Cell Culture

NCTC 109 Medium Formulation

Component N1140
[1x] g/L
N3262
g/L
Inorganic Salts
Calcium Chloride 0.2 0.2
Magnesium Sulfate (anhydrous) 0.1 0.1
Potassium Chloride 0.4 0.4
Sodium Acetate (anhydrous) 0.03 0.03
Sodium Bicarbonate 2.2
Sodium Chloride 6.8 6.8
Sodium Phosphate Monobasic (anhydrous) 0.122 0.122
Amino Acids
L-Alanine 0.03148 0.03148
L-Arginine • HCl 0.03116 0.03116
L-Asparagine • H2O 0.00919 0.00919
L-Aspartic Acid 0.00991 0.00991
L-Cysteine • HCl • H2O 0.28971
L-Cystine • 2HCl 0.01368 0.01368
L-Glutamic Acid 0.00826 0.00826
L-Glutamine 0.13573
Glycine 0.01351 0.01351
L-Histidine • HCl • H2O 0.02665 0.02665
Hydroxy-L-Proline 0.00409 0.00409
L-Isoleucine 0.01804 0.01804
L-Leucine 0.02044 0.02044
L-Lysine • HCl 0.03843 0.03843
L-Methionine 0.00444 0.00444
L-Ornithine • HCl 0.00941 0.00941
L-Phenylalanine 0.01653 0.01653
L-Proline 0.00613 0.00613
L-Serine 0.01075 0.01075
L-Threonine 0.01893 0.01893
L-Tryptophan 0.0175 0.0175
L-Tyrosine • 2Na • 2H2O 0.0237 0.0237
L-Valine 0.025 0.025
Vitamins
L-Ascorbic Acid 0.05 0.05
D-Biotin 0.000025 0.000025
Calciferol 0.00025 0.00025
Choline Chloride 0.00125 0.00125
Folic Acid 0.000025 0.000025
myo-Inositol 0.000125 0.000125
Menadione (sodium bisulfite) 0.00004 0.00004
Niacinamide 0.0000625 0.0000625
Nicotinic Acid 0.0000625 0.0000625
p-Aminobenzoic Acid 0.000125 0.000125
D-Pantothenic Acid (hemicalcium) 0.000025 0.000025
Pyridoxal • HCl 0.0000625 0.0000625
Pyridoxine • HCl 0.0000625 0.0000625
Retinol Acetate 0.00025 0.00025
Riboflavin 0.000025 0.000025
Thiamine • HCl 0.000025 0.000025
DL-α-Tocopherol Phosphate • 2Na 0.000025 0.000025
Vitamin B12 0.01 0.01
Other
L-Amino-n-Butyric Acid 0.00551 0.00551
Cocarboxylase 0.001 0.0001
Coenzyme A • Na 0.0025 0.0025
2'-Deoxyadenosine 0.01 0.01
2'-Deoxycytidine • HCl 0.01 0.01
2'-Deoxyguanosine • HCl 0.01 0.01
Flavin Adenine Dinucleotide • 2Na 0.001 0.001
D-Glucosamine • HCl 0.00385 0.00385
D-Glucose 1 1
Glucuronate • Na 0.0018 0.0018
D-Glucuronolactone 0.0018 0.0018
Glutathione • Na 0.02 0.02
5'-Methylcytosine • HCl 0.0001 0.0001
β-NAD 0.007 0.007
β-NADP • Na 0.001 0.001
Phenol Red • Na 0.02 0.02
L-Taurine 0.00418 0.00418
Thymidine 0.01 0.01
TWEEN 80 0.0125 0.0125
Uridine Triphosphate • Na 0.001 0.001
Add
L-Glutamine 0.13573
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