Cell Culture

Primary Human Culture

Sigma is proud to offer a selection of media, many of which are serum-free, for the in vitro culture of human primary cells, such as keratinocytes, endothelial cells, and epithelial hepatocytes. These primary cell types have potential commercial value in a wide range of emerging technologies and therapeutic applications. Among these are: tissue engineering applications, such as wound healing and burn treatment; high throughput screening for drug toxicology and efficacy studies; gene therapy; pseudo-organs (e.g.. artificial liver) and as model cell systems for developing new therapeutic approaches to human diseases.




Endothelial-HUVEC Cells

   Human Umbilical Vein Endothelial Cell (HUVEC) Culture Medium
Human Umbilical Vein Endothelial Cell (HUVEC) Culture Companion Products and Supplements

Human Umbilical Vein Endothelial Cell (HUVEC) Culture Medium

Endothelial cells may be derived from any part of the vascular tree.  They may come from large and small veins and arteries, from capillaries, or from specialized vascular areas such as the umbilical vein of newborns, blood vessels in the brain or from vascularized solid tumors.  A convenient source of endothelial cells has been the human umbilical vein.  Hence, a number of media have been developed using cells from this source.  However, it is quite likely that, with little or no modification, these media will be useful for culturing endothelial cells from most, if not all, of the above sources.


Product # Product Name Description Application
C 1431 CS-C Medium
Serum Supplemented
  • Liquid
  • With 10% iron supplemented calf serum and L-glutamine
  • With HEPES and sodium bicarbonate buffers
  • Without phenol red
  • Sterile filtered
  • Endotoxin tested
  • Cell culture tested

Optimized to support the  proliferation of endothelial cells, such as human umbilical vein derived endothelial cells (HUVEC).

For best results, this medium should be supplemented with Endothelial Cell Growth Factor (E 9640) and Endothelial Cell Attachment Factor (E 9765)

Phenol red has been shown to interfere with the growth of some cells at clonal densities.

Use this medium when when growing cells at low densities.

C 1556 CS-C Medium
Without Serum
  • Liquid
  • With L-glutamine
  • With HEPES and sodium bicarbonate buffers
  • Without phenol red
  • Sterile filtered
  • Endotoxin tested
  • Cell culture tested

Optimized to support the  proliferation of endothelial cells, such as human umbilical vein derived endothelial cells (HUVEC).

For best results, this medium should be supplemented with up to 10% Fetal Bovine (F 2442) or Iron Supplemented Calf Serum (C 8056), Endothelial Cell Growth Factor (E 9640) and Endothelial Cell Attachment Factor (E 9765)

Phenol red has been shown to interfere with the growth of some cells at clonal densities.

 

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Human Umbilical Vein Endothelial Cell (HUVEC) Culture Companion Products and Supplements

The serum free culture of endothelial cells is supported by a series of products offered by Sigma.  The table below provides a partial list of Sigma products recommended for this purpose.  Also, see the "Human Umbilical Vein Endothelial Cell Culture Media" table above.  [This table may be found by using the hyperlink at the top of the page.]


Product # Product Name Description Application
E 9640 Endothelial Cell
Growth Factor
(100x)
  • Liquid
  • Prepared in Dulbecco's Phosphate Buffered Saline (DPBS), with 5 mg each of porcine heparin and HBGF-1
  • Sterile filtered
  • Mycoplasma tested
  • Endotoxin tested
  • Cell culture tested

Endothelial Cell Growth Factor stimulates the proliferation of endothelial cell types when used as a supplement with CS-C Medium (C 1431) which contains serum or CS-C Medium (C 1556) which requires serum to be added.

E 9765 Endothelial Cell
Attachment Factor
(ECAF)
  • Liquid
  • Contains gelatin (in HEPES buffer) to which attachment factors  derived from serum have been added
  • Sterile filtered
  • Mycoplasma tested
  • Endotoxin tested
  • Cell culture tested

Optimized to promote the attachment, spreading and polarity of endothelial cells (HUVEC).

Designed to be used with CS-C media (C 1431 & C 1556)

T 4299 Trypsin-EDTA Solution for Endothelial Cell Culture
  • Liquid
  • Contains 500 BAEE units porcine trypsin and 180 ug of EDTA74Na per mL in DPBS without calcium and magnesium
  • Powder negative for porcine parvovirus by 9 CFR
  • Sterile filtered
  • Mycoplasma tested
  • Cell culture tested

Useful for the dissociation of, or release of, adherent cells types, such as endothelial, epithelial, and fibroblast cells, from support surfaces.

 

T 7659 Trypsin Inhibitor
Defined
  • Liquid
  • Phosphate buffered saline solution containing (1x) 0.025% soybean trypsin inhibitor
  • pH approximately 7.4 at room temperature
  • Sterile filtered

Useful to inhibit the proteolytic action of Trypsin-EDTA (T 4299) after epithelial cells are released from a substrate or disaggregated. 

Chemically defined and free of animal derived components.  The absence of animal derived components  reduces the potential for contamination of cryopreserved cells with adventitious biological agents such as viruses.

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