Lentivirus Cell Line

Using lentivirus as a means to deliver shRNAs has become standard practice in many labs exploring RNAi. Infection (transduction) with VSV-G pseudotyped lentivirus allows for simple, efficient delivery into a wide variety of cell types, including: non-dividing, primary, and stem cells. In the few instances when a particular cell type is difficult to transduce, there are multiple methods to enhance delivery, such as: Spinoculation or magnetic nanoparticle beads (ExpressMag™ Transduction System). 

Table Overview
The table below is designed to aid in your lentivirus transduction experiments when working with a given cell line. All validated cell lines are a result of researchers using the RNAi Consortium (TRC) shRNA library delivered via lentivirus. If you fail to find your cell line of interest, it is suggested to identify similar cell lines that may act as a good starting point for your own research. Transduction conditions are presented in one of two ways on this table.

Many cells are directly linked to a PubMed literature reference that can be accessed by left clicking anywhere within the row or by clicking the NCBI Link cell. There may be multiple references to a single cell type. It is encouraged to review multiple references to best gauge the best transduction conditions for your model.

Some cells will have specific transduction conditions already populated within the table. These conditions were experimentally determined from either the Broad Institute, Sigma® R&D or external collaborators. While these conditions worked well for a given researcher, they are meant to act as a starting point in your own work.

If the source is the Sigma MISSION® Team or the TRC-Broad Institute, then the virus titer was between 10e6 and 10e7.


Cell Name PubMed ID or Source Organ / Tissue Cancer / Cell Type Organism Seeding Density (1000 cells / cm^2) MOI (x) or Virus Vol. (µl/1000 cells) Puromycin (µg/ml) Notes
Phoenixamphotropic 18006659 - - - - - - -
PSR-1E 17360431 - - - - - - -
SC21 18372920 - - - - - - -
siCandsiPML2 16873256 - - - - - - -
Macrophages 19237602 Blood - H. sapiens - - - -
CL22 18372920 Epidermis - M. musculus - - - -
Gingival epithelial cells 18624948 Epithelial - - - - - -
HF 16873256 Foreskin - H. sapiens - - 2.0 -
HaCAT 18794124 Keratinocyte - H. sapiens - - 10.0 -
BE R 18070930 Kidney - M. auratus - - - -
LLC-PK1 18474214 Kidney - S. domestica - - - -
MDCK 18172008 Kidney - C. Lupus familliarus - - 4.0 -
HMECtert 17360431 Mammary - H. sapiens - - - -
5A97 17936323 Mesothelium - H. sapiens - - - -
5ADL 17442711 Mesothelium - H. sapiens - - - -
Mouse Muscle Cells 18552838 Muscle - M. musculus - - - -
astrocytoma samples 18398503 Nervous system - - - - - -
embryonic cortical neuroepithelium 17409236 Nervous system - M. musculus - - - -
MN 17409236 Nervous system - - - 10 x - -
GH3 17706597 Pituitary - R. norvegicus - - - -
CE 18441300 Retina - H. sapiens - - - -
BxPC-3 18316571 Pancreas, epithelial Adenocarcinoma H. sapiens - 10 x - -
BxPC-3 18403640 Pancreas, epithelial Adenocarcinoma H. sapiens - - - -
MCF-7 17384642 Breast Adenocarcinoma H. sapiens - - - -
MCF-7 17535848 Breast Adenocarcinoma H. sapiens - - - -
MCF-7 17574021 Breast Adenocarcinoma H. sapiens - - - -
MCF-7 18948598 Breast Adenocarcinoma H. sapiens - - - -
