Attention:

Certain features of Sigma-Aldrich.com will be down for maintenance the evening of Friday August 18th starting at 8:00 pm CDT until Saturday August 19th at 12:01 pm CDT.   Please note that you still have telephone and email access to our local offices. We apologize for any inconvenience.

Functional Genomics & RNA

Trophoblast Protocol

Sigma® has prepared product lists for commonly used protocols from Cold Spring Harbor Protocols.

View the entire protocol on BioSupplyNet.com.

View the entire protocol "Culturing Trophoblast Stem (TS) Cell Lines" at BioSupplynet.com


Janet Rossant
This protocol was adapted from "Isolation and Culture of Blastocyst-Derived Stem Cell Lines," Chapter 8 (Protocol 8, provided by the laboratory of Janet Rossant), in Manipulating the Mouse Embryo, 3rd edition, by Andras Nagy, Marina Gertsenstein, Kristina Vintersten, and Richard Behringer. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, USA, 2003.

ABSTRACT
Recently, culture conditions have been established for a second blastocyst-derived cell line, trophoblast stem (TS) cells, in addition to embryonic stem (ES) cells. This protocol describes a method for culturing TS cell lines. These cells can then be used to study trophoblast differentiation and placental function.
Manipulating the Mouse Embryo - Third Edition
Buy this book,
Z700401

Products Available for this Protocol
Protocol Material Description Product # Product Name Add to Cart
Dimethylsulfoxide D2650 Dimethyl sulfoxide, liquid (temperature dependent), ≥99.7%, Hybri-Max™, sterile-filtered, hybridoma tested
Heparin H4784 Heparin sodium salt, ~170 USP units/mg
Gelatin (Swine Skin) G2500 Gelatin from porcine skin, Type A
Trypsin-EDTA T3924 Trypsin-EDTA solution, 1 ×, 0.5 g porcine trypsin and 0.2 g EDTA • 4Na per liter of Hanks' Balanced Salt Solution with phenol red., sterile-filtered, cell culture tested
BSA fraction V A4503 Albumin from bovine serum, Cohn V fraction, ≥96% (agarose gel electrophoresis), lyophilized powder
PBS P5368 Phosphate buffered saline, pH 7.4, Biotechnology Performance Certified
Mitomycin C stock M4287 Mitomycin C from Streptomyces caespitosus, powder, cell culture tested
Dulbecco’s modified Eagle’s medium (DMEM) D6046 Dulbecco’s Modified Eagle’s Medium - low glucose, With 1000 mg/L glucose, L-glutamine, and sodium bicarbonate, liquid, sterile-filtered, cell culture tested
Fetal bovine serum (FBS) F2442 Fetal Bovine Serum, USA origin, sterile-filtered, cell culture tested, hybridoma tested
Sodium pyruvate P5280 Sodium pyruvate, cell culture tested, insect cell culture tested, ≥99%, powder
β-mercaptoethanol M3148 2-Mercaptoethanol, for molecular biology, ≥98% (GC/titration)
L-glutamine G6392 L-Glutamine, γ-irradiated, cell culture tested
Penicillin and Streptomycin P4333 Penicillin-Streptomycin, 100 ×, liquid, stabilized, sterile-filtered, cell culture tested
RPMI 1640 R8758 RPMI-1640 Medium, with L-glutamine and sodium bicarbonate, liquid, sterile-filtered, cell culture tested

Back to Top
View all available product lists for Cold Spring Harbor Laboratory Protocols

Product Association Disclaimer: The Sigma-Aldrich products listed for this specific protocol were selected either to match or to supplement the products listed within the actual protocol. The products/reagents from Sigma-Aldrich have been qualified for usage, but may not have been validated for this specific application. Please refer to the detailed product description on the usage of specific products of interest.