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Glassware Catalog

Chromatography, LC - Sigma-Aldrich Glassware Catalog

 

HPLC  •  LC  •  TLC

 

LC Chromatography

On this page:
Size Chart Flash-chromatography assembilies with threaded S.T. JointsFlash-chromatography assemblies with ball jointsGravity columnsJacketed chromatography columnsAccessories


Size Chart


Flash chromatography is useful for rapid, preparative separationswith moderate resolutions. As first described in J. Org. Chem. 1978, 43, 2923, it is commonly performed with silica gel although the use of reversed phase packings has become popular in recent years.

Column size
(mL)
Fraction size
(mL)
Sample size (g)
Filtration
Sample size (g)
Separation
,200 10 5 1 - 2
400 20 10 3 - 5
600 30 20 5 - 8
1,000 50 30 10
2,000 100 60 20 - 30
4,000 150 - 200 100 50
8,000 250 - 400 200 100
20,000 500 - 750 300 150 - 200

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Flash-chromatography assembilies with threaded S.T. Joints

 

Consists of column and flow controller.
See the Air-Sensitive section for a description of the threaded S.T. joint.

Cap (mL) S.T. Joints Standard assembly
Catalog Number
Assembly with fritted disc (porosity C)
Catalog Number
100 24/40 Z14,735-4 Z41,663-0
100 29/32 Z20,289-4 Z41,670-3
200 24/40 Z14,736-2 Z41,664-9
200 29/32 Z20,290-8 Z41,671-1
400 24/40 Z14,737-0 Z41,665-7
400 29/32 Z20,291-6 Z41,673-8
600 24/40 Z14,738-9 Z41,666-5
600 29/32 Z20,292-4 Z41,674-6
1,000 45/40 Z20,243-6 Z41,667-3
1,000 45/50 Z14,739-7 Z41,675-4
2,000 45/40 Z20,245-2 Z41,668-1
2,000 45/50 Z14,740-0 Z41,676-2



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Solvent reservoirs

Columns can accommodate all reservoirs with same joint size.

Cap (mL) S.T. Joints Standard assembly
Catalog Number
100 24/40 Z14,741-9
100 29/32 Z20,293-2
250 24/40 Z14,742-7
250 29/32 Z20,294-0
500 24/40 Z14,743-5
500 29/32 Z20,295-9
1,000 45/40 Z20,244-4
1,000 45/50 Z14,744-3
2,000 45/40 Z20,246-0
2,000 45/50 Z14,745-1

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Replacement Parts

Cap (mL) S.T. Joints O.d. x L (mm) Standard columns
Catalog Number
Fritted columns
Catalog Number
100 24/40 19 x 813 Z14,747-8 Z41,650-9
100 29/32 19 x 813 Z20,297-5 Z41,657-6
200 24/40 25 x 584 Z14,748-6 Z41,651-7
200 29/32 25 x 584 Z20,298-3 Z41,658-4
400 24/40 38 x 560 Z14,749-4 Z41,652-5
400 29/32 38 x 560 Z20,299-1 Z41,659-2
600 24/40 44 x 572 Z14,750-8 Z41,653-3
600 29/32 44 x 572 Z20,300-9 Z41,660-6
1,000 45/40 57 x 508 Z20,247-9 Z41,654-1
1,000 45/50 57 x 508 Z14,751-6 Z41,661-4
2,000 45/40 76 x 560 Z20,249-5 Z41,656-8
2,000 45/50 76 x 560 Z14,752-4 Z41,662-2

S.T. Joint Flow controllers
Catalog Number
Screw-caps
Catalog Number
Viton O-rings
Catalog Number
24/40 Z14,753-2 Z14,755-9 Z14,757-5
29/32 Z20,301-7 Z20,302-5 Z20,303-3
45/40 Z20,248-7 Z14,756-7 Z14,758-3
45/50 Z14,754-0 Z14,756-7 Z14,758-3


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Flash-chromatography assemblies with ball joints


Consists of column, flow controller, and pinch clamp.
Cap (mL) Ball Joints Standard assembly
Catalog Number
Assembly with frtted disc
(porosity C)

Catalog Number
100 28/12 Z10,409-4 Z41,687-8
100 29/15 Z20,229-0 Z41,688-6
200 50/30 Z10,410-8 Z41,689-4
200 51/30 Z20,233-9 Z41,690-8
400 50/30 Z10,411-6 Z41,691-6
400 51/30 Z20,239-8 Z41,692-4
600 65/40 Z10,412-4 Z41,693-2
1,000 75/50 Z10,413-2 Z41,694-0
2,000 75/50 Z11,725-0 Z41,695-9


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Solvent reservoirs

Use with above columns. Requires second pinch clamp; see below.

