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Cell Culture

Glasgow Minimum Essential Medium Formulation

Component G5154
[1x] g/L
G6148
g/L
Inorganic Salts
Calcium Chloride 0.2 0.2
Ferric Nitrate • 9H2O 0.0001 0.0001
Magnesium Sulfate (anhydrous) 0.09767 0.09767
Potassium Chloride 0.4 0.4
Sodium Bicarbonate 2.75
Sodium Chloride 6.4 6.4
Sodium Phosphate Monobasic (anhydrous) 0.1078 0.1078
Amino Acids
L-Arginine • HCl 0.042 0.042
L-Cystine • 2HCl 0.03129 0.03129
L-Glutamine 0.292
L-Histidine • HCl • H2O 0.021 0.021
L-Isoleucine 0.0524 0.0524
L-Leucine 0.0524 0.0524
L-Lysine • HCl 0.0731 0.0731
L-Methionine 0.015 0.015
L-Phenylalanine 0.033 0.033
L-Threonine 0.0476 0.0476
L-Tryptophan 0.008 0.008
L-Tyrosine • 2Na • H2O 0.05219 0.05219
L-Valine 0.0468 0.0468
Vitamins
Choline Chloride 0.002 0.002
Folic Acid 0.002 0.002
myo-Inositol 0.0036 0.0036
Niacinamide 0.002 0.002
D-Pantothenic Acid (hemicalcium) 0.002 0.002
Pyridoxal • HCl 0.002 0.002
Riboflavin 0.0002 0.0002
Thiamine • HCl 0.002 0.002
Other
D-Glucose 4.5 4.5
Phenol Red • Na 0.016 0.016
Add
Sodium Bicarbonate 2.75
L-Glutamine 0.292
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