MCF-7 19015320 Breast Adenocarcinoma H. sapiens - - - -
MCF-7 19061500 Breast Adenocarcinoma H. sapiens - - - -
MCF-7 Sigma MISSION Team Breast Adenocarcinoma H. sapiens 47.5 2 x 1.0 -
MCF-7 TRC-Broad Institute Breast Adenocarcinoma H. sapiens 3.1 8.0 µl/1000 cells 2.0 Utilize Spinfection
MDA-MB-231 18403640 Breast Adenocarcinoma H. sapiens - - - -
MDA-MB-231 19014680 Breast Adenocarcinoma H. sapiens - - - -
MDA-MB-231 Sigma MISSION Team Breast Adenocarcinoma H. sapiens 62.5 1 x 0.5 -
MDA-MB-468 18403640 Breast Adenocarcinoma H. sapiens - - - -
COLO205 17957250 Colon Adenocarcinoma H. sapiens - - - -
DLD-1 17906625 Colon Adenocarcinoma H. sapiens - - - -
DLD-1 18794900 Colon Adenocarcinoma H. sapiens - - - -
HT-29 17141160 Colon Adenocarcinoma H. sapiens - 3 x 2.0 -
HT-29 18403640 Colon Adenocarcinoma H. sapiens - - - -
HT-29 19014680 Colon Adenocarcinoma H. sapiens - - - -
HT-29 Sigma MISSION Team Colon Adenocarcinoma H. sapiens 32.0 3 x 2.0 -
SW480 19014680 Colon Adenocarcinoma H. sapiens - - - -
SK-HEP-1 18403640 Liver Adenocarcinoma H. sapiens - - - -
Calu-3 15731348 Lung Adenocarcinoma H. sapiens - - - -
H1703 18427549 Lung Adenocarcinoma H. sapiens - - - -
NCI-H838 18403640 Lung Adenocarcinoma H. sapiens - - - -
PC9 18711136 Lung Adenocarcinoma H. sapiens - - - -
PC9 19079597 Lung Adenocarcinoma H. sapiens - - - -
PC9NSCLC 18591665 Lung Adenocarcinoma H. sapiens - - - -
ES-2 17942676 Ovary Adenocarcinoma H. sapiens - - - -
OVCAR-3 17311912 Ovary Adenocarcinoma H. sapiens - - - -
OVCAR-3 17942676 Ovary Adenocarcinoma H. sapiens - - - -
SKOV-3 15252144 Ovary Adenocarcinoma H. sapiens - - - -
SKOV-3 17942676 Ovary Adenocarcinoma H. sapiens - - - -
SKOV-3 17942676 Ovary Adenocarcinoma H. sapiens - - - -
SKOV-3 Sigma MISSION Team Ovary Adenocarcinoma H. sapiens 31.3 15 x - -
SKOV-3 Sigma MISSION Team Ovary Adenocarcinoma H. sapiens 31.3 15 x - ExpressMag Enhanced
SK-OV-3 18403640 Ovary Adenocarcinoma H. sapiens - - - -
ACHN 18948597 Renal Adenocarcinoma H. sapiens - - - -
NCI-H820 19079597 Lung Adenocarcinoma, papillary H. sapiens - - - -
D54 17707275 Nervous system Astrocytoma H. sapiens - - 1.0 Polybrene
NCH492 18398503 Nervous system Astrocytoma, pilocytic H. sapiens - - 2.0 Polybrene
GM05823 18922873 - Ataxia telangiectasia H. sapiens - - - -
HS578T TRC-Broad Institute Breast Breast Cancer H. sapiens 3.1 3.8 µl/1000 cells 2.0 Utilize Spinfection
16HBE 18204074 Lung Bronchial Epithelial H. sapiens - - 1.0 -
16HBE 18204074 Lung Bronchial Epithelial H. sapiens - - - -
CRC 17906625 Colorectal Cancer H. sapiens - - 1.0 -
J82 18403640 Bladder Carcinoma H. sapiens - - - -
T24 18403640 Bladder Carcinoma H. sapiens - - - -
MDA-MB-453-I4 18353781 Breast Carcinoma H. sapiens - - - -
MMC Sigma MISSION Team Breast Carcinoma M. musculus 62.5 4 x 8.0 -
T47D 19061500 Breast Carcinoma H. sapiens - - - -
T47D TRC-Broad Institute Breast Carcinoma H. sapiens 3.1 6.0 µl/1000 cells 2.0 Utilize Spinfection
ZR-75-1 18757432 Breast Carcinoma H. sapiens - - - DNA Transient
Co-115 17906625 Colon Carcinoma H. sapiens - - - -
HCT116 17967892 Colon Carcinoma H. sapiens - - - -
HCT116 18403640 Colon Carcinoma H. sapiens - - - -
HCT116 18562296 Colon Carcinoma H. sapiens - - - -
HCT116 18794900 Colon Carcinoma H. sapiens - - - -
HCT116 19014680 Colon Carcinoma H. sapiens - - - -
