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Biological Buffers

AMPD

Synonym: 2-Amino-2-methyl-1,3-propanediol, Ammediol
 

AMPD is a buffer component in a SDS-gradient gel electrophoresis system that separates polypeptides in the molecular weight range of 1500 to 100,0001. Used as a spacer in isotachophoresis of proteins2,3. Also used as a buffer for the determination of alkaline phosphatase activity4.
Useful pH Range 7.8 - 9.7
Working concentration range 50-100mM6,7

 

        Comparison of products by grade or quality level
Name CAS Molecular Formula Mol Wt Reagent Grades Life Science Grades PharmaGrade  Solutions
AMPD 115-69-5 C4H11NO2 105.14 A9754 08569 8.01464    

 

Interaction Comments
 

Strong interaction with Cu; weak interaction with Ni7

 

Literature References

  1. A sodium dodecyl sulfate-gradient gel electrophoresis system that separates polypeptides in the molecular weight range of 1500 to 100,000. D Bothe et. al. Analytical Biochemistry, 151(1), 49-54 (1985)
  2. Spacer performance in the cationic isotachophoresis of proteins. F S Stover Audio and Electroacoustics Newsletter, IEEE, 470(1), 201-208 (1989).
  3. Experimental and theoretical description of the isotachophoretic behavior of serum albumin. W Thormann and R A Mosher. Electrophoresis, 11(4), 292-298 (1990).
  4. Stability of isoenzymes of alkaline phosphatase in various buffer systems. K Jung et. al. Enzyme, 24(1), 18-22 (1979).
  5. Ferreira CM, Pinto IS, Soares, EV, Soares HM. 2015 Royal Society of Chemistry 30989- 31003
  6. Horikoshi K. Alkaliphile; Harwood Academic Publishers, 1999
  7. Gomori, G., Buffers in the range of pH 6.5 to 9.6, Proc. Soc. Exp. Biol. Med. 1946(62; 33)