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Chromatography Column Sizing Chart

What size column do I need?

Use this handy chart to determine what size column is required and estimate the typical fraction and sample size.

Column Size (mL) Fraction Size (mL) Sample size
Filtration Separation
200 10 5 1-2
400 20 10 3-5
600 30 20 5-8
100 50 30 10
2000 100 60 20-30
4000 150-200 100 50
8000 250-400 200 100
20,000 500-750 300 150-200