Custom siRNA Oligos

N-TER Nanoparticle siRNA

Catalog Number N0788

 

Product Description
The N-TER™ Nanoparticle siRNA Transfection System is a peptide-based system for use in the transient knockdown of eukaryotic gene expression.1 The N-TER Peptide binds siRNAs non-covalently, forming a nanoparticle.2 This nanoparticle interacts directly with lipids on the surface of the plasma membrane,1,2 allowing the nanoparticle to diffuse across the cell membrane and deliver the siRNA directly to the cytoplasm. Unlike most lipid-based transfection methods, this novel delivery mechanism bypasses the endosomal pathway,1,3,4 eliminating possible compartmentalization and degradation of the siRNA.1,5

Proligo

Table 1
Cell lines for which the N-TER Nanoparticle siRNA Transfection System have been experimentally validated

Cell Type Source Tissue
3T3-L1, differentiated adipocyte mouse embryonic fibroblast cell line
A2780 human ovarian carcinoma cell line
A549 human lung carcinoma cell line
ASPC-1 human pancreatic carcinoma cell line
Astrocyte human neuronal primary cell
Astrocytoma human neuronal astrocytoma cell line
BSMC human bronchial smooth muscle primary cell
C2C12, differentiated myocyte mouse myoblastoma line
HeLa human cervical adenocarcinoma cell line
HepG2 human hepatocarcinoma cell line
HT-29 human colorectal adenocarcinoma cell line
HUVEC human umbilical vein epithelial primary cell
MCF7 human breast adenocarcinoma cell line
MDA-MB-231 human breast adenocarcinoma cell line
NHEK-AD human adult keratinacyte primary cell
Raw264.7 mouse macrophage cell line
SW620 human colorectal adenocarcinoma cell line
U-87 MG human brain glioblastoma cell line

Reagents included in each kit:

Product #  Product Name
N0538 N-TER Peptide, 120 µL
N0413 N-TER Buffer, 4 mL

The amounts provided are sufficient for up to 200 reactions, assuming a final siRNA concentration of 30 nM in a final assay volume of 160 µL.

Storage/Stability
If stored properly, shelf life is 6 months from the date of shipment. The N-TER Peptide should be stored at –20 °C, and the N-TER Buffer may be stored at room temperature.

References

  1. Simeoni, F., et al., Insight into the mechanism of the peptide-based gene delivery system MPG: implications for delivery of siRNA into mammalian cells. Nucleic Acids Res., 31, 2717-2724 (2003).
  2. Morris, M.C., et al., A novel potent strategy for gene delivery using a single peptide vector as a carrier. Nucleic Acids Res., 27, 3510-3517 (1999).
  3. Friend, D.S., et al., Endocytosis and intracellular processing accompanying transfection mediated by cationic liposomes. Biochim. Biophys. Acta., 1278, 41-50 (1996).
  4. Xu, Y. and Szoka, F.C., Mechanism of DNA release from cationic liposome/DNA nanoparticles used in cell transfection. Biochemistry, 35, 5616-5623 (1996).
  5. Morris, M.C., et al., A new peptide vector for efficient delivery of oligonucleotides into mammalian cells. Nucleic Acids Res., 25, 2730-2736 (1997).

The N-TER Nanoparticle siRNA Transfection System is manufactured and distributed by Sigma-Aldrich under license from CNRS (France). N-TER is a trademark of Sigma-Aldrich Biotechnology

* This product is available in combination with siRNA orders.