Detection

Abberior® Dyes

For Superior Super-Resolution Microscopy Application

Benefits

  • Optimized for brightness and very low background
  • Optimized switching behavior being the key for super-resolution
  • All markers are tested for different super-resolution methods
    • Abberior STAR for STED, confocal and epifluorescence imaging
    • Abberior CAGE & FLIP for PALM, STORM and GSDIM
  • Abberior dyes are recommended by renowned microscope vendors
  • Proprietary, IP protected products
  • Detailed characteristics of the dyes provided, e.g. optimal STED wavelength


Fig1: 3 color STED image of primary hippocampal neurons imaged with the Abberior Instruments Expert Line STED microscope. Please note the characteristic ~190 nm beta II spectrin periodicity along distal axons (green) which is only visible in the STED image. Labelled structures and dyes: beta II Spectrin (green, Abberior STAR580), Bassoon (red, Abberior STAR635P), Actin cytoskeleton (blue, Phalloidin, Oregon Green 488). Sample was prepared by Elisa D’Este @ MPIBPC, Göttingen.












Microscopic methods in life sciences are of tremendous importance for visualization cellular and tissue structures. In recent years, development has reached a revolution in order to overcome the resolution barrier given by the diffraction limit was broken by new microscopy concepts. That enable resolution limit down to about 10 nm and the visualization of cellular structures and molecular interactions reveal new understanding in biological processes. Due to tremendous efforts in the development of super-resolved fluorescence microscopy, The Nobel Prize in Chemistry for 2014 was awarded to Eric Betzig, Stefan W. Hell, and to William E. Moerner.

These super-resolution microscopy principles are based on several technological approaches. Conventional light microscopy enables a resolution limit of about 250 nm in the x- and y- direction and 450 – 700 nm in the z –direction. Super-resolution techniques are overcome the resolution-limit (Point-spread function), at least by a factor of 2. The resolution of super-resolution microscopy depends on the number of points that can be resolved on the structure of interest. Crucial for a successful super-resolution imaging is the choice of fluorescent probe. Brightness and high contrast ratio between the states are of great importance. In most super-resolution methods, the states of the probe must be controllable, reversible or irreversible, switchable between a light or a dark state. Depending on the super-resolution method, further photo-physical criteria the probe must be fulfilled. Established techniques are, for example:

  • STED (Stimulated emission depletion)
  • GSDIM (Ground State Depletion)
  • PALM (Photoactivated localization microscopy)
  • STORM (Stochastic optical reconstruction microscopy)
  • RESOLFT (reversible saturable optical (flurorescence) transitions)

Sigma now offer the superior series of Abberior dyes, that are especially designed and tested for super-resolution microscopy such as STED, RESOLFT, PALM, STORM, GSDIM and others. Abberior STAR, Abberior CAGE, Abberior FLIP, Abberior RSFP – the specific requirements of the super-resolution techniques are served with dedicated dye series. These Dyes are developed and produced by Abberior GmbH. Stefan Hell is it’s Co-founder.

Super-resolution microscopy depends on fluorescent labels more than any other fluorescence imaging technique. Manufactured by Abberior, the STAR, CAGE and FLIP dyes as well as RSFPs are exceptionally bright and photostable and provide optimized photoswitching for RESOLFT and PALM/STORM imaging. They are the only commercially available dyes that are tailored specifically to the needs of super-resolution microscopy.

Abberior dyes are also exceptionally well suited for confocal microscopy, epifluorescence imaging and single molecule applications. Basically all fluorescence applications which depend on a good signal to noise ratio and low background benefit from the novel Abberior dyes.

Fig2: Two subunits of the nuclear pore complex were immunolabelled using antibodies against gp210 and antibodies with multiple specificities (PAN4/5) and secondary antibodies coupled to Abberior STAR580 and Abberior STAR635P. Note that gp210 is localized in an eightfold symmetric structure at the rim of the nuclear pore complex. Imaged with the Abberior Instruments STEDYCON (compact line).

