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Cell Culture

Williams' Medium E Formulation

Component W1878
[1x] g/L
W4125
g/L
W4128
[1x] g/L
Inorganic Salts
Calcium Chloride 0.2 0.2 0.2
Cupric Sulfate • 5H2O 0.0000001 0.0000001 0.0000001
Ferric Nitrate • 9H2O 0.0000001 0.0000001 0.0000001
Magnesium Chloride • 4H2O 0.0000001 0.0000001 0.0000001
Magnesium Sulfate (anhydrous) 0.0977 0.0977 0.0977
Potassium Chloride 0.4 0.4 0.4
Sodium Bicarbonate 2.2 2.2
Sodium Chloride 6.8 6.8 6.8
Sodium Phosphate Monobasic (anhydrous) 0.122 0.122 0.122
Zinc Sulfate • 7H2O 0.0000002 0.0000002 0.0000002
Amino Acids
L-Alanine 0.09 0.09 0.09
L-Arginine (free base) 0.05 0.05 0.05
L-Asparagine • H2O 0.02 0.02 0.02
L-Aspartic Acid 0.03 0.03 0.03
L-Cysteine (free acid) 0.04 0.04 0.04
L-Cystine 0.02 0.02 0.02
L-Glutamic Acid 0.0445 0.0445 0.0445
L-Glutamine 0.292
Glycine 0.05 0.05 0.05
L-Histidine (free base) 0.015 0.015 0.015
L-Isoleucine 0.05 0.05 0.05
L-Leucine 0.075 0.075 0.075
L-Lysine • HCl 0.08746 0.08746 0.08746
L-Methionine 0.015 0.015 0.015
L-Phenylalanine 0.025 0.025 0.025
L-Proline 0.03 0.03 0.03
L-Serine 0.01 0.01 0.01
L-Threonine 0.04 0.04 0.04
L-Tryptophan 0.01 0.01 0.01
L-Tyrosine • 2Na • 2H2O 0.05045 0.05045 0.05045
L-Valine 0.05 0.05 0.05
Vitamins
Ascorbic Acid • Na 0.00227 0.00227 0.00227
D-Biotin 0.0005 0.0005 0.0005
Calciferol 0.0001 0.0001 0.0001
Choline Chloride 0.0015 0.0015 0.0015
Folic Acid 0.001 0.001 0.001
myo-Inositol 0.002 0.002 0.002
Menadione (sodium bisulfite) 0.00001 0.00001 0.00001
Niacinamide 0.001 0.001 0.001
D-Pantothenic Acid (hemicalcium) 0.001 0.001 0.001
Pyridoxal • HCl 0.001 0.001 0.001
Retinol Acetate 0.0001 0.0001 0.0001
Riboflavin 0.0001 0.0001 0.0001
Thiamine • HCl 0.001 0.001 0.001
DL-α-Tocopherol Phosphate • Na 0.00001 0.00001 0.00001
Vitamin B12 0.002 0.0002 0.0002
Other
D-Glucose 2 2 2
Glutathione (reduced) 0.00005 0.00005 0.00005
Methyl Linoleate 0.00003 0.00003 0.00003
Phenol Red • Na 0.0107 0.0107
Pyruvic Acid • Na 0.025 0.025 0.025
Add
L-Glutamine 0.292 0.292
Sodium Bicarbonate 2.2
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