Attention:

Certain features of Sigma-Aldrich.com will be down for maintenance the evening of Friday August 18th starting at 8:00 pm CDT until Saturday August 19th at 12:01 pm CDT.   Please note that you still have telephone and email access to our local offices. We apologize for any inconvenience.

Cell Culture

Dulbecco's Modified Eagle's Medium/Ham's Nutrient Mixture F-12 (DME/F12) Formulation

Click on the product numbers at the top of each column.

Component D0547
g/L
D2906
g/L
D6421
[1x] g/L
D6434
[1x] g/L
D8062
[1x] g/L
D8437
[1x] g/L
D8900
g/L
D9785
g/L
Inorganic Salts
Ammonium Molybdate • 4H2O 0.00000618
Ammonium Metavandate 0.00000058
Calcium Chloride 0.1166 0.1545 0.1166 0.1545 0.1166 0.1545 0.1545
Cupric Sulfate • 5H2O 0.0000013 0.0000013 0.0000013 0.0000013 0.0000013 0.0000013 0.0000013 0.0000013
Ferric Nitrate • 9H2O 0.00005 0.00005 0.00005 0.00005 0.00005 0.00005 0.00005 0.00005
Ferrous Sulfate • 7H2O 0.000417 0.000417 0.000417 0.000417 0.000417 0.000417 0.000417 0.000417
Manganese Sulfate 0.000000151
Magnesium Chloride • 6H2O 0.0612 0.0612 0.0612 0.0612 0.0612 0.0612 0.0612
Magnesium Sulfate (anhydrous) 0.04884 0.04884 0.04884 0.04884 0.04884 0.04884 0.04884
Nickel Chloride 0.00000012
Potassium Chloride 0.3118 0.3118 0.3118 0.3118 0.3118 0.3118 0.3118 0.3118
Sodium Bicarbonate 1.2 1.2
Sodium Chloride 6.996 6.996 6.996 6.996 6.996 6.996 6.996 6.996
Sodium Metasilicate • 9H2O 0.0000142
Sodium Selenite 0.00000519
Sodium Phosphate Dibasic (anhydrous) 0.07102 0.07102 0.07102 0.07102 0.07102 0.07102 0.07102 0.07102
Sodium Phosphate Monobasic (anhydrous) 0.0543 0.0543 0.0543 0.0543 0.0543 0.0543 0.0543 0.0543
Stannous Chloride • 2H2O 0.00000011
Zinc Sulfate • 7H2O 0.000432 0.000432 0.000432 0.000432 0.000432 0.000432 0.000432 0.000432
Amino Acids
L-Alanine 0.00445 0.00445 0.00445 0.00445 0.00445 0.00445 0.00445 0.00445
L-Arginine • HCl 0.1475 0.1475 0.1475 0.1475 0.1475 0.1475 0.1475 0.1475
L-Asparagine • H2O 0.0075 0.0075 0.0075 0.0075 0.0075 0.0075 0.0075 0.0075
L-Aspartic Acid 0.00665 0.00665 0.00665 0.00665 0.00665 0.00665 0.00665 0.00665
L-Cystine • 2HCl 0.03129 0.03129 0.03129 0.03129 0.03129 0.03129 0.03129 0.03129
L-Cysteine • HCl • H2O 0.01756 0.01756 0.01756 0.01756 0.01756 0.01756 0.01756 0.01756
L-Glutamic Acid 0.00735 0.00735 0.00735 0.00735 0.00735 0.00735 0.00735 0.00735
L-Glutamine 0.365 0.365 0.365 0.365 0.365
Glycine 0.01875 0.01875 0.01875 0.01875 0.01875 0.01875 0.01875 0.01875
L-Histidine • HCl • H2O 0.03148 0.03148 0.03148 0.03148 0.03148 0.03148 0.03148 0.03148
