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Cell Culture

L-15 Media Formulation

Component L1518
[1x] g/L
L4386
[1x] g/L
L5520
g/L
Inorganic Salts
Calcium Chloride 0.1396 0.1396 0.1396
Magnesium Chloride 0.0937 0.0937 0.0937
Magnesium Sulfate (anhydrous) 0.09767 0.09767 0.09767
Potassium Chloride 0.4 0.4 0.4
Potassium Phosphate Monobasic (anhydrous) 0.06 0.06 0.06
Sodium Chloride 8 8 8
Sodium Phosphate Dibasic (anhydrous) 0.19 0.19 0.19
Amino Acids
L-Alanine 0.225 0.225 0.225
L-Arginine (free base) 0.5 0.5 0.5
L-Asparagine (anhydrous) 0.25 0.25 0.25
L-Cysteine (free base) 0.12 0.12 0.12
L-Glutamine 0.3 0.3
Glycine 0.2 0.2 0.2
L-Histidine 0.25 0.25 0.25
L-Isoleucine 0.125 0.125 0.125
L-Leucine 0.125 0.125 0.125
L-Lysine 0.0937 0.0937 0.0937
L-Methionine 0.075 0.075 0.075
L-Phenylalanine 0.125 0.125 0.125
L-Serine 0.2 0.2 0.2
L-Threonine 0.3 0.3 0.3
L-Tryptophan 0.02 0.02 0.02
L-Tyrosine (free base) 0.3 0.3 0.3
L-Valine 0.1 0.1 0.1
Vitamins
Choline Chloride 0.001 0.001 0.001
Flavin Mononucleotide • Na 0.0001 0.0001 0.0001
Folic Acid 0.001 0.001 0.001
myo-Inositol 0.002 0.002 0.002
Niacinamide 0.001 0.001 0.001
DL-Pantothenic Acid (hemicalcium) 0.001 0.001 0.001
Pyridoxine • HCl 0.001 0.001 0.001
Thiamine Monophosphate • HCl 0.001 0.001 0.001
Other
D-Galactose 0.9 0.9 0.9
Phenol Red • Na 0.011 0.011 0.011
Pyruvic Acid • Na 0.55 0.55 0.55
Add
L-Glutamine 0.3
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