HCT116 Sigma MISSION Team Colon Carcinoma H. sapiens 30.0 5 x 1.0 -
RKO 18403640 Colon Carcinoma H. sapiens - - - -
RKO 18948598 Colon Carcinoma H. sapiens - - - -
A431 15731348 Epithelial Carcinoma H. sapiens - - - -
A431 Sigma MISSION Team Epithelial Carcinoma H. sapiens 75.0 5 x 0.5 -
A498 18948597 Kidney Carcinoma H. sapiens -   - -
Hepa1-6 Sigma MISSION Team Liver Carcinoma M. musculus 32.0 3 x 2.0 -
HepG2 17449864 Liver Carcinoma H. sapiens - 10 x - -
HepG2 17687072 Liver Carcinoma H. sapiens - - - -
HepG2 17993646 Liver Carcinoma H. sapiens - - 2.0 Polybrene
HepG2 18403640 Liver Carcinoma H. sapiens - - - -
HepG2 Sigma MISSION Team Liver Carcinoma H. sapiens 32.0 3 x 2.0 -
A549 15731348 Lung Carcinoma H. sapiens - - - -
A549 16982682 Lung Carcinoma H. sapiens - - - -
A549 17535848 Lung Carcinoma H. sapiens - - - -
A549 18337815 Lung Carcinoma H. sapiens - - - -
A549 18337823 Lung Carcinoma H. sapiens - - - -
A549 18403640 Lung Carcinoma H. sapiens - - - -
A549 18768981 Lung Carcinoma H. sapiens - - - -
A549 18948595 Lung Carcinoma H. sapiens - - - -
A549 Sigma MISSION Team Lung Carcinoma H. sapiens 30.0 5 x 2.0 -
A549 Sigma MISSION Team Lung Carcinoma H. sapiens 46.9 5 x 1.0 -
A549 TRC-Broad Institute Lung Carcinoma H. sapiens 1.6 6.0 µl/1000 cells 2.0 -
LLC-1 Sigma MISSION Team Lung Carcinoma M. musculus 46.9 6 x 2.0 -
NSCLC 16906227 Lung Carcinoma - - - - -
NSCLC 19079597 Lung Carcinoma - - - - -
TSA 18559453 Mammary gland Carcinoma M. musculus - - - -
TOV112D 17942676 Ovary Carcinoma H. sapiens - - - -
8988T 19050074 Pancreas Carcinoma H. sapiens - - - -
MiaPaCa 18089810 Pancreas Carcinoma H. sapiens - - - -
MiaPaCa2 17114012 Pancreas Carcinoma H. sapiens - - - -
MiaPaCa2 17178855 Pancreas Carcinoma H. sapiens - - 3.0 -
MiaPaCa2 18403640 Pancreas Carcinoma H. sapiens - - - -
DU145 17709381 Prostate Carcinoma H. sapiens - - - -
DU145 18089825 Prostate Carcinoma H. sapiens - - - -
DU145 18403640 Prostate Carcinoma H. sapiens - - - -
DU145 18838960 Prostate Carcinoma H. sapiens - - - -
PC3 17574021 Prostate Carcinoma H. sapiens - - - -
PC3 18089825 Prostate Carcinoma H. sapiens - - - -
PC3 18403640 Prostate Carcinoma H. sapiens - - - -
PC3 18838960 Prostate Carcinoma H. sapiens - - - -
LNCaP 18089825 Prostate Carcinoma H. sapiens - - - -
LNCaP 18838960 Prostate Carcinoma H. sapiens - - - -
LNCaP Sigma MISSION Team Prostate Carcinoma H. sapiens 62.5 5 x 2.0 -
786-O 18403640 Renal Carcinoma H. sapiens - - - -
786-O 18948598 Renal Carcinoma H. sapiens - - - -
786-O TRC-Broad Institute Renal Carcinoma H. sapiens 1.9 3.3 µl/1000 cells 3.5 -
HSC-5 17535848 Skin Carcinoma H. sapiens - - - -
NCI-H647 18948598 Lung Carcinoma, adenosquamous H. sapiens - - - -
H358 15731348 Lung Carcinoma, Bronchioalveolar H. sapiens - - - -
NCI-H358 18948591 Lung Carcinoma, bronchioalveolar H. sapiens - - - -
CaSki 18948591 Cervix Carcinoma, epidermoid H. sapiens - - - -
OECM-1 18213732 Keratinocyte Carcinoma, epidermoid H. sapiens - - - -
Calu-1 18948595 Lung Carcinoma, epidermoid H. sapiens - - - -
HeLa 15737938 Cervix Carcinoma, epitheloid H. sapiens - - - -
HeLa 16916793 Cervix Carcinoma, epitheloid H. sapiens - - - -
HeLa 16998475 Cervix Carcinoma, epitheloid H. sapiens - - - -
HeLa 17386266 Cervix Carcinoma, epitheloid H. sapiens - - - -
