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Cell Culture

Waymouth MB 752/1 Medium Formulation

Component W1625
g/L
Inorganic Salts
Calcium Chloride 0.0906
Magnesium Chloride 0.11239
Magnesium Sulfate (anhydrous) 0.09767
Potassium Chloride 0.15
Potassium Phosphate Monobasic (anhydrous) 0.08
Sodium Chloride 6
Sodium Phosphate Dibasic (anhydrous) 0.3
Amino Acids
L-Arginine • HCl 0.075
L-Aspartic Acid 0.06
L-Cysteine • HCl • H2O 0.10029
L-Cystine • 2HCl 0.01955
L-Glutamic Acid 0.15
L-Glutamine 0.35
Glycine 0.05
L-Histidine • HCl • H2O 0.1641
L-Isoleucine 0.025
L-Leucine 0.05
L-Lysine • HCl 0.24
L-Methionine 0.05
L-Phenylalanine 0.05
L-Proline 0.05
L-Threonine 0.075
L-Tryptophan 0.04
L-Tyrosine • 2Na • 2H2O 0.0577
L-Valine 0.065
Vitamins
Ascorbic Acid • Na 0.0198
D-Biotin 0.00002
Choline Bitartrate 0.45352
Folic Acid 0.0004
myo-Inositol 0.001
Niacinamide 0.001
D-Pantothenic Acid (hemicalcium) 0.001
Pyridoxine • HCl 0.001
Riboflavin 0.001
Thiamine • HCl 0.01
Vitamin B12 0.0002
Other
D-Glucose 5
Glutathione (reduced) 0.015
Hypoxanthine 0.025
Phenol Red • Na 0.011
Add
Sodium Bicarbonate 2.24
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