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Glassware Catalog

TLC -Sigma-Aldrich Glassware Catalog


HPLC  •  LC  •  TLC

TLC Chromatography

On this page:
Plates
AccessoriesTLC development chambersTLC developing tanksALDRICH CHROMATOGRAPHY SPRAYERSKimble micro capillary pipettes

Sigma-Aldrich TLC Plates

Thin-layer chromatography (TLC) plates for adsorption, partition, and ion exchange techniques. Six media choices with glass, polyester, and aluminum backings. Silica-based adsorbent layers in Sigma-Aldrich TLC plates incorporate a polymeric binder; highly purified silica gel plates incorporate a gypsum/polymer binder.

  • Silica gel - for weakly to strongly polar separations
  • High purity silica gel - acid washed for separation of aflatoxins
  • Cellulose - for partition chromatography
  • Cellulose PEI - for separation of weak anions (amino acids, peptides)
  • Aluminum oxide - basic alumina, for separation of hormones and antibiotics
  • Chiral silica - for separation of optically active isomers by ligand exchange

Adsorbent layer
Plate material
Plate dimensions
(cm x cm)
Fluorescent
indicator
Catalog
Number
Layer thickness: 200 mm, particle size <60 mm, pore size 60Å
Aluminum oxide
polyester
20 x 20
Y
Aluminum oxide
polyester
20 x 20
-
 
Layer thickness: 250 mm, particle size 2 to 20 mm
Cellulose
glass
10 x 20
-
Cellulose
glass
10 x 20
Y
Cellulose
glass
20 x 20
-
Cellulose
glass
20 x 20
Y
 
Layer thickness: 100 mm, particle size 2 to 20 mm
Cellulose
polyester
20 x 20
-
Cellulose
polyester
20 x 20
Y
Cellulose, PEI*
polyester
20 x 20
Y
 
Layer thickness: 200 mm, particle size 2 to 25 mm, pore size 60Å
Silica gel
aluminum
15 x 10
Y
Silica gel
aluminum
10 x 20
Y
Silica gel
aluminum
20 x 20
Y
 
Layer thickness: 250 mm, particle size 5 to 17 mm, pore size 60Å
Silica gel
glass
15 x 20
Y
Silica gel
glass
15 x 20
Y
Silica gel
glass
10 x 20
-
Silica gel
glass
10 x 20
Y
Silica gel
glass
20 x 20
-
Silica gel
glass
20 x 20
Y
Silica gel
polyester
20 x 20
-
Silica gel
polyester
20 x 20
Y
Silica, highly purified
glass
20 x 20
-
Silica, highly purified
glass
20 x 20
Y
Silica, highly purified
polyester
20 x 20
-
Silica, highly purified
polyester
20 x 20
Y
 
Layer thickness: 250 mm, particle size 2 to 10 mm, pore size 60Å
Chiral-silica gel
glass
15 x 20
-
Chiral-silica gel
glass
10 x 20
-
Chiral-silica gel
glass
20 x 20
-

*PEI plates may turn slightly yellow, affecting separation. Predevelop plates in distilled water to minimize yellow color.


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ACCESSORIES


TLC development chambers

Uses only 2mL of solvent with elution complete in about 45s (using 20% EtOAc/hexanes as eluant). Greater resolution may be achieved by extending elution time. Borosilicate glass. Use with 2.5 x 7cm flexible plastic or glass TLC plates that may be spotted with up to 10 fractions 7mm apart along the edge of the plate.




Performance features:
  • Rapid analysis
  • Highly efficient use of TLC plate
  • Very little solvent required
  • Unlike beakers and jars, tube wall provides a straight base for even solvent flow
No. slots
Dim. L x o.d. (mm)
Catalog Number
Single
95.9 x 25.4
Dual
...190 x 25.4 .
TLC Plates, silica gel; 2.5 x 7.5cm glass plates, 250 mm layer. Package of 100

1) Levine, S.G., J. Chem. Educ.,1996,73,77.

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TLC developing tanks

Can be used as a staining chamber. Made of glass with lid.

O.d. x H (cm)
Catalog Number
6.5 x 10.5
6.5 x 21.0
Replacement tank lid

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TLC developing tanks

Glass with lid.
Inside dim. (cm)
Complete
Catalog Number
Lid Only
Catalog Number
27.5 x 27.5 x 7.5
17.5 x 16.0 x 6.2
17.5 x 16.0 x 8.2
17.5 x 11.0 x 6.2
17.5 x ..6.2 x 6.8
12.1 x 10.8 x 8.3
7.5 x 15.5 x 8.0

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TLC spotting guide and Rf reader

Markings at the bottom of the template are useful guides for applying samples at uniform distances across the plate. The guide is printed on 5mm clear acetate and measures 7 x 8 1/2in. (180 x 215mm).

S 2009


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ALDRICH CHROMATOGRAPHY SPRAYERS

Provide a fine, uniform spray that is optimized for the development of TLC plates. Also suited for use in electrophoresis.
  • Adjustable spray pattern using thumb on vent hole
  • Greaseless, screw-threaded ST joint will not seize; a simple turn of the threaded cap pulls joint apart safely
  • Threaded hose barb prevents accidental breakage during tubing installation or removal
  • Uses low-pressure gas or air (<5psi).
Flask size (mL)
Catalog Number
10
50
125
250


Compressed-air sprayers for spraying TLC plates. Apparatus includes a glass-sprayer head and the designated tube, bottle, or flask.

Bottle-type sprayer
240mL bottle with sprayer head. Z12,630-6
Replacement sprayer head. Z40,726-7

Flask-type sprayers

Erlenmeyer flask with #15 Ace-Thred with sprayer head.

Cap. (mL)
Catalog Number
175
250
Replacement parts  
Erlenmeyer flask with #15 Ace-Thred.
175
250
Sprayer head


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Kimble micro capillary pipettes

Disposable pipettes are calibrated "to contain". Pipettes are 5 inches long and the different sizes are color-coded. Made of soda lime glass. Each cylinder pack comes with one pipette device. Filling is easy. Capillary action draws the fluid into the tube. To dispense, simply cover the hole on the rubber bulb and squeeze.


Cap. (mL)
Tol. (±)
Catalog Number
5
1.0
10
0.5
20
0.5
25
0.5
50
0.5
100
0.5

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