HeLa 17724024 Cervix Carcinoma, epitheloid H. sapiens - - - -
HeLa 18403640 Cervix Carcinoma, epitheloid H. sapiens - - - -
HeLa 18564371 Cervix Carcinoma, epitheloid H. sapiens - - - -
HeLa 18768981 Cervix Carcinoma, epitheloid H. sapiens - - - -
HeLa 18948591 Cervix Carcinoma, epitheloid H. sapiens - - - -
HeLa 19017811 Cervix Carcinoma, epitheloid H. sapiens - - - -
HeLa 19293187 Cervix Carcinoma, epitheloid H. sapiens - - - -
HeLa Sigma MISSION Team Cervix Carcinoma, epitheloid H. sapiens 32.0 3 x 2.0 -
WRO 17639054 Thyroid Carcinoma, Follicular H. sapiens - - - Polybrene
KATO III 18381441 Stomach Carcinoma, gastric H. sapiens - - 8.0 Polybrene
HuH-7 17272514 Liver Carcinoma, Hepatocellular H. sapiens - - 1.0 -
T84 18974311 Lung Carcinoma, metastatic H. sapiens - - - -
H3255 19079597 Lung Carcinoma, NSCLC H. sapiens - - - -
H3255 Sigma MISSION Team Lung Carcinoma, NSCLC H. sapiens 25.0 10 x 1.5 -
SAS HNSCC 18213732 - Carcinoma, squamos H. sapiens - - - -
SW579 18403640 Thyroid Carcinoma, squamous H. sapiens - - - -
SiHa 18948597 Cervix Carcinoma, squamous cell H. sapiens - - - -
HEp-2 16873258 Larynx Carcinoma, squamous cell H. sapiens - - - -
HEp-3 19279115 Larynx Carcinoma, squamous cell H. sapiens - - - -
MOSER-S 17565986 Colon Colorectal cancer H. sapiens - - - Polybrene
BM-DC 12649500 Bone Marrow Dendritic cells - - 4500 x 1.0 Polybrene
MES23.5 18980610 Nervous system Dopaminergic R. norvegicus - - - -
P6 17828296 Fibroblast, embryo Embryonic M. musculus - - - -
REF 18619965 Fibroblast, embryo Embyryonic R. norvegicus - - - DNA Transient
HUVEC 16828724 Umbilicus Endothelial cells H. sapiens - 100 x - Polybrene
HUVEC 17535848 Umbilicus Endothelial cells H. sapiens - - - -
HUVEC 18602101 Umbilicus Endothelial cells H. sapiens - - - -
HMVEC Sigma MISSION Team Endothelial Endothelial, microvascular H. sapiens 46.9 10 x 2.0 -
HMLE 18483246 Breast Epithelial H. sapiens - - - -
HMLER 18483246 Breast Epithelial H. sapiens - - - -
HC11 18836481 Mammary Epithelial M. musculus - - - -
HMEC 17535848 Mammary Epithelial H. sapiens - - - -
HMEC 17535848 Mammary Epithelial H. sapiens - - - -
HMEC 17574021 Mammary Epithelial H. sapiens - - - -
IOSE80 17942676 Ovary Epithelial M. musculus - - - -
TEC TRC-Broad Institute Thymus Epithelial H. sapiens 3.1 2.0 µl/1000 cells 2.0 Utilize Spinfection
hTERT-HME1 19112104 Mammary gland, breast Epithelial immortalized with hTERT H. sapiens - - - -
MCF-12A 17360431 Breast Epithelial spontaneous immortilization H. sapiens - - - -
MCF-10A 17360431 Breast Epithelial, non-tumorigenic H. sapiens - - - -
MCF-10A 18094039 Breast Epithelial, non-tumorigenic H. sapiens - - - -
MCF-10A 17060456 Breast Epithelial, non-tumorigenic H. sapiens - - - -
MCF-10A 18413746 Breast Epithelial, non-tumorigenic H. sapiens - - - -
MCF-10A 19112533 Breast Epithelial, non-tumorigenic H. sapiens - - - -
IEC-6 19014680 Small intestine Epithelioid R. norvegicus - - - -
HD3 18703050 - Erythroblast G. domesticus - 5 x 1.0 -
TF-1 17942397 Bone Marrow Erythroleukemia H. sapiens - - - -
HEL 17347684 Embryonic Kidney Erythroleukemia, AML M6 H. sapiens - - - -
F02-98 18922873 - Fibroblast H. sapiens - - - -
FR3t3 17692050 - Fibroblast R. norvegicus - - - -
MCH 58 18940309 - Fibroblast H. sapiens - - - -
MCH 58 18940309 - Fibroblast H. sapiens - - - -