L-Isoleucine 0.5447 0.5447 0.5447 0.05447 0.05447 0.05447 0.05447 0.05447
L-Leucine 0.05905 0.05905 0.05905 0.05905 0.05905 0.05905 0.05905
L-Lysine • HCl 0.09125 0.09125 0.09125 0.09125 0.09125 0.09125 0.09125
L-Methionine 0.01724 0.01724 0.01724 0.01724 0.01724 0.01724 0.01724
L-Phenylalanine 0.03548 0.03548 0.03548 0.03548 0.03548 0.03548 0.03458 0.03548
L-Proline 0.01725 0.01725 0.01725 0.01725 0.01725 0.01725 0.01725 0.01725
L-Serine 0.02625 0.02625 0.02625 0.02625 0.02625 0.02625 0.02625 0.02625
L-Threonine 0.05345 0.05345 0.05345 0.05345 0.05345 0.05345 0.05345 0.05345
L-Tryptophan 0.00902 0.00902 0.00902 0.00902 0.00902 0.00902 0.00902 0.00902
L-Tyrosine • 2Na • 2H2O 0.05579 0.05579 0.05579 0.05579 0.05579 0.05579 0.05579 0.05579
L-Valine 0.05285 0.05285 0.05285 0.05285 0.05285 0.05285 0.05285 0.05285
Vitamins
D-Biotin 0.0000035 0.0000035 0.0000035 0.0000035 0.0000035 0.0000035 0.0000035 0.0000035
Choline Chloride 0.00898 0.00898 0.00898 0.00898 0.00898 0.00898 0.00898 0.00898
Folic Acid 0.00265 0.00265 0.00265 0.00266 0.00266 0.00266 0.00266 0.00266
myo-Inositol 0.0126 0.0126 0.0126 0.0126 0.0126 0.0126 0.0126 0.0126
Niacinamide 0.00202 0.00202 0.00202 0.00202 0.00202 0.00202 0.00202 0.00202
D-Pantothenic Acid (hemicalcium) 0.00224 0.00224 0.00224 0.00224 0.00224 0.00224 0.00224 0.00224
Pyridoxal • HCl 0.002 0.002 0.002 0.002 0.002 0.002 0.002
Pyridoxine • HCl 0.002031 0.002031 0.002031 0.000031 0.000031 0.000031
Riboflavin 0.000219 0.000219 0.000219 0.000219 0.000219 0.000219 0.000219 0.000219
Thiamine • HCl 0.00217 0.00217 0.00217 0.00217 0.00217 0.00217 0.00217 0.00217
Vitamin B12 0.00068 0.00068 0.00068 0.00068 0.00068 0.00068 0.00068 0.00068
Other
D-Glucose 3.15 3.15 3.15 3.15 3.15 3.15 3.15 3.15
HEPES 3.5745 3.5745 3.5745 3.5745 3.5745 3.5745
Hypoxanthine 0.0021 0.00244 0.0021 0.00244 0.0021 0.00244 0.00244 0.0021
Linoleic Acid 0.000042 0.000042 0.000042 0.000042 0.000042 0.000042 0.000042 0.000042
Phenol Red • Na 0.00863 0.00863 0.00863 0.00863 0.00863
Putrescine • HCl 0.000081 0.000081 0.000081 0.000081 0.000081 0.000081 0.000081 0.000081
Pyruvic Acid • Na 0.11 0.055 0.055 0.055 0.055 0.055 0.055 0.055
DL-Thioctic Acid 0.000105 0.000105 0.000105 0.000105 0.000105 0.000105 0.000105 0.000105
Thymidine 0.000365 0.000365 0.000365 0.000365 0.000365 0.000365 0.000365 0.000365
Add
Sodium Bicarbonate 1.2 1.2 1.2 1.2
Calcium Chloride • 2H2O 0.1545
L-Glutamine 0.365 0.365 0.365
L-Leucine 0.05905
L-Lysine • HCl 0.09125
Magnesium Chloride • 6H2O 0.0612
Magnesium Sulfate 0.04884
L-Methionine 0.0172
back to top