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Cell Culture

Basal Media Formulation

Component B1522
[1x] g/L
B9638
g/L
Inorganic Salts
Calcium Chloride 0.2 0.2
Magnesium Sulfate (anhydrous) 0.09767 0.09767
Potassium Chloride 0.4 0.4
Sodium Bicarbonate 2.2
Sodium Chloride 6.8 6.8
Sodium Phosphate Monobasic (anhydrous) 0.122 0.122
Amino Acids
L-Arginine • HCl 0.021 0.021
L-Cystine • 2HCl 0.01565 0.01565
L-Glutamine 0.292
L-Histidine (free base) 0.008 0.008
L-Isoleucine 0.026 0.026
L-Leucine 0.026 0.026
L-Lysine • HCl 0.03647 0.03647
L-Methionine 0.0075 0.0075
L-Phenylalanine 0.0165 0.0165
L-Threonine 0.024 0.024
L-Tryptophan 0.004 0.004
L-Tyrosine • 2Na • 2H2O 0.02595 0.02595
L-Valine 0.0235 0.0235
Vitamins
D-Biotin 0.001 0.001
Choline Chloride 0.001 0.001
Folic Acid 0.001 0.001
myo-Inositol 0.002 0.002
Niacinamide 0.001 0.001
D-Pantothenic Acid (hemicalcium) 0.001 0.001
Pyridoxal • HCl 0.001 0.001
Riboflavin 0.0001 0.0001
Thiamine • HCl 0.001 0.001
Other
D-Glucose 1 1
Phenol Red • Na 0.011 0.011
Add
L-Glutamine 0.292
Sodium Bicarbonate 2.2
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