Stable isotope coded labels enable researchers to perform mass spectrometric- based proteomics studies in the absence of metabolic labeling. Labeling occurs by site-specific incorporation of stable isotope labeled tags at cysteine residues or the general labeling of amines and carboxyl groups in protein samples. These techniques are particularly useful for applications where metabolic labeling is impractical or undesirable such as clinical samples. Many methods for the chemical derivatization of proteins exist (1-3) offering researchers the ability to optimize stable isotope label incorporation based on target proteins. In addition to stable isotope labeled tags, the development of innovative matrixes and improvements in instrumentation greatly impact stable isotope usage in quantitative proteomics (4-5). ISOTEC has the high-quality isotopically enriched products needed for mass-spectrometry based quantitative proteomics. Consult with our R&D scientists to develop novel stable isotope labeled compounds for use in comparative proteomic applications.
1. Ong, S.E. and Mann, M. (2005) Mass spectrometry-based proteomincs turns quantitative. Nature Chemical Biology, 1, 252-262.
2. Julka, S. and Regnier, F. (2004) Quantification in Proteomics through Stable Isotope Coding: A Review. Journal of Proteome Research, 3, 350-363.
3. Julka, S. and Regnier, F. (2005) Recent Advancements in differential proteomics based on stable isotope coding. Briefings in Functional Genomics and Proteomics, 4(2), 158-177.
4. Matsuo, E., Toda, C., Watanobe, M., Ojima, N., Izumi, S., Tanaka, K., Tsunasawa, S., Nishimura, O. (2006) Selective detection of 2-nitrobenzenesulfenyl-labeled
peptides by matrix-assisted laser desorption/ionization-time of flight mass spectrometry using a novel matrix. Proteomics, 6, 2042-9.
5. Fenselau, C. (2007) A review of quantitative methods for proteomic studies. Journal of Chromatography B, 855, 14-20.