Abberior^® Dyes

For Superior Super-Resolution Microscopy Application

Benefits

• Optimized for brightness and very low background
• Optimized switching behavior being the key for super-resolution
• All markers are tested for different super-resolution methods
  • Abberior STAR for STED, confocal and epifluorescence imaging
  • Abberior CAGE & FLIP for PALM, STORM and GSDIM
• Abberior dyes are recommended by renowned microscope vendors
• Proprietary, IP protected products
• Detailed characteristics of the dyes provided, e.g. optimal STED wavelength

Microscopic methods in life sciences are of tremendous importance for visualization cellular and tissue structures. In recent years, development has reached a revolution in order to overcome the resolution barrier given by the diffraction limit was broken by new microscopy concepts. That enable resolution limit down to about 10 nm and the visualization of cellular structures and molecular interactions reveal new understanding in biological processes. Due to tremendous efforts in the development of super-resolved fluorescence microscopy, The Nobel Prize in Chemistry for 2014 was awarded to Eric Betzig, Stefan W. Hell, and to William E. Moerner.

These super-resolution microscopy principles are based on several technological approaches. Conventional light microscopy enables a resolution limit of about 250 nm in the x- and y- direction and 450 – 700 nm in the z –direction. Super-resolution techniques are overcome the resolution-limit (Point-spread function), at least by a factor of 2. The resolution of super-resolution microscopy depends on the number of points that can be resolved on the structure of interest. Crucial for a successful super-resolution imaging is the choice of fluorescent probe. Brightness and high contrast ratio between the states are of great importance. In most super-resolution methods, the states of the probe must be controllable, reversible or irreversible, switchable between a light or a dark state. Depending on the super-resolution method, further photo-physical criteria the probe must be fulfilled. Established techniques are, for example:

• STED (Stimulated emission depletion)
• GSDIM (Ground State Depletion)
• PALM (Photoactivated localization microscopy)
• STORM (Stochastic optical reconstruction microscopy)
• RESOLFT (reversible saturable optical (flurorescence) transitions)

Sigma now offer the superior series of Abberior dyes, that are especially designed and tested for super-resolution microscopy such as STED, RESOLFT, PALM, STORM, GSDIM and others. Abberior STAR, Abberior CAGE, Abberior FLIP, Abberior RSFP – the specific requirements of the super-resolution techniques are served with dedicated dye series. These Dyes are developed and produced by Abberior GmbH. Stefan Hell is it’s Co-founder.

Super-resolution microscopy depends on fluorescent labels more than any other fluorescence imaging technique. Manufactured by Abberior, the STAR, CAGE and FLIP dyes as well as RSFPs are exceptionally bright and photostable and provide optimized photoswitching for RESOLFT and PALM/STORM imaging. They are the only commercially available dyes that are tailored specifically to the needs of super-resolution microscopy.

Abberior dyes are also exceptionally well suited for confocal microscopy, epifluorescence imaging and single molecule applications. Basically all fluorescence applications which depend on a good signal to noise ratio and low background benefit from the novel Abberior dyes.

For further information, please contact monika.baeumle@sial.com


Product Table: Overview of Abberior Dyes

For North Amerika, we also provide a series of antibody labeled Abberior conjugates.
 

