HPLC

BIO PolyMA SCX

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Discovery BIO PolyMA-SCX
Polymethacrylate polymer-based strong cation-exchange HPLC column
 
Discovery BIO PolyMA-SCX polymer-based cation-exchange particles have discriminating hydrophilic surface chemistry making them ideally suited for separating proteins, peptides, and other biotechnology-derived products.

Choose Discovery BIO PolyMA-SCX for cation-exchange separations. Generally run at a pH less than the protein's pI (isoelectric point) - usually below pH 7.
 
Particle:   Polymethacrylate with hydrophilic coating
Functional Group:   Sulfopropyl (SP)
Counterion (as supplied):   Na+
Particle Shape:   Spherical, monodispersed
Particle Size:   5µm
Pore Size:   1000Å
Coverage:   0.3meq/g
pH Range:   1 to 13
Temperature Range:   4°C to 50°C
 
Solutions for Protein and Peptide Separation Challenges
 

Excellent separations of protein isoforms

High resolution at low sample load

Quantitative recovery - a hydrophilic surface eliminates protein adsorption

High efficiency

Wide pH range


Here's the
Proof
 
EFFICIENT CATION-EXCHANGE SEPARATIONS
 
Discovery BIO PolyMA-SCX typically gives better resolution than competitive polymeric ion-exchange materials.
 
Comparison of Efficiency: Cytochrome c Variants
Columns:   Discovery BIO PolyMA-SCX, 5cm x 4.6mm, 5µm (Cat. No. 59601-U) and Competitive polymeric-SCX columns of similar dimensions
Mobile Phase:   (A) 50mM MOPS/KOH, pH 7.0;
(B) 50mM MOPS/KOH, 0.5M KCl, pH 7.0
Flow:   3.01cm/min (flow rates appear in Figure)
Temp.:   35°C
Detection:   UV, 280nm
Sample:   10µg each variant
Gradient Profile:   0.6% B per minute. See Figure for details.
     
1.   Cow cytochrome c
2.   Horse cytochrome c
3.   Rabbit cytochrome c
 
 
BALANCE OF ION-EXCHANGE CAPACITY AND PROTEIN RECOVERY
 
Although capacity is dependent on the protein under study and operating conditions, generally between 20 and 50mg of total protein can be loaded onto the Discovery BIO PolyMA-SCX columns.
 
Ion-exchange Capacity
Column Protein mg per mL of column volume mg per column
Discovery BIO PolyMA-SCX Lysozyme 40mg 30mg
 
Loading studies were run at 240cm/hr (0.67mL/min) using 0-1M NaCl salt gradients in 20mM sodium phosphate, pH 7. Protein concentration was 2mg/mL in starting mobile phase.
 
Protein (Fibrinogen) Recovery  
  Cytochrome C Recovery
Column 1st Injection 2nd Injection 5th Injection
Discovery BIO PolyMA-SCX (5µm, 5cm x 4.6mm) 99.5% 98.6% 100.2%
Competitive polymethacrylic SP column (10µm, 7.5cm x 7.5mm) 59.0% 76.1% 90.6%
 
Mobile phase: Gradient of 0 - 1M NaCl in 20mM citrate (pH 4) over 30 minutes. Flow Rate: 1mL/min. Ambient temperature. Sample: 40µg cytochrome c in 20µL starting mobile phase.

COLUMN STABILITY AND LIFETIME
 
Column stability is important from quality and economic standpoints. Stable columns give reliable results. Stable columns cost less per injection and cause less system down-time. Results obtained on Discovery BIO PolyMA-SCX will be reproducible injection after injection because of exceptional column stability. In the figure below, Discovery BIO PolyMA-SCX columns are shown to give stable retention of three proteins after 500 injections, with no sign of deterioration.
 
Stability of Discovery BIO PolyMA-SCX Columns
Column:   Discovery BIO PolyMA-SCX, 5cm x 4.6mm, 5µm (Cat. No. 59601-U)
Mobile Phase:   (A) 20mM sodium phosphate, pH 7.0
(B) 20mM sodium phosphate, 0.5M NaCl, pH 7.0
Flow:   0.5mL/min
Temp.:   25°C
Detection:   UV, 280nm
Sample:   Ribonuclease A (10µg), a-chymotrypsinogen (5µg), cytochrome c (5µg)
Gradient Profile:  
time (min.) %A %B
0 95 5
15 0 100

 


Ordering Information
Discovery BIO PolyMA-SCX HPLC Column
Particle Size (µm) ID (mm) Length (cm) Cat. No.
5 4.6 5 59601-U
 
Column Protection: We recommend filtering samples and using an in-line filter to protect Discovery BIO PolyMA-SCX columns.
Description Cat. No.
In-line filter, 0.5 micron frits Z227323

view the pdf documentTo view the Care and Use Guide for Discovery BIO PolyMA-SCX (10KB PDF), click the pdf icon.

How to Contact Supelco
 
USA only:
Phone: 800-247-6628
  or 814-359-3441
fax: 800-325-5052
email: techservice@sial.com
 
Outside the USA: click here

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