MCF-7-ras 15882617 Breast Fibroblast H. sapiens - - - -
CL1 18838960 Embryo Fibroblast C. griseus - - - -
BHK-21 18768981 Kidney Fibroblast M. auratus - - - -
NIH-3T3 19293187 Embryo, NIH Swiss Fibroblast, adherent monolayer M. musculus - - - -
MRC-5 17581253 Lung, embryonic Fibroblast-like H. sapiens - - - -
WI-38 18006659 Lung, fetal Fibroblast-like H. sapiens - - - -
WI-38 18006659 Lung, fetal Fibroblast-like H. sapiens - - - -
WI-38 18948595 Lung, fetal Fibroblast-like H. sapiens - - - -
COS-7 17550976 Kidney Fibroblast-like C. aethiops - - - DNA Transient
COS-7 17684014 Kidney Fibroblast-like C. aethiops - - - -
HFFF2 18922870 Foreskin Fibroblasts H. sapiens - - - -
HLF 12796781 Lung Fibroblasts H. sapiens - - - -
IMR-90 17082179 Lung Fibroblasts H. sapiens - - - -
IMR-90 17082179 Lung Fibroblasts H. sapiens - - - -
IMR-90 18006659 Lung Fibroblasts H. sapiens - - - -
IMR-90 18006659 Lung Fibroblasts H. sapiens - - - -
MRC-5 Sigma MISSION Team Lung, embryonic Fibroblasts H. sapiens 20.8 1 x 2.0 -
2091 17082179 Foreskin Fibroblasts, early passage H. sapiens - - - -
C3H10T1/2 17183530 Fibroblast Fibroblasts, embryo M. musculus - - 5.0 -
MEF 16951254 Connective Fibroblasts, embryonic M. musculus - - 1.0 -
MEF 17386266 Connective Fibroblasts, embryonic M. musculus - - - -
MEF 18250155 Connective Fibroblasts, embryonic M. musculus - - - -
MEF 19112104 Connective Fibroblasts, embryonic M. musculus - - - -
MEF 19150980 Connective Fibroblasts, embryonic M. musculus - - - -
MEF Sigma MISSION Team Connective Fibroblasts, embryonic M. musculus 46.9 16 x 3.0 -
MEF Sigma MISSION Team Connective Fibroblasts, embryonic M. musculus 46.9 8 x 3.0 ExpressMag Enhanced
MCF-10A-2A 17360431 Breast Fibrocystic disease H. sapiens - - - -
HT-1080 18518968 Fibroblast Fibrosarcoma H. sapiens - - - -
HT-1080 18518968 Fibroblast Fibrosarcoma H. sapiens - - - Polybrene
HT-1080 19293187 Fibroblast Fibrosarcoma H. sapiens - - - -
T98G 19293187 Epithelial Glioblastoma H. sapiens - - - -
A172 17060456 Nervous system Glioblastoma H. sapiens - - - -
FINE 0308 TRC-Broad Institute Nervous system Glioblastoma H. sapiens 3.1 5.0 µl/1000 cells 0.4 Utilize Spinfection
GBMlines 18394558 Nervous system Glioblastoma H. sapiens - - - -
Frozen tumor 18394558 Nervous system Glioblastoma, Grade IV H. sapiens - - - -
U343MG 17828296 Nervous system Glioblastoma-astrocytoma H. sapiens - - - -
U373 18189330 Nervous system Glioblastoma-astrocytoma H. sapiens - - - -
U-87MG 18403640 Nervous system Glioblastoma-astrocytoma H. sapiens - - - -
CD133+ Glioma Cells 18676824 Nervous system Glioma - - - - -
LN308 17075581 Nervous system Glioma H. sapiens - - - -
U118-MG 19293187 Nervous system Glioma, malignant H. sapiens - - - -
MN9D 18434601 Mesencephalon Hybridoma fusion of mesencephalon with neuroblastoma M. musculus - - 5.0 -
MN9D 18980610 Mesencephalon Hybridoma fusion of mesencephalon with neuroblastoma M. musculus - - - -
JSL-1 17210637 Blood Jurkat-derived H. sapiens - - - -
JSL-1 18669861 Blood Jurkat-derived H. sapiens - - - -
JSL-1 18669861 Blood Jurkat-derived H. sapiens - - 5.0 Polybrene
NHEK 18927586 Epidermis Keratinocytes, non-tumorigenic H. sapiens - - - -
HFK 18438432 Foreskin Keratinocytes, non-tumorigenic H. sapiens - - - -
P12 17828296 Blood Leukemia H. sapiens - - - -