1. Leica Microsystems recommendations for 2-color applications.
2. T. Müller, C. Schumann, A. Kraegeloh, STED Microscopy and its Applications: New Insights into Cellular Processes on the Nanoscale, ChemPhysChem 13, 1986–2000 (2012).
3. Tim Grotjohann, Ilaria Testa, Marcel Leutenegger, Hannes Bock, Nicolai T. Urban, Flavie Lavoie-Cardinal, Katrin I. Willig, Christian Eggeling, Stefan Jakobs, Stefan W. Hell, Diffraction-unlimited all-optical imaging and writing with a photochromic GFP, Nature 478, 204-208 (13 October 2011).
4. V. N. Belov et al., "Rhodamines NN: A Novel Class of Caged Fluorescent Dyes", Angew. Chem. Int. Ed. 49, 3520−3523 (2010).
5. T. Karlsson, M. V. Turkina, O. Yakymenko, K.-E. Magnusson, E. Vikström "The Pseudomonas aeruginosa N-Acylhomoserine Lactone Quorum Sensing Molecules Target IQGAP1 and Modulate Epithelial Cell Migration", PLoS Pathog, 8(10), e1002953, DOI: 10.1371/journal.pp (2012).
6. Xinxin Zhu , Ya-Ting Kao , Wei Min "Molecular-Switch-Mediated Multiphoton Fluorescence Microscopy with High-Order Nonlinearity", J. Phys. Chem. Lett., 3 (15), pp. 2082–2086, DOI: 10.1021/jz300607c (2012).
7. Francisco Balzarotti , Fernando D. Stefani "Plasmonics Meets Far-Field Optical Nanoscopy", ACS Nano, 6 (6), pp. 4580–4584, DOI: 10.1021/nn302306m (2012).
8. Arnaud P. Giese, Jérome Ezan, Lingyan Wang, Léa Lasvaux, Frédérique Lembo, Claire Mazzocco, Elodie Richard, Jérome Reboul, Jean-Paul Borg, Matthew W. Kelley, Nathalie Sans, John Brigande, Mireille Montcouquiol "Gipc1 has a dual role in Vangl2 trafficking and hair bundle integrity in the inner ear", Development 139, pp. 3775-3785, DOI:10.1242/dev.074229 (2012)
9. Nicolas Olivier, Debora Keller, Vinoth Sundar Rajan, Pierre Gönczy, Suliana Manley "Simple buffers for 3D STORM microscopy", Biomedical Optics Express, 4 (6), pp. 885-899, DOI: 10.1364/BOE.4.000885 (2013)
10. S. Li, C.-f. Kuang, Y.-f. Wang, X. Hao, P. Xiu, Y.-k. Xu, X. Liu, "High Speed Optical Nanoscopy by Stimulated Emission Depletion (STED) with Galvo Mirrors", roc. SPIE 8911, International Symposium on Photoelectronic Detection and Imaging 2013: Micro/Nano Optical Imaging Technologies and Applications (2013)
11. F. C. Zanacchi, Z. Lavagnino, M. Faretta, L. Furia, A. Diaspro, "Light-Sheet Confined Super-Resolution Using Two-Photon Photoactivation", PloS ONE, 8(7): e67667 (2013)
12. G. Vicidomini, I. C. Hernández, M. D'Amora, F. C. Zanacchi, P. Bianchini, A. Diaspro, "Gated CW-STED Microscopy: A Versatile Tool for Biological Nanometer Scale Investigation", Methods, science direct (2013)
13. H. Blom, D. Rönnlund, L. Scott, L. Westin, J. Widengren, A. Aperia, H. Brismar, „Spatial Distribution of DARPP-32 in Dendritic Spines“, PloS ONE, 8(9) (2013)
14. F. Göttfert, C.A. Wurm, V. Müller, S. Berning, V.C. Cordes, A. Honigmann, S.W. Hell, „Coaligned Dual-Channel STED Nanoscopy and molecular diffusion analysis at 20 nm resolution“, Biophysical Journal, Volume 105, Issue 1(2013)
15. K. Kolmakov, C. A. Wurm, R. Hennig, E. Rapp, S. Jakobs, V. N. Belov, S. W. Hell, "Red-Emitting Rhodamines with Hydroxylated, Sulfonated, and Phosphorylated Dye Residues and Their Use in Fluorescence Nanoscopy" Chem. Eur. J. 2012, 18, 12986 –12998 (2013)
16. C. A. Wurm, K. Kolmakov, F. Göttfert, H. Ta, M. L. Bossi, H. Schill, S. Berning, S. Jakobs, G. Donnert, V. N. Belov, S. W. Hell, "Novel red fluorophores with superior performance in STED microscopy", Optical Nanoscopy 2012; DOI: 10.1186/ 2192-2853-1-7 (2013)
17. S. Rocha, H. De Keersmaecker, H. Uji-i, J. Hofkens, H. Mizuno, "Photoswitchable Fluorescent Proteins for Superresolution Fluorescence Microscopy Circumventing the Diffraction Limit of Light" in Fluorescence Spectroscopy and Microscopy (Eds.: Y. Engelborghs, A. J. W. G. Visser), Humana Press, Vol. pp. 793-812 (2013)
18. J. V. Chacko, F. C. Zanacchi, A. Diaspro, "Probing Cytoskeletal Structures by Coupling Optical Superresolution and AFM Techniques for a Correlative Approach", Cytoskeleton, 70(11), pp. 729–740, DOI: 10.1002/cm.21139 (2013)
19. I.-H. Wang, M- Suomalainen, V. Andriasyan, S. Kilcher, J. Mercer, A. Neef, N. W. Luedtke, U. F. Greber, "Tracking Viral Genomes in Host Cells and Single Molecule Resolution", Cell Host Microbe, 14(4), pp. 468–480, DOI: 10.1016/j.chom.2013.09.004 (2013)
20. H. Schill, S. Nizamov, F. Bottanelli, J. Bierwagen, V. N. Belov, S. W. Hell, "4-Trifluoromethyl-Substituted Coumarins with Large Stokes Shifts: Synthesis, Bioconjugates, and Their Use in Super-Resolution Fluorescence Microscopy", Chem. Eur. J., 19(49), pp. 16556–16565, DOI: 10.1002/chem.201302037 (2013)
21. Y. Wang, C. Kuang, Z. Gu, Y. Xu, S. Li, X. Hao, X. Liu, "Time-Gated Stimulated Emission Depletion Nanoscopy", Opt. Eng., 52(9), 093107, DOI: 10.1117/1.OE.52.9.093107 (2013)
22. M. V. Sednev, C. A. Wurm, V. N. Belov, S. W. Hell, "Carborhodol: A New Hybrid Fluorophore Obtained by Combination of Fluorescein and Carbopyronine Dye Cores", Bioconjugate Chem., 24(4), pp 690–700, DOI: 10.1021/bc3006732 (2013)
23. The Nobel Prize in Chemistry 2014". Nobelprize.org. Nobel Media AB 2014. Web. 14 Oct 2014.

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