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Detection

Tracability

The kit has been developed according to ISO Guides 34 and 35 and the standards are traceable to the spectral radiance scale. The wavelength-dependent relative uncertainties of the corrected emission spectra combine the relative uncertainties of the calibration of the fluorometer used for certification and the measurements of the emission spectra as well as material-related uncertainties derived from homogeneity and stability studies according to ISO Guide 35.

Both the corrected emission spectra and the determination of a kit-based emission correction curve with the Kit and LINKCORR have been evaluated in an international Round Robin of the National Metrological Institutes NIST, NRC (National Research Council, Canada), NPL (National Physics Laboratory, UK), and PTB with four traceably characterized instruments and two different measurement geometries, i.e., 0°/90° and 45°/0°. Moreover, this calibration tool has been successfully tested with several types of common spectrofluorometers by selected users from industry and academics. Results that are exemplary depicted in Fig. 4 that compares the uncorrected and Kit-corrected emission spectra of three tests dyes X, QS, and Y reveal a comparability of Kit-based corrected fluorescence spectra better than 10 percent. This is similar to what is achievable with a much more tedious and time consuming procedure and severe geometry restrictions if physical transfer standards are used in an expert laboratory






Proficiency testing of the Kit Spectral Fluorescence Standards. Kit-based spectral correction of the uncorrected emission spectra of the test dyes X, QS, and Y (top) measured with four fluorometers and comparison of the resulting corrected emission spectra (bottom). A comparability better than 10% is achieved.


Certified Fluorescence Standard
Instrument Effects on Fluorescence Signals
Determination of the Spectral Responsivity
Kit Spectral Fluorescence Standards: Principle and Properties
References



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