Cap (mL) Ball Joints Catalog Number
100 28/12 Z12,123-1
100 29/15 Z20,230-4
250 50/30 Z12,125-8
250 51/30 Z20,234-7
500 50/30 Z12,126-6
500 51/30 Z20,240-1
500 65/40 Z12,127-4
1,000 75/50 Z12,128-2
2,000 75/50 Z12,129-0


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Replacement Parts

Cap (mL) Ball Joints O.d. x L (mm) Standard columns
Catalog Number
Fritted columns
Catalog Number
100 28/12 19 x 813 Z10,697-6 Z41,677-0
100 29/15 19 x 813 Z20,232-0 Z41,678-9
200 50/30 25 x 584 Z10,698-4 Z41,679-7
200 51/30 25 x 584 Z20,236-3 Z41,680-0
400 50/30 38 x 560 Z10,699-2 Z41,681-9
400 51/30 38 x 560 Z20,242-8 Z41,682-7
600 65/40 44 x 572 Z10,701-8 Z41,683-5
1,000 75/50 57 x 508 Z10,702-6 Z41,684-3
2,000 75/50 76 x 560 Z11,746-3 Z41,685-1

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Ball Joint Flow controllers
Catalog Number
Pinch Clamp
Catalog Number
28/12 Z10,703-4 Z15,651-5
29/15 Z20,400-5 Z20,231-2
50/30 Z10,704-2 Z15,652-3
51/30 Z20,237-1 Z20,235-5
65/40 Z10,706-9 Z15,653-1
75/50 Z10,707-7 Z10,711-5


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Gravity columns


With 4mm PTFE stopcock. Has no frituses glass-wool plug and sand. Use with coarse silica gel (e.g.), Cat. No. 24,217-9) for fast, general-purpose purification.

L (cm) S.T. Joint I.d. x o.d. (mm) Catalog Number
Short, ca. 33 24/40 24 x 28 Z16,396-1
Long, ca. 70 24/40 24 x 28 Z16,398-8
Long, ca. 81 - 40 x 45 Z16,400-3

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Jacketed chromatography columns

S.T. 24/40 joint, gravity fed. A slight nitrogen pressure may be applied using an inlet adapter. Recommended packing: silica gel 923 (Aldrich Cat. No. 21,447-7).



Column
cap. (mL)
PTFE
stopcock
Reservoir
cap. (mL)
Max.
packing (g)
Catalog
Number
400 2mm 500 250 Z17,477-7
700 4mm 1,000 450 Z17,478-5

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Accessories

Solvent-pouring funnel

Unique, closed-end glass funnel has 2mm diam. holes in the stem.
Size: top 7.62cm, stem 6.35 L x 2.2cm o.d.

Z20,275-4


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Chromatography funnels

Use for solvent addition in flash chromatography. Unbreakable PP.

Size (mL) Top o.d./
stem L (mm)
Catalog Number
60
168/20 Z40,069-6
120
188/22 Z40,071-8
240
108/38 Z40,072-6
960
168/50 Z40,073-4
960/long stem
168/70 Z40,074-2
Set of one of each of the above
Z40,075-0







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Stationary Phases for Column Chromatography


Activated aluminum oxide (alumina)

58Å, Brockmann I, activated, for column chromatography, surface area 155m2/g, 150 mesh

Grade pH of aqueous suspension Catalog Number
Standard 4.5+/-0.5 19,996-6
Weakly acidic 6.0 26,774-0
Basic 9.5+/-0.5 19,944-3








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Silica gel

60Å, for column chromatography, surface area 500m2/g, pore volume 0.75cm3/g

Mesh size Catalog Number
70 - 270 28,861-6
130 - 270 28,860-8
200 - 400 28,859-4

Please see the Aldrich Handbook for more listings of stationary phases.

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