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Cell Culture

MegaCell Media


We Took the Media You've Always Depended Upon and Turned It Into the Media You've Always Wanted.

Discover and save with Sigma's NEW MegaCell line of enriched cell culture media products designed to reduce Fetal Bovine Serum (FBS) supplementation to 3% or less.

We have optimized this classic media through fortification of a multitude of specially engineered supplements, growth factors and amino acids.

The result? The media you have always wanted: greater growth and performance while saving time and money.

 

MegaCell Formulation:
The MegaCell line of products is based on the classic basal medium formulations, which may be found in the Sigma Life Science Research catalog. MegaCell has been enriched with growth factors, amino acids, insulin, trace elements and non-essential amino acids. In addition, HEPES has been added to every bottle to increase buffering capacity. Serum supplementation was optimized at 3% for a broad range of cellular applications. However, MegaCell supplemented with 1-5% FBS performed very well.

 

 

View the complete MegaCell Brochure! (190 KB PDF)


M 3942 MegaCell DME:


MegaCell DME supplemented with 4 mM L-Glutamine and 3% FBS (Fetal Bovine Serum) was compared to DME supplemented with 4 mM L-Glutamine and 10% FBS. MDBK (Bovine Kidney) cells were plated without pre-adaptation to MegaCell DME at 5000 viable cells/cm2. The flasks were incubated at 37 °C with 5% CO2 and 95% air over a 4-day passage cycle. Cell growth and morphology were superior with MegaCell.

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M 4067 MegaCell MEM:


MegaCell MEM supplemented with 4 mM L-Glutamine and 3% FBS (Fetal Bovine Serum) was compared to MEM supplemented with 4 mM L-Glutamine and 10% FBS. Vero (Monkey African Green Kidney) cells were plated, without pre-adaptation to 5000 viable cells/cm2. The flasks were incubated at 37 °C with 5% CO2 and 95% air over a 4-day passage cycle. Cell growth and morphology were superior with MegaCell.

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M 4317 MegaCell MEM/F-12 HAM:


MegaCell MEM/F-12 supplemented with 4 mM L-Glutamine and 3% FBS (Fetal Bovine Serum) was compared to DME/F-12 supplemented with 4 mM L-Glutamine and 10% FBS. WI-38 (Human Caucasian Fetal Lung) cells were plated, without pre-adaptation to MegaCell MEM/F-12 at 5000 viable cells/cm2. The flasks were incubated at 37 °C with 5% CO2 and 95% air over a 4-day passage cycle. Cell growth and morphology were superior with MegaCell.

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M 3817 MegaCell RPMI-1640:


M 3817 MegaCell RPMI-1640 vs. Opti-MEM®:

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M 4192 MegaCell DME/F-12 HAM:

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MegaCell = Mega Options:

MegaCell gives you more options for serum supplementation. Without comprising cell growth or promotion, MegaCell supplemented with 6% Newborn Calf Serum or 10% Bovine Serum worked as well as the classic medium + 10% FBS.



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Common Applications for MegaCell + 3% Fetal Bovine Serum

MegaCell shows outstanding results on a wide variety of cell lines, including attached and suspension cells.

Cell Line
Seeding
Density
DME
M 3942
DME/F12
M 4192
MEM
M 4067
MEM/F12
M 4317
RPMI
M 3817
VERO (attached)
5000/cm2
-
+
+++
++
-
MDBK (attached)
5000/cm2
++++
+
+
+++
-
MRC-5 (attached)
5000/cm2
++
+++
++
+++
-
WI-38 (attached)
5000/cm2
+++
+++
+
+++
-
SP20 (suspension)
75000/ml
-
-
-
-
++++
HFN (suspension)
75000/ml
-
-
-
-
++++

Note: Number of "+" indicates strength of application data results.
Note: MegaCell is not recommended for 3T3 or MDCK cells.

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Product Name Product # 
MegaCell™ Minimum Essential Medium Eagle M4067
MegaCell™ Minimum Essential Medium/Nutrient Mixture F-12 Ham M4317
MegaCell™ Dulbecco's Modified Eagle's Medium M3942
MegaCell™ Dulbecco's Modified Eagle's Medium/Nutrient Mixture F-12 Ham M4192
MegaCell™ RPMI-1640 Medium M3817

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Sigma-Aldrich uses the MEGACELL trademark pursuant to an Agreement with Cortex Biochem, Inc.