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Cell Culture

CHO Kit 1

Faster, easier medium optimization is now in your hands

Researchers have long realized that individual Chinese hamster ovary (CHO) cell clones have highly specific nutritional needs in order to reach maximal cell growth and productivity. To help researchers optimize their CHO systems, we designed CHO Kit 1.

CHO Kit 1 (Product Code CH0001) from Sigma-Aldrich is the latest addition to the fastest growing line of animal component-free media in the industry. This novel kit provides researchers a convenient format in which they can rapidly screen multiple formulations to identify which formulations work best for their particular clones. It also provides detailed protocols utilizing Design-of-Experiment (DOE) methodology to allow researchers to take the three best performing media and perform blending experiments to further optimize media specific for their particular CHO systems.

CHO Kit 1 from Sigma-Aldrich provides the following benefits:

  • Six unique animal component-free media, including two chemically defined formulations, designed for maximum recombinant protein production in CHO cells
  • Media are designed to meet the raw material needs of the biopharmaceutical industry and are scalable under cGMP
  • Convenient format allows for rapid screening of multiple media to select optimal formulations
  • Simple yet powerful blending experiments (using DOE methodology) lets researchers quickly perform further media optimization experiments
  • Detailed instructions for various methods of data analysis are included
  • Don't want to perform the data analysis yourself? E-mail your normalized data to cellculture@sial.com and we'll do it for you! At no additional charge!

 

CH0001, CHO Kit 1 Components:


Product Code Description Pack Size CHO Kit 1 Components
C5467 CHO Medium, Animal Component-Free 1 L
C8862 CHO DHFR- Medium, Animal Component-Free 1 L
C4726 CHO Medium, Chemically Defined, Animal Component Free 1 L
C9737 CHO Medium 4, Animal Component-Free 1 L
C0363 CHO Medium 5, Animal Component-Free 1 L
C0488 CHO Medium 6, Chemically Defined, Animal Component-Free 1 L

 



Figure 1:

Following the initial screening of the 6 media contained in this kit, researchers can perform additional blending experiments with the three best performing media. This diagram and table represents the recommended blends for this initial set of experiments for media A, B, and C.



Figures 2 & 3:

Cell growth and viability in a recombinant CHO clone were compared under suspension conditions in blending experiments as described in Figure 1. Samples were taken daily to determine cell growth and recombinant protein productivity. The data demonstrates that these mixing experiments can identify several blends that give increased performance over the individual starting media.




Figures 4 & 5:
The data from the set of experiments shown in Figures 2 and 3 is presented in Figure 4 and is normalized to the performance of Medium A (A=100%) and ranked in terms of performance. Figure 5 contains the cell growth data presented in Figure 4, and demonstrates a simple way to visualize the performance of different mixtures. In this particular case, the best media mixtures for cell growth were located around points 4, 7, 8, and 10.




Figures 6 & 7:
Design-of-experiment software can be used for more precise data analysis and optimization. Figure 6 shows the contour plot for the cell growth data that was generated in this set of experiments. Figure 7 is a similar plot for the recombinant protein productivity data. These plots show that mixtures that perform well for one set of criteria might not perform as well for another set of criteria, emphasizing the need to assign relative importance values for each criterion that is being measured before deciding upon an optimal medium formulation.



Figure 8:

In this contour plot, we assigned equal importance values to cell growth and productivity. Using those guidelines resulted in a predicted optimal medium mixture consisting of 51% Medium A, 46% Medium B, and 3% Medium C.

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