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Bioconjugation

Bioconjugation

Bioconjugation involves ligating molecules together in which at least one of the reactants is a biomolecule, often an antibody, protein, or oligonucleotide. Applications include attaching fluorescent probes to antibodies for flow cytometry and microscopy imaging, attaching antibodies to beads for immunoprecipitations, attaching antibodies to drugs for therapeutic development, and crosslinking of proteins to detect their biological interactions. For an easier workflow with immunoprecipitations we offer a liquid coating from Anteo that can be used to rapidly provide a noncovalent high-affinity attachment of antibodies to beads.

Often the bioconjugation is done using crosslinkers which can be homobifunctional with each end having the same reactive chemistry or heterobifunctional with the ends having two different reactive chemistries. Our crosslinkers contain diverse functional groups such as NHS esters to react with amines. For reaction with sulfhydryls we offer traditional maleimide coupling or the improvements of APN chemistry. Some crosslinkers have click chemistry groups on one end which can allow attachment to a molecule with a complementary click chemistry group. Crosslinkers are available with a variety of lengths and solubilities and may include cleavability.