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HPLC

Discovery Zr CarbonC18 HPLC columns

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Ordering Information

Discovery Zr-CarbonC18 HPLC columns
 
Discovery Zr-CarbonC18 consists of spherical, porous carbon-clad zirconia particles covalently modified with octadecyl (C18) groups. It is intended for the reversed-phase separation of acidic and neutral compounds. It complements the selectivity offering of the other zirconia-, silica-, and polymer-based Discovery phases, and allows the use of the full range of mobile phase pH from pH 1 to 14.
 
Particle:   Zirconia (ZrO2)
Bonded Phase:   Octadecyl(C18)-bonded Carbon
Particle Shape:   Spherical
Particle Size:   3 and 5µm
Pore Size:   300Å
Surface Area:   30m2/g
pH Range:   1 to 14
Temperature Range:   up to 100°C in standard hardware (up to 200°C in custom all-steel hardware)
 
Unique retention and selectivity for method development at pH and temperature extremes
 

Very different selectivity compared to C18-silica

Resistant to phase hydrolysis

pH stable from 1-14

Thermally stable up to 200°C


Here's the
Proof
 
RAPID LOW pH AND HIGH TEMPERATURE SEPARATIONS
 
Discovery Zr-CarbonC18 has the partitioning mechanism of C18, but because it is bonded to a highly inert, carbonaceous support, it is immune to pH and temperature extremes. The examples of the acidic non-steroidal anti-inflammatory compounds and in the figures below run at pH 1.75 and 80°C on Discovery Zr-CarbonC18 demonstrates the extreme applicability of this phase.
 
figure I
 
RAPID HIGH pH AND HIGH TEMPERATURE SEPARATIONS
 
Increasing the temperature can greatly reduce the analysis time. The thermal stability of all Discovery Zr phases allows temperatures up to 100°C and higher with special hardware. The separation of ß-blockers on Discovery Zr-CarbonC18 at 80°C in less than 1 minute is shown in the figure below.
 
figure J
 
PARTIONING AND SHAPE-SELECTIVE RETENTION MECHANISMS
 
The separation of positional isomers of a proprietary sulfonamide drug is shown in the figure below. Here the parent compound is easily distinguished between its three corresponding positional isomers.
 
figure K

Ordering Information
Phase ID (mm) Length (cm) Cat. No.
3µm Discovery Zr-CarbonC18 Columns
  2.1 5 65701-U
  2.1 7.5 65702-U
  2.1 15 65703-U
  4.6 5 65704-U
  4.6 7.5 65705-U
  4.6 15 65706-U
5µm Discovery Zr-CarbonC18 Columns
  2.1 5 65707-U
  2.1 15 65708-U
  4.6 5 65710-U
  4.6 15 65711-U
 

Description 2.1mm ID 4.0mm ID
1cm Supelguard Cartridges with Discovery Zr-CarbonC18 Packings
  3µm Particle Size
 
  2 Pack
65802-U 65804-U
 
  Kit triangle
65801-U 65803-U
  5µm Particle Size
 
  2 Pack
65806-U 65808-U
 
  Kit triangle
65805-U 65807-U
 
For 4.0mm ID or 4.6mm ID analytical columns
Kit includes a holder and one replaceable cartridge.
   

For additional information, click the pdf icons below.
view the pdf document Bulletin 932 - Discovery Zr: Method Development Guidlines (48 KB)
view the pdf document Care and Use Guide for Discovery Zr-CarbonC18 (15 KB)

How to Contact Supelco
 
In USA:
Phone: 800-247-6628
  or 814-359-3441
fax: 800-325-5052
email: techservice@sial.com
 
Outside the USA: click here

TRADEMARKS
Discovery, and Supelguard are trademarks of Sigma-Aldrich Co.