Acyl Coenzyme A Derivatives

Coenzyme A plays an important role in lipid biosynthesis, catabolic/oxidation processes and enzyme regulation. Coenzyme A is a key cofactor that aids in transferring acyl groups during enzymatic reactions. Researchers use isotopic tracer methods to study the kinetics of acyl coenzyme A activity. For example, ¹³C₁₆-palmitoyl coenzyme A was used to quantitate long chain acyl coenzyme A concentrations and enrichment.⁽¹⁾ Radiolabeled acyl coenzyme A is also used in developing assays to measure enzymatic activity. An article describes an efficient purification method for coenzyme A using radiolabeled ¹⁴C-acyl coenzyme A.⁽²⁾ In another instance, ¹³C-malonyl coenzyme A is used to measure the activity of malonyl coenzyme decarboxylase ⁽³⁾ in an effort to offer a safer, nonradioactive method.

Until now, researchers had to rely on synthesizing their desired ¹³C-labeled acyl coenzyme A compound or use radiolabeled ¹⁴C-coenzyme A to perform their experiments. With increased interest in isotopically labeled coenzyme A tracers, we now offer a range of short-, medium- and long chain ¹³C-acyl coenzyme A products for your convenience. As a leader in the stable isotope industry, we are the first to provide this unique range of ¹³C-acyl coenzyme A products with the highest isotopic enrichment and purity to our customers.

References:
1.Sun D, Cree MG, Wolfe RR. Quantification of the concentration and ¹³C tracer enrichment of long-chain fatty acyl-coenzyme A in muscle by liquid chromatography/mass spectrometry. Analytical Biochemistry 349 (2006) 87-95.
2. Minkler PE, Kerner J, Ingalls ST, Hoppel CL. Novel isolation procedure for short-, medium-, and long-chain acyl-coenzyme A esters from tissue. Analytical Biochemistry 376 (2008) 275-276.
3. Wang X, Stanley WC, Brunengraber H , Kasumov, T. Assay of the activity of malonyl-coenzyme A decarboxylase by gas chromatography-mass spectrometry. Analytical Biochemistry 363 (2007) 169-174.