bio-spme


Bio-SPME Video
BioSPME offers an innovative approach for biological microsampling and sample preparation, while enabling the direct measurement of free analyte fraction. The BioSPME platform enhances traditional LC/MS applications while driving the future for direct MS analysis.

This technology allows for the selective extraction of a broad range of analytes from biological samples while repelling unwanted macromolecules and matrix. We currently offer C18 and PDMS/DVB coated fibers and tips for a wide range of applications. More phases coming soon!


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Technical Resources


Feature Benefit
Method simplicity 1-step sampling and sample preparation combined reduces cost and time
Automation Friendly 96-well format for high throughput and decreased human error
Microsampling technique Perfect for small and precious samples
Direct Sampling No sample pretreatment necessary
Fiber selectivity Allows for direct measurement of free analyte fraction
Small desorption volumes   Increases sample concentration, enabling detection of less prevalent analytes

 


Fiber probe

96-tip array

 

Phase Chemistry Application
C18 For extraction of polar and nonpolar analytes from aqueous samples
PDMS/DVB For extraction of moderately polar semivolatile or volatile analytes from aqueous samples or headspace

 

Detection Levels 5 to 10 times higher compared to Dried Blood Spots (DBS)
Figure 1. Analytes Detected after Extraction with BioSPME and DBS Methods

This improvement in response from BioSPME comes primarily from the concentrating ability of the fiber coating, but also from the small desorption volumes needed to displace the analytes from the fibers. In contrast, the relatively small portion of sample obtained from the 3 mm punch of the DBS card and the large desorption volume required to fully cover the punch, limit the amount of sample that can be detected by the analytical method without evaporation to concentrate the sample. The result is analyte detection levels that are five- to ten-times higher using the SPME LC Tips compared to the DBS card.
 
Over 4x Reduction in Analysis Time Compared To Thermo RED (Rapid Equilibrium Dialysis)

BioSPME was compared Thermo RED technique for Drug Protein Binding Studies. The BioSPME protocol was approximately 60 minutes, whereas the Thermo RED protocol took over 4 hours. As shown here, BioSPME delivers speed and simplicity without compromising sensitivity. To see the procedure and get more info., view poster Rapid Determination of Drug Protein Binding Affinity using Solid Phase Microextraction.

Technical Resources
Description Type Id.
Product Literature
BioSPME Fibers and Probes Brochure T413047
SPME LC Tips Data Sheet T714005
SPME LC Fiber Probes Data Sheet T710118
Webinars
Biocompatible Solid Phase Microextraction (BioSPME): A New Tool for the Clinical Chemist
Solid Phase Microextraction: Sketching the Future of Microsampling in Bioanalysis; Sheelan Ahmad; Senior Scientist; GlaxoSmithKline R&D and the University of Hertfordshire
Basic Principles of Solid Phase Microextraction (SPME) Method Development
Articles
Rapid Determination of Protein Binding Affinity Using Solid Phase Microextraction
Direct Analysis in Real Time (DART®)-MS Analysis of Fentanyl and Related Analogues from Saliva Using Biocompatible Solid Phase Microextraction (BioSPME)
(DART®)-MS Screening Method for Methamphetamine and Amphetamine Using Biocompatible Solid Phase Microextraction (BioSPME)
Bioanalytical Microsampling using Biocompatible SPME and Benefits over Dried Blood Spots
Utilizing Novel SPME LC Tips for Forensic Screening of Drugs of Abuse in Urine Samples
Biocompatible SPME Devices for Bioanalysis and in vivo Sampling
Investigation of Bio-SPME Technology for the Enrichment of Illicit “Bath Salts” Cathinone Compounds from Biological Samples T413032H
C18 SPME Fiber Probes for LC Applications
Journal References
Biocompatible Solid-Phase Microextraction Nanoelectrospray Ionization: An Unexploited Tool in Bioanalysis. Anal Chem. 2016 Jan 19;88(2):1259-65. doi: 10.1021/acs.analchem.5b03668. Epub 2015 Dec 29. Gómez-Ríos GA, Reyes-Garcés N, Bojko B, Pawliszyn J.
Direct ionization of solid-phase microextraction fibers for quantitative drug bioanalysis: from peripheral circulation to mass spectrometry detection. Anal Chem. 2015 Jan 6;87(1):754-9. doi: 10.1021/ac503706n. Epub 2014 Dec 15. Ahmad S1, Tucker M, Spooner N, Murnane D, Gerhard U.
Coupling desorption electrospray ionization with solid-phase microextraction for screening and quantitative analysis of drugs in urine. Kennedy JH, Aurand C, Shirey R, Laughlin BC, Wiseman JM. Anal Chem. 2010 Sep 1;82(17):7502-8. doi: 10.1021/ac101295g.
Analytical methods used in conjunction with solid-phase microextraction: a review of recent bioanalytical applications. Bioanalysis. 2009 Sep;1(6):1081-102. doi: 10.4155/bio.09.88. Musteata ML, Musteata FM.

 

Products
Description Cat. No.
SPME-LC Fiber Needle Probe, functional group C18,package of 5 probes
57281-U
SPME-LC Pipette Tips, functional group C18, 96-tip array 57234-U
SPME-LC Pipette Tips, mixed mode PDMS/DVB, 96-tip array 57248-U
IonSense® PDMS/DVB SPE-it Tips, 96-tip array 57249-U
IonSense® C18 SPE-it Tips pkg of 96 ea 57264-U

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  • IonSense is a registered trademark of IonSense, Inc.