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CompoZr® Cellular Reporter Cell Lines

Frequently Asked Questions

Why use CompoZr Cellular Reporter Cell Lines?

  • Easy visualization of live cells
    • Fluorescently tagged cytoskeletal proteins
  • Endogenous protein expression pattern
    • Tagged proteins driven by endogenous promoters
    • Upstream and downstream regulatory elements are preserved
    • Copy number is kept constant
  • Save time and money
    • Replace extensive antibody optimization
    • Eliminate staining artifacts
    • Reduce hands-on time for staining manipulations and molecular verification
  • Robust and reproducible results
    • Stable and heritable gene modification
    • Consistent results from experiment to experiment

Where to use CompoZr Cellular Reporter Cell Lines?

  • Observe perturbations to live cellular behavior
    • In protein localization and function
    • In gene regulation
    • High throughput and high content screening

Media Recommendations for CompoZr Cellular Reporters

U2OS cell lines

  • M9309 – McCoy’s 5A Medium Modified
  • F2442 – Fetal Bovine Serum
  • C6164 – Cell Freezing Media-DMSO

MCF10A cell lines

  • 51448C – Dulbecco’s Modified Eagle’s Medium/Ham’s Nutrient Mixture F-12
  • H1270 – Horse Serum
  • C8052 – Cholera Toxin (Vibrio cholerae)
  • I9278 – Insulin solution human (recombinant)
  • E9644 – Epidermal Growth Factor human
  • H6909 – Hydrocortisone solution
  • G3126 – L-glutamine
  • C6164 – Cell Freezing Media-DMSO

A549 cell lines

  • R0883 – RPMI-1640 Medium
  • F2442 – Fetal Bovine Serum
  • G7513 – L-Glutamine
  • C6164 – Cell Freezing Media-DMSO

SKOV cell lines

  • M8403 – McCoy's 5A Medium
  • F2442 – Fetal Bovine Serum
  • G7513 – L-Glutamine
  • C6164 – Cell Freezing Media-DMSO

Media Recommendations for CompoZr EGFR Biosensor

A549 Biosensor cell line

  • R0883 – RPMI-1640 Medium
  • F2442 – Fetal Bovine Serum
  • G7513 – L-glutamine
  • P9620 – Puromycin dihydrochloride
  • C6164 – Cell Freezing Media-DMSO

Media Recommendations for CompoZr Disease Models 

DLD1 cell lines

  • R5886 – RPMI-1640 Medium
  • F2442 – Fetal Bovine Serum
  • G7513 – L-glutamine
  • S8636 – Sodium pyruvate solution
  • C6164 – Cell Freezing Media-DMSO

A549 cell lines

  • N4888 – F-12 Ham’s Nutrient Mixture
  • F4135 – Fetal Bovine Serum
  • G7513 – L-glutamine
  • C6164 – Cell Freezing Media-DMSO

SW48 cell lines

  • D5671 – Dulbecco’s Modified Eagle’s Medium – high glucose
  • F4135 – Fetal Bovine Serum
  • G7513 – L-glutamine
  • S8636 – Sodium pyruvate solution
  • C6164 – Cell Freezing Media-DMSO

MCF10A cell lines

  • 51448C – Dulbecco’s Modified Eagle's Medium/Ham’s Nutrient Mixture F-12
  • H1270 – Horse Serum
  • C8052 – Cholera Toxin (Vibrio cholerae)
  • I9278 – Insulin solution human (recombinant)
  • E9644 – Epidermal Growth Factor human
  • H6909 – Hydrocortisone solution
  • G3126 – L-glutamine
  • C6164 – Cell Freezing Media-DMSO

For more information on these or any of our ZFN-modified cell lines please contact us.