HPLC

DiscoveryBIO Wide Pore C18

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Ordering Information


Discovery BIO Wide Pore C18
HPLC Columns and Capillaries
 
Bonded Phase:   Covalently-bonded octadecylsilane (C18), endcapped
Silica:   Spherical, 3µm, 5µm and 10µm diameters, 300Å pore diameter,
    high purity (<10ppm metals)
Column IDs:   0.18mm to 21.2mm
 
Solutions for Protein and Peptide Separation Challenges
 

Proteomics and biotherapeutics: high resolution of synthetic peptides and peptide maps

LC/MS; no-bleed, capillary dimensions, and pH stability

Peptide purification: Scalable from analytical to preparative

Reproducible separations batch-to-batch and column-to-column


Here's the
Proof
 
MATCHED SELECTIVITY FROM ANALYTICAL TO PREP
 

Column: Discovery BIO Wide Pore C18, 15cm x 4.6mm, 3µm; 15cm x 4.6mm, 5µm; 15cm x 10mm, 10µm, Mobile Phase: (A) 80:20, (0.1% TFA in H2O) : (0.1%TFA in MeCN), (B) 66:34, (0.1% TFA in H2O) : (0.1%TFA in MeCN), Flow Rate: 6.02cm/min, Temp: 30°C, Detection: 215nm, Sample: Peptide Mix (Sigma Cat. No. P2693)

 

Column Parameters & Run Conditions:

Column

CV(mL) Injection(µL) Flow(mL/min)

15cm x 4.6mm, 3µm

1.64 5.0 1.00

15cm x 4.6mm, 5µm

1.71 5.2 1.00

15cm x 10mm, 10µm

8.01 24.5 4.73
 

Gradient:

CV %A %B
0 100 0
2 100 0
9 0 100
g001511_13

PEPTIDE MAP OF
CARBOXYMETHYLATED APOHEMOGLOBIN TRYPTIC DIGEST
 

Column: Discovery BIO Wide Pore C18, 15cm x 4.6mm, 5µm; Mobile Phase: (A) 95:5, (0.1% TFA in H2O):(0.1% TFA in MeCN); (B) 50:50, (0.1% TFA in H2O):(0.1% TFA in MeCN); Flow Rate: 1mL/min; Temp: 30°C; Detection: 215nm; Injection: 50µL; Sample: caboxymethylated apohemoglobin tryptic digest; Gradient: 0-100% B in 65 min

g001510

STABILITY OF DISCOVERY BIO WIDE PORE C18
AT ALKALINE pH
 

Alkaline pH Stability
Retention times of the sample components remained constant after 29,000 bed volumes were passed through the column.

Column: Discovery BIO Wide Pore C18, 5cm x 4.6mm, 5µm; Mobile Phase: 25mM potassium phosphate, pH 8 : MeOH (95:5); Flow Rate: 2mL/min; Temp: 35°C; Detection: 254nm

g001520


UNDETECTABLE LC/MS BLEED
 

LC/MS Bleed Analysis on Discovery BIO Wide Pore C18

Column: Discovery BIO Wide Pore C18, 15cm x 4.6mm, 3µm; Mobile Phase: (A) 0.1% TFA in H2O, (B) 0.1% TFA in MeOH; Flow Rate: 1mL/min; Temp: 30°C; Gradient: 0-100% B in 15 min, hold for 5 min, then back to 0% B for 10 min

 

No TIC baseline rise relative to blank run (gradient run w/o column)

g001522

g001523


LOT TO LOT REPRODUCIBILITY OF DISCOVERY BIO WIDE PORE C18
 

Column: Discovery BIO Wide Pore C18, 15cm x 4.6mm, 5µm; Mobile Phase: 80% 10mM ammonium acetate, pH 6.8 : 20% MeOH; Flow Rate: 1mL/min; Temp: 35°C; Detection: 254nm; Injection: 5µL

1. Uracil (8µg/mL)
2. Procainamide (30µg/mL)
3. Sorbic acid (4µg/mL)
4. Pyridine (100µg/mL)
5. Caffeine (40µg/mL)
6. Phenol (240µg/mL)

g001514_6

Ordering Information
3µm Discovery BIO Wide Pore C18 HPLC Columns
ID(mm) Length(cm) Cat. No.
0.18 5 65603-U
0.18 10 65604-U
0.32 5 65526-U
0.32 10 65527-U
0.5 5 65517-U
0.5 10 65518-U
1.0 5 65504-U
1.0 10 65506-U
2.1 5 567200-U
2.1 10 567201-U
2.1 15 567202-U
4.6 5 567203-U
4.6 10 567204-U
4.6 15 567205-U
5µm Discovery BIO Wide Pore C18 HPLC Columns
ID(mm) Length(cm) Cat. No.
0.18 5 65606-U
0.18 10 65607-U
0.18 15 65608-U
0.32 15 65529-U
0.5 15 65519-U
1.0 15 65508-U
1.0 25 65509-U
2.1 5 568200-U
2.1 10 568201-U
2.1 15 568202-U
2.1 25 568203-U
4.0 5 568210-U
4.0 10 568211-U
4.0 15 568212-U
4.0 25 568213-U
4.6 5 568220-U
4.6 10 568221-U
4.6 15 568222-U
4.6 25 568223-U
10.0 25 568230-U
10µm Discovery BIO Wide Pore C18 HPLC Columns
ID(mm) Length(cm) Cat. No.
4.6 25 567206-U
10.0 5 567207-U
10.0 15 567208-U
10.0 25 567209-U
21.2 5 567210-U
21.2 15 567211-U
21.2 25 567212-U
2cm Supelguard Cartridges with Discovery BIO C18 Packings
  Description 2.1mm ID 4.0mm ID
  3µm Particle Size
 
  2 Pack
567270-U 567272-U
 
  Kit triangle
567271-U 567273-U
  5µm Particle Size
 
  2 Pack
568270-U 568272-U
 
  Kit triangle
568271-U 568273-U
  For 4.0mm ID or 4.6mm ID analytical columns
triangle Kit includes a holder and one replaceable cartridge.
1cm Supelguard Cartridges with Discovery BIO C18 Packings
  Description 10mm ID
  10µm Particle Size 567282-U