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Cell Culture

McCoy's 5A Modified Media Formulation

Component M4892
g/L
M8403
[1x] g/L
Inorganic Salts
Calcium Chloride 0.09995 0.09995
Magnesium Sulfate (anhydrous) 0.0976876 0.0976876
Potassium Chloride 0.4 0.4
Sodium Bicarbonate 2.2
Sodium Chloride 6.46 6.46
Sodium Phosphate Monobasic (anhydrous) 0.504 0.504
Amino Acids
L-Alanine 0.01336 0.01336
L-Arginine • HCl 0.04214 0.04214
L-Asparagine • H2O 0.04503 0.04503
L-Aspartic Acid 0.01997 0.01997
L-Cysteine 0.02424 0.02424
L-Glutamic Acid 0.02207 0.02207
L-Glutamine 0.21915
Glycine 0.00751 0.00751
L-Histidine • HCl • H2O 0.02096 0.02096
Hydroxy-l-proline 0.01967 0.01967
L-Isoleucine 0.03936 0.03936
L-Leucine 0.03936 0.03936
L-Lysine • HCl 0.03654 0.03654
L-Methionine 0.01492 0.01492
L-Phenylalanine 0.01652 0.01652
L-Proline 0.01727 0.01727
L-Serine 0.02628 0.02628
L-Threonine 0.01787 0.01787
L-Tryptophan 0.00306 0.00306
L-Tyrosine • 2Na • 2H2O 0.0261 0.0261
L-Valine 0.01757 0.01757
Vitamins
Ascorbic Acid 0.0005625 0.0005625
p-Aminobenzoic Acid 0.001 0.001
D-Biotin 0.0002 0.0002
Choline Chloride 0.005 0.005
Folic Acid 0.01 0.01
myo-Inositol 0.036 0.036
Niacinamide 0.0005 0.0005
Nicotinic Acid 0.0005 0.0005
D-Pantothenic Acid (hemicalcium) 0.0002 0.0002
Pyridoxal • HCl 0.0005 0.0005
Pyridoxine • HCl 0.0005 0.0005
Riboflavin 0.0002 0.0002
Thiamine • HCl 0.0002 0.0002
Vitamin B12 0.002 0.002
Other
Peptone 0.6 0.6
D-Glucose 3 3
Glutathione (reduced) 0.0005 0.0005
Phenol Red • Na 0.011 0.011
Add
Sodium Bicarbonate 2.2
L-Glutamine 0.21915