HL-60 17347684 Blood, peripheral Leukemia H. sapiens - - - -
CLL 17200714 Lymph Leukemia H. sapiens - - - -
CMK 17347684 Blood, peripheral Leukemia, acute megakaryocytic H. sapiens - - - -
MV4-11 17347684 Blood Leukemia, acute monocytic H. sapiens - - - -
MONO-MAC-6 17347684 Blood, peripheral Leukemia, acute monocytic H. sapiens - - - -
AML 18068628 Blood Leukemia, acute myeloid H. sapiens - - - -
Nomo TRC-Broad Institute Blood Leukemia, acute myeloid H. sapiens 25.0 1.3 µl/1000 cells 2.0 Utilize Spinfection
SKM-1 17347684 Blood Leukemia, acute myeloid H. sapiens - - - -
SKM-1 17347684 Blood Leukemia, acute myeloid H. sapiens - - 2.0 Polybrene
THP-1 17347684 Blood Leukemia, acute myeloid H. sapiens - - - -
THP-1 TRC-Broad Institute Blood Leukemia, acute myeloid H. sapiens 34.4 1.1 µl/1000 cells 0.5 Utilize Spinfection
Kasumi-1 17347684 Blood, peripheral Leukemia, acute myeloid H. sapiens - - - -
KG-1 17347684 Blood Leukemia, acute myeloid H. sapiens - - - -
EOL-1 17347684 Blood, peripheral Leukemia, acute myeloid (eosinophilic) H. sapiens - - - -
NB4 17347302 Blood Leukemia, acute promyelocytic H. sapiens - 10 x 2.0 Polybrene
NB4 17347684 Blood Leukemia, acute promyelocytic H. sapiens - - - -
NB4 19144990 Blood Leukemia, acute promyelocytic H. sapiens - - - -
Jurkat 17616514 Blood Leukemia, Acute T Cell H. sapiens - - - -
Jurkat 19066270 Blood Leukemia, Acute T Cell H. sapiens - 10 x 2.0 Utilize Spinfection
Jurkat Sigma MISSION Team Blood Leukemia, Acute T Cell H. sapiens 20.8 10 x 2.0 Utilize Spinfection
K-562 17347684 Pleural effusion Leukemia, chronic myeloid H. sapiens - - - -
7T-ALLlines 17114293 T cell Leukemia, lymphoblastic H. sapiens - - - -
LK63 18385452 Blood Leukemia, lymphoblastic H. sapiens - - - -
LK63 18385452 Blood Leukemia, lymphoblastic H. sapiens - - - -
CEM 18509086 Blood Leukemia, lymphoblast-like H. sapiens - - - -
CEM 18509086 Blood Leukemia, lymphoblast-like H. sapiens - - 2.0 Polybrene
NOMO-1 17347684 Blood Leukemia, myeloid H. sapiens - - - -
MOLM-14 17347684 Blood, peripheral Leukemia, myeloid H. sapiens - - - -
ALCL 17111047 Bone Marrow Lymphoma H. sapiens - - - -
p53 ERlymphoma 17235397 Bone Marrow Lymphoma - - - - -
EW36 18066065 Bone Marrow Lymphoma, B-Cell H. sapiens - - - -
BJAB 18669861 Bone Marrow Lymphoma, Burkitt's H. sapiens - - - -
BL41 18669861 Bone Marrow Lymphoma, Burkitt's H. sapiens - - - -
HuT78 18509086 Blood, peripheral Lymphoma, cutaneous, T cell H. sapiens - - - -
U-937 17347684 Pleural effusion Lymphoma, histiocytic H. sapiens - - - -
RAWclone6 18971331 - Macrophage M. musculus - - - -
CGNPs 18755774 Nervous system Medulloblastomas H. sapiens - - - -
DFB 17828296 Skin Melanoma H. sapiens - - - -
LiBr 18385452 Skin Melanoma H. sapiens - - - -
MM200 18701495 Skin Melanoma H. sapiens - 5 x 2.0 Polybrene
A-375 18403640 Epithelial Melanoma, malignant H. sapiens - - - -
A2058 18403640 Epithelial Melanoma, metastatic H. sapiens - - - -
B16-F10 18981472 Epithelial Melanoma, metastatic M. musculus - - - -
B16-F10 18981472 Epithelial Melanoma, metastatic M. musculus - - - -
B16-F10 Sigma MISSION Team Epithelial Melanoma, metastatic M. musculus 46.9 5 x 2.0 -
MM-AN Sigma MISSION Team Skin Melanoma, metastatic H. sapiens 62.5 16 x 6.0 -
MM-AN Sigma MISSION Team Skin Melanoma, metastatic H. sapiens 62.5 4 x 6.0 ExpressMag Enhanced