For further information, please contact monika.baeumle@sial.com


Product Table: Overview of Abberior Dyes

Dyes Description Absorption Maximum/ λmax Extinction Coefficient, ε(λ) Fluorescence Maximum, λfl Recommended STED Cat No., NHS activated Cat No., maleimid activated
Abberior CAGE 500 for single-molecule switching microscopy (e.g. PALM, STORM, GSDIM) 300 nm (caged,pH 7); 501 nm (uncaged, pH 7) 85,000 – 88,000 M-1cm-1 (pH 7, uncaged) 524 nm (pH 7), 523 nm (MeOH) 595-615 nm 44254 92546
Abberior CAGE 532 for single-molecule switching microscopy (e.g. PALM, STORM, GSDIM) 304 nm (caged, PBS, pH 7); 518 nm (uncaged, pH 7) 29,000 M-1cm-1 (pH 7, uncaged) 541 nm (pH 7) 610 - 640 nm 38977 95705
Abberior CAGE 552 for single-molecule switching microscopy (e.g. PALM, STORM, GSDIM) 300 nm (caged,pH 7); 552 nm (uncaged, pH 7) 66,000 M-1cm-1 (pH 7, uncaged) 574 nm (pH 7) 650-670 nm 94822 92545
Abberior CAGE 590 for single-molecule switching microscopy (e.g. PALM, STORM, GSDIM) caged: 295 nm (pH 7); uncages 586 nm (pH 7) 43,800 M-1cm-1 (pH 7, uncaged) 607 nm (pH 7) 685 — 715 nm 77958 no
Abberior FLIP 565 for single-molecule switching microscopy (e.g. PALM, STORM, GSDIM) 314 nm (PBS, pH 7.4) 51,000 M-1cm-1 (MeOH) 580 nm (PBS, pH 7.4)   79189 92544
Abberior STAR 440SX for long Stokes STED and 2-color STED application 430 nm (MeOH), 437 nm (PBS, pH 7.4) 30,800 M-1cm-1 (MeOH), 22,700 M-1cm-1 (PBS, pH 7.4) 501 nm (MeOH), 515 nm (PBS, pH 7.4) 590-620 nm 68221 38361
Abberior STAR 470SX for long Stokes STED and 2-color STED application 475 nm (MeOH), 477 nm (PBS, pH 7.4) 30,400 M-1cm-1 (MeOH), 22,700 M-1cm-1 (PBS, pH 7.4) 609 nm (MeOH), 627 nm (PBS, pH 7.4) 740 - 770 nm 95348 no
Abberior STAR 488 for STED application 501 nm (PBS, pH 7.4) 86,000 M-1cm-1 (MeOH) 524 nm (PBS, pH 7.4) 585 - 605 nm 61048 no
Abberior STAR 512 for STED application 517 nm (MeOH), 512 nm (PBS, pH 7.4) 74,000 M-1cm-1 (MeOH) 536 nm (MeOH), 530 nm (PBS, pH 7.4) 590 - 620 nm 38922 03004
Abberior STAR 580 for STED application 587 nm (MeOH), 583 nm (PBS, pH 7.4) 64,300 M-1cm-1 (MeOH) 609 nm (MeOH), 605 nm (PBS, pH 7.4) 690 - 720 nm 38377 no
Abberior STAR 635 for STED application 639 nm (MeOH), 634 nm (PBS, pH 7.4) 63,000 M-1cm-1 (MeOH) 659 nm (MeOH), 654 nm (PBS, pH 7.4) 740 - 770 nm 30558 96013
Abberior STAR 635P for STED application 635 nm (MeOH), 634 nm (PBS, pH 7.4) 80,000 M-1cm-1 (water) 655 nm (MeOH), 654 nm (PBS, pH 7.4) 740 - 770 nm 07679 no, only availabe as Azide derivative, Cat. No. 95408

 

For North Amerika, we also provide a series of antibody labeled Abberior conjugates.
 

