299286 Ammonium-15N2 sulfate 98 atom % 15N
151882 Deuterium oxide 99.9 atom % D
435767 Deuterium oxide 99 atom % D
613428 Deuterium oxide 70 atom % D
756822 Deuterium oxide filtered, 99.8 atom % D
297046 D-Glucose-1-13C 99 atom % 13C
552003 D-Glucose-1,2,3,4,5,6,6-d7 97 atom % D
389374 D-Glucose-13C6 ≥99 atom % 13C, ≥99% (CP)
552151 D-Glucose-13C6,1,2,3,4,5,6,6-d7 97 atom % D, 99 atom % 13C
616338 D-Glucose-d12 97 atom % D
492639 Glycerol-1,3-13C2 99 atom % 13C
489476 Glycerol-13C3 99 atom % 13C
669024 Glycerol-13C3, d8 99 atom % 13C, 98 atom % D
447498 Glycerol-d8 ≥98 atom % D, ≥98% (CP)
490733 Sodium pyruvate-3-13C 99 atom % 13C