Biological Buffers


Synonym: β-Aminoisobutyl alcohol, 2-Amino-2-methyl-1-propanol

Used in an efficient synthesis of 2-oxazolidinones via carbonylation with CO in the presence of salen-cobalt catalysts. Used to derivatize carboxylic acids for GC analysis1 and to synthesize 2-oxazolines2 for further transformations3. Determination of alkaline phosphatase4,5 Stabilizes the Calmagite reagent in the automated measurement of magnesium in serum and urine6.
Useful pH Range 9.0 - 10.5
Working concentration range no reliable information


        Comparison of products by grade or quality level
Name CAS Molecular Formula Mol Wt Reagent Grades Life Science Grades PharmaGrade  Solutions
AMP 124-68-5 C4H11NO 89.14 A65182 8.01465 08581 A9199 08578    
AMP hydrochloride 1185-53-1 C4H12ClNO 125.60   8.14099    


Interaction Comments

AMP has a low melting point (18 - 26°C) and has to be liquified at approx. 35°C to prepare a buffer solution. The liquified AMP has a high viscosity, which makes its handling more difficult4.
If this buffer solution is stored at room temperature and protected from the CO2 from the air, it is stable for approx. one month4.
Slight interaction with Ni, Cu, Zn, Cd and Pb7


Literature References

  1. J. Chromatogr. A. 673, 101, (1994)
  2. Tetrahedron 49, 9353, (1993)
  3. 3. J. Am. Chem. Soc. 114, 1010, (1992)
  4. J.-P. Bretaudiere and T. Spillman, H.U. Bergmeyer, ed. Methods of Enzymatic Analysis 3rd ed., Weinheim, Fed.Rep.Ger. 4, 75, (1984)
  5. H J Derks et. al. Elimination of inhibitors of alkaline phosphatase from 2-amino-2-methyl-1-propanol. Clinical Chemistry, 27(2), 318-321 (1981).
  6. J Savory et. al. Stabilization of the calmagite reagent for automated measurement of magnesium in serum and urine. Clinical Chemistry, 31(3), 487-488 (1985).
  7. Ferreira CM, Pinto IS, Soares, EV, Soares HM. 2015 Royal Society of Chemistry 30989- 31003