Cat No. Description Availability Pack size
53647 Anti-Rabbit IgG-Abberior® CAGE 635 antibody produced in goat US only 500ug
30483 Anti-Mouse IgG-Abberior® CAGE 635 antibody produced in goat US only 500ug
54287 Anti-Rabbit IgG-Abberior® CAGE 590 antibody produced in goat US only 500ug
53364 Anti-Mouse IgG-Abberior® CAGE 590 antibody produced in goat US only 500ug
40544 Anti-Rabbit IgG-Abberior® CAGE 552 antibody produced in goat US only 500ug
53165 Anti-Mouse IgG-Abberior® CAGE 552 antibody produced in goat US only 500ug
53601 Anti-Rabbit IgG-Abberior® CAGE 532 antibody produced in goat US only 500ug
52996 Anti-Mouse IgG-Abberior® CAGE 532 antibody produced in goat US only 500ug
41155 Anti-Rabbit IgG-Abberior® CAGE 500 antibody produced in goat US only 500ug
52953 Anti-Mouse IgG-Abberior® CAGE 500 antibody produced in goat US only 500ug
52283 Anti-Mouse IgG-Abberior® STAR  RED antibody produced in goat US only 500ug
53399 Anti-Rabbit IgG-Abberior® STAR 635P antibody produced in goat US only 500ug
41699 Anti-Rabbit IgG-Abberior® STAR RED antibody produced in goat US only 500ug
41348 Anti-Rabbit IgG-Abberior® STAR 635 antibody produced in goat US only
(available soon)
500ug
40734 Anti-Mouse IgG-Abberior® STAR 635 antibody produced in goat US only 500ug
53654 Anti-Rabbit IgG-Abberior® STAR 600 antibody produced in goat US only 500ug
41367 Anti-Rabbit IgG-Abberior® STAR 580 antibody produced in goat US only 500ug
52597 Anti-Mouse IgG-Abberior® STAR 600 antibody produced in goat US only 500ug
52403 Anti-Mouse IgG-Abberior® STAR 580 antibody produced in goat US only 500ug
52932 Anti-Rabbit IgG-Abberior® STAR 520SXP antibody produced in goat US only 500ug
41372 Anti-Mouse IgG-Abberior® STAR  520SXP antibody produced in goat US only 500ug
52269 Anti-Mouse IgG-Abberior® STAR 512 antibody produced in goat US only 500ug
00289 Anti-Rabbit IgG-Abberior® STAR 512 antibody produced in goat US only 500ug
52944 Anti-Rabbit IgG-Abberior® STAR 488 antibody produced in goat US only 500ug
53366 Anti-Mouse IgG-Abberior® STAR 488 antibody produced in goat US only 500ug
52187 Anti-Mouse IgG-Abberior® STAR 470SXP antibody produced in goat US only 500ug
41324 Anti-Rabbit IgG-Abberior® STAR 470SXP antibody produced in goat US only 500ug
41860 Anti-Rabbit IgG-Abberior® STAR 440SXP antibody produced in goat US only 500ug
41738 Anti-Mouse IgG-Abberior® STAR 440SXP antibody produced in goat US only 500ug

 

 

  1. Leica Microsystems recommendations for 2-color applications.
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  8. Arnaud P. Giese, Jérome Ezan, Lingyan Wang, Léa Lasvaux, Frédérique Lembo, Claire Mazzocco, Elodie Richard, Jérome Reboul, Jean-Paul Borg, Matthew W. Kelley, Nathalie Sans, John Brigande, Mireille Montcouquiol "Gipc1 has a dual role in Vangl2 trafficking and hair bundle integrity in the inner ear", Development 139, pp. 3775-3785, DOI:10.1242/dev.074229 (2012)
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  10. S. Li, C.-f. Kuang, Y.-f. Wang, X. Hao, P. Xiu, Y.-k. Xu, X. Liu, "High Speed Optical Nanoscopy by Stimulated Emission Depletion (STED) with Galvo Mirrors", roc. SPIE 8911, International Symposium on Photoelectronic Detection and Imaging 2013: Micro/Nano Optical Imaging Technologies and Applications (2013)
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  15. K. Kolmakov, C. A. Wurm, R. Hennig, E. Rapp, S. Jakobs, V. N. Belov, S. W. Hell, "Red-Emitting Rhodamines with Hydroxylated, Sulfonated, and Phosphorylated Dye Residues and Their Use in Fluorescence Nanoscopy" Chem. Eur. J. 2012, 18, 12986 –12998 (2013)
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  19. I.-H. Wang, M- Suomalainen, V. Andriasyan, S. Kilcher, J. Mercer, A. Neef, N. W. Luedtke, U. F. Greber, "Tracking Viral Genomes in Host Cells and Single Molecule Resolution", Cell Host Microbe, 14(4), pp. 468–480, DOI: 10.1016/j.chom.2013.09.004 (2013)
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  22. M. V. Sednev, C. A. Wurm, V. N. Belov, S. W. Hell, "Carborhodol: A New Hybrid Fluorophore Obtained by Combination of Fluorescein and Carbopyronine Dye Cores", Bioconjugate Chem., 24(4), pp 690–700, DOI: 10.1021/bc3006732 (2013)
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