Cell Design Studio Highlighted Projects

Click here to learn about some of our highlighted projects.
 

 Cardiomyocytes Beating

ZFNs were used to target the endogenous NKX2-5 allele and introduce a GFP lineage marker following a ribosomal skip sequence in iPSCs. GFP was not expressed following clonal selection, but was robustly expressed following directed differentiation into cardiomyocytes, in which NKX2-5 is expressed. The video shows beating cardiomyocytes expressing GFP under the control of the NKX2-5 locus.

 STAT1 and STAT3 Localizing to the Nucleus

ZFNs were used to target the endogenous STAT1 and STAT3 alleles in A549 cells to produce GFP-tagged STAT1 and RFP-tagged STAT3, respectively. Here, the modified cells have been stimulated with IFN-γ and IL-6 and imaged over time to monitor the nuclear localization of STAT1 (green) and STAT3 (red), shown on the right. The left panel shows a DIC image of the same field with DAPI nuclear stain in blue.

 Tubulin in Response to Paclitaxel

ZFNs were used to target the endogenous TUBA1B locus and produce an RFP-tagged TUBA1B allele in MCF10A cells. When indicated, the modified cells were treated with paclitaxel to inhibit microtubule dynamics, visualized by the RFP-α-tubulin. The left panel shows a DIC image of the same field.