OCM1 17828296 Skin Melanoma, uveal H. sapiens - - - -
OCM3 17828296 Skin Melanoma, uveal H. sapiens - - - -
MSTO-211H 18757427 Fibroblast Mesothelioma, malignant biphasic H. sapiens - - - -
BV-2 19232731 Nervous system Microglial M. musculus - - - -
CD34+ 17347684 Blood Monocyte H. sapiens - - - -
CD34+ 18202658 Blood Monocyte H. sapiens - - - -
Ba/F3 18068628 Blood, peripheral Murine (BALB/c) pro B M. musculus - - - -
Ba/F3 19079597 Blood, peripheral Murine (BALB/c) pro B M. musculus - - - -
NSC 18687996 Nervous system Neural Stem Cell M. musculus - - - -
IMR-32 17581253 Abdomen Neuroblastoma H. sapiens - - - -
IMR-32 18923523 Abdomen Neuroblastoma H. sapiens - - 1.0 Polybrene
SH-SY5Y 17581253 Bone Neuroblastoma H. sapiens - - - -
SK-N-BE 17102133 Bone Marrow Neuroblastoma H. sapiens - - - -
SK-N-AS 18558000 Epithelial Neuroblastoma H. sapiens - - 10.0 -
HSR-GMB 1 17628016 Nervous system Neuroblastoma H. sapiens - - - -
HTB-11 18768981 Nervous system Neuroblastoma H. sapiens - - - -
M1C 18672021 Nervous system Neuroblastoma H. sapiens - - - Polybrene
N18 18180767 Nervous system Neuroblastoma M. musculus - - - -
Neuro-2a 18941255 Nervous system Neuroblastoma M. musculus - - 1.0 -
Neuro-2a 19385050 Nervous system Neuroblastoma M. musculus - - - -
H1299 15731348 Lung Non-Small Cell Lung Cancer H. sapiens - - - -
H1299 18216111 Lung Non-Small Cell Lung Cancer H. sapiens - - - -
H1299 18337815 Lung Non-Small Cell Lung Cancer H. sapiens - - - -
H1299 18337823 Lung Non-Small Cell Lung Cancer H. sapiens - - - -
H1299 TRC-Broad Institute Lung Non-Small Cell Lung Cancer H. sapiens 12.5 0.5 µl/1000 cells 2.5 Utilize Spinfection
NCI-H1299 16982772 Lung Non-Small Cell Lung Cancer H. sapiens - - - -
NCI-H1299 18403640 Lung Non-Small Cell Lung Cancer H. sapiens - - - -
NCI-H1299 18948597 Lung Non-Small Cell Lung Cancer H. sapiens - - - -
U-2 OS 16887288 Bone Osteosarcoma H. sapiens - - - -
U-2 OS 16982772 Bone Osteosarcoma H. sapiens - - - -
U-2 OS 18426905 Bone Osteosarcoma H. sapiens - - - -
U-2 OS 18922873 Bone Osteosarcoma H. sapiens - - - -
U-2 OS 19036724 Bone Osteosarcoma H. sapiens - - - -
U-2 OS Sigma MISSION Team Bone Osteosarcoma H. sapiens 30.0 5 x 2.0 -
U-2 OS TRC-Broad Institute Bone Osteosarcoma H. sapiens 3.1 3.0 µl/1000 cells 1.5 Utilize Spinfection
U20 18403640 Bone Osteosarcoma H. sapiens - - - -
Hs840.T 17581253 Pharynx Papilloma H. sapiens - - - -
PC12 Sigma MISSION Team Adrenal Gland Pheochromocytoma R. norvegicus 7.8 20 x - >50% transduction without selection
H929 18431519 Bone Marrow Plasmacytoma, myeloma H. sapiens - - - -
MC3T3-E1 16728642 Bone Preosteoblast M. musculus - - - -
Primary HNSCC 18624948 - Primary - - - - -
B-Cells TRC-Broad Institute Blood Primary M. musculus 15.6 3.0 µl/1000 cells - -
Primary AML 19056693 Blood Primary H. sapiens - - - -
BMM Sigma MISSION Team Bone Marrow Primary M. musculus 468.8 8 x 3.0 ExpressMag Enhanced
Primary mouse bone marrow 18682599 Bone Marrow Primary M. musculus - - - -
TSC1 19052232 Bone Marrow Primary M. musculus - - - -
Primary kidney cells 18235101 Kidney primary M. musculus - - - -
Primary hepatocytes 17687072 Liver Primary M. musculus/R. norveticus - - - -
Astrocytes Sigma MISSION Team Nervous system Primary H. sapiens 50.0 1 x 2.0 -
