Stable Isotope Labeled Acyl Coenzyme A Derivatives
Coenzyme A plays an important role in lipid biosynthesis, catabolic/oxidation processes and enzyme regulation. Coenzyme A is a key cofactor that aids in transferring acyl groups during enzymatic reactions. Researchers use isotopic tracer methods to study the kinetics of acyl coenzyme A activity. For example, 13C16-palmitoyl coenzyme A was used to quantitate long chain acyl coenzyme A concentrations and enrichment.(1) Radiolabeled acyl coenzyme A is also used in developing assays to measure enzymatic activity. An article describes an efficient purification method for coenzyme A using radiolabeled 14C-acyl coenzyme A.(2) In another instance, 13C-malonyl coenzyme A is used to measure the activity of malonyl coenzyme decarboxylase (3) in an effort to offer a safer, nonradioactive method.
Until now, researchers had to rely on synthesizing their desired 13C-labeled acyl coenzyme A compound or use radiolabeled 14C-coenzyme A to perform their experiments. With increased interest in isotopically labeled coenzyme A tracers, we now offer a range of short-, medium- and long chain 13C-acyl coenzyme A products for your convenience. As a leader in the stable isotope industry, we are the first to provide this unique range of 13C-acyl coenzyme A products with the highest isotopic enrichment and purity to our customers.
References: 1.Sun D, Cree MG, Wolfe RR. Quantification of the concentration and 13C tracer enrichment of long-chain fatty acyl-coenzyme A in muscle by liquid chromatography/mass spectrometry. Analytical Biochemistry 349 (2006) 87-95. 2. Minkler PE, Kerner J, Ingalls ST, Hoppel CL. Novel isolation procedure for short-, medium-, and long-chain acyl-coenzyme A esters from tissue. Analytical Biochemistry 376 (2008) 275-276. 3. Wang X, Stanley WC, Brunengraber H , Kasumov, T. Assay of the activity of malonyl-coenzyme A decarboxylase by gas chromatography-mass spectrometry. Analytical Biochemistry 363 (2007) 169-174.