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Antibody Explorer

Affinity Chromatography

Sigma® has prepared product lists for commonly used protocols from Cold Spring Harbor Protocols.

View the entire protocol on BioSupplyNet.com.

Selected Protocol from "Molecular Cloning: A Laboratory Manual," Third Edition, Joseph Sambrook, David W. Russell.

Removal of Cross-reactive Antibodies from Antiserum: Affinity Chromatography

Joseph Sambrook – Peter Maccallum Cancer Institute and The University of Melbourne, Australia
David W. Russell – University of Texas Southwestern Medical Center, Dallas

ABSTRACT
This protocol describes a method for removing antibodies that react with bacterially encoded proteins by passing a crude preparation of immunoglobulins through a column containing immobilized bacterial proteins.


Products Available for this Protocol

Protocol Material Product No. Description Add to Cart
Sodium borate (pH 8.0) B9876 Sodium tetraborate decahydrate, ReagentPlus®, 99.5–105.0%
NaCl S3014 Sodium chloride, for molecular biology, ≥98% (titration)
Pancreatic DNase I D4527 Deoxyribonuclease I from bovine pancreas, Type II, lyophilized powder, Protein ~90 %, ≥2,000 units/mg protein
Tris-Cl (pH 7.5) T5941 Trizma® hydrochloride, for molecular biology, Biotechnology Performance Certified, cell culture tested, ≥99.0% (titration)
MgCl2 M4880 Magnesium chloride, insect cell culture tested
NaOH S8045 Sodium hydroxide, SigmaUltra, ≥98%, pellets (anhydrous)
TBS T5912 Tris Buffered Saline, 10X, solution
Sodium azide S8032 Sodium azide, SigmaUltra
Triton X-100 234729 Triton® X-100

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Product Association Disclaimer: The Sigma-Aldrich products listed for this specific protocol were selected either to match or to supplement the products listed within the actual protocol. The products/reagents from Sigma-Aldrich have been qualified for usage, but may not have been validated for this specific application. Please refer to the detailed product description on the usage of specific products of interest.