Cortical Neuron TRC-Broad Institute Nervous system Primary M. musculus 31.3 0.4 µl/1000 cells 0.5 -
Cortical Neuron TRC-Broad Institute Nervous system Primary M. musculus 31.3 0.7 µl/1000 cells 0.5 Utilize Spinfection
Primary neurons 18604272 Nervous system Primary M. musculus - - - -
Primary β-cells 18599525 Pancreas Primary H. sapiens - - - -
Primary mouse melanocytes 17134690 Skin Primary M. musculus - - - -
82HTB 17210637 Muscle Rhabdomyosarcoma H. sapiens - - - -
RD 17581253 Muscle Rhabdomyosarcoma, embryonal H. sapiens - - - -
D17 Sigma MISSION Team Bone Sarcoma C. Lupus familliarus 20.8 5 x 2.0 -
GIST430 16954519 Gastrointestinal Sarcoma H. sapiens - - - -
GIST430 17452978 Gastrointestinal Sarcoma H. sapiens - - - -
GIST48 17452978 Gastrointestinal Sarcoma H. sapiens - - - -
GIST882 17452978 Gastrointestinal Sarcoma H. sapiens - - - -
hESCs 19620289 Embryo Stem Cell H. sapiens - - - -
ES 14688395 Embryo Stem Cells M. musculus - - - DNA Stable
V6.5 TRC-Broad Institute Embryo Stem Cells M. musculus 9.4 1.3 µl/1000 cells 3.5 Utilize Spinfection
V6.5mES 16153702 Embryo Stem Cells M. musculus - - - -
Primary NSCs 18948956 Nervous system Stem Cells, primary M. musculus - - - -
DAS 17418417 Aorta-gonad-mesonephros Stromal cells M. musculus - - - -
TG40 18411339 Blood T-Cell line M. musculus - - - -
immortalized B cells 19349959 Blood Tumor - - - - -
HEK293T 16954377 Kidney Tumor H. sapiens - - - -
HEK293T 17060456 Kidney Tumor H. sapiens - - - -
HEK293T 17277771 Kidney Tumor H. sapiens - - - -
HEK293T 17384642 Kidney Tumor H. sapiens - - - -
HEK293T 18948591 Kidney Tumor H. sapiens - - - -
MK24 16814714 - Tumor M. musculus - - 2.0 -
Sup-M2-TS 17371830 Blood Tumor H. sapiens - - - -
BT549 TRC-Broad Institute Breast Tumor H. sapiens 3.1 5.0 µl/1000 cells 2.0 -
MCF-7-I4 18353781 Breast Tumor H. sapiens - - - -
HEK293T 12930953 Kidney Tumor H. sapiens - - - DNA Transient
HEK293T 17386266 Kidney Tumor H. sapiens - - - DNA Transient
HEK293T 18573876 Kidney Tumor H. sapiens - - - DNA Transient
HEK293T Sigma MISSION Team Kidney Tumor H. sapiens 31.3 3 x 2.0 -
HEK293T Sigma MISSION Team Kidney Tumor H. sapiens 20.8 5 x 5.0 -
3Y1 17823279 Fibroblast Tumor R. norvegicus - - - -
NCI-H1437 TRC-Broad Institute Lung Tumor H. sapiens 3.8 2.5 µl/1000 cells 2.0 Utilize Spinfection
NCI-H1568 TRC-Broad Institute Lung Tumor H. sapiens 5.0 10.0 µl/1000 cells 2.0 Utilize Spinfection
NCI-H1792 TRC-Broad Institute Lung Tumor H. sapiens 1.9 3.3 µl/1000 cells 2.0 Utilize Spinfection
NCI-H1975 18948597 Lung Tumor H. sapiens - - - -
NCI-H1975 19079597 Lung Tumor H. sapiens - - - -
NCI-H1975 TRC-Broad Institute Lung Tumor H. sapiens 3.8 2.5 µl/1000 cells 2.0 Utilize Spinfection
NCI-H2009 TRC-Broad Institute Lung Tumor H. sapiens 2.5 2.5 µl/1000 cells 2.0 Utilize Spinfection
NCI-H23 18948595 Lung Tumor H. sapiens - - - -
NCI-H23 TRC-Broad Institute Lung Tumor H. sapiens 4.4 1.4 µl/1000 cells 2.0 Utilize Spinfection
NCI-H522 TRC-Broad Institute Lung Tumor H. sapiens 6.3 1.0 µl/1000 cells 2.0 Utilize Spinfection
1355T 16901783 Mammary Tumor - - - - -
SCp2 17535848 Mammary Gland Tumor H. sapiens - - - -
OV-30 17942676 Ovary Tumor H. sapiens - - - -
OV-90 17942676 Ovary Tumor H. sapiens - - - -
BJ-TERT 18922873 Skin Tumor, fibroblasts H. sapiens - - - -
BJ-TERT 18948591 Skin Tumor, fibroblasts H. sapiens - - - -
