Arrays & Interaction Profiling

Panorama® Antibody Arrays

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Panorama Antibody Array Technology
Protein expression profiling... broad and fast

  • Rapid, multiplex analysis of protein expression
  • Antibodies react with human, mouse, and rat proteins
  • Compatible with Cy3/Cy5 detection
  • Use serum, cell or tissue samples

The development of antibody-based technologies has been driven by the often-poor correlation between mRNA and protein expression. Sigma’s Panorama Antibody Array technology enables researchers to screen expression changes in large numbers of proteins in significantly less time than it would take using conventional protein detection methods due to the antibody detection sensitivity.

Panorama Antibody Microarray kits are designed for studying protein expression in cell or tissue extracts. The Panorama Antibody Microarray kits encompass the areas of cell signaling proteins, kinases and phosphatases of the MAPK and PKC pathways, chromatin remodeling and gene expression, including transcription factors, histone modifying enzymes, and various P53 pathways. With market-leading content, the Panorama Antibody Array - XPRESS Profiler725 provides a high-throughput tool for multiplex expression profiling of the most important proteome-related activities.

Gene Regulation | MAPK & PKC Pathways
Frequently Asked Questions | Data | Literature | Webinar | Ordering Information

Gene Regulation (Product No. GRAA2)
Kit contains 112 different antibodies, representing families of proteins found in the nucleus including transcription factors, histone modifying enzymes, and antibodies that specifically recognize histone modification.

MAPK & PKC Pathways (Product No. MPAA3)
Kit contains 84 different antibodies specific for kinases and phosphatases of the MAPK and PKC pathways.

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Data

Protein Expression in F9 Mouse Stem Cells Induced by Retinoic Acid

Protein Expression in F9 Mouse Stem Cells Induced by Retinoic Acid Image

Figure 1. Expression of proteins in F9 mouse stem cells induced to differentiate by retinoic acid utilizing the Panorama Antibody Array-Cell Signaling. The total cell extract of F9 cells non-treated or treated for four days with retinoic acid were prepared and labeled with Cy3 and Cy5, respectively. Equal amounts of Cy3 or Cy5 labeled protein were incubated on the slide. The experiment was repeated at least three times. Proteins marked in red were up-regulated while those in green were down-regulated.

 

Differential Expression between Different Tissue Types

Differential Expression between Different Tissue Types

Figure 2. Differential protein expression in mouse tissues utilizing the Panorama Antibody Array-Cell Signaling. Equal concentration of mouse tissue extract (5 µg/mL) from liver, lung, and cerebellum were incubated on different slides.


Ordering Information

Product No. Description Add to Cart
GRAA2 Panorama Antibody Microarray - Gene Regulation Kit
MPAA3 Panorama Antibody Microarray - MAPK & PKC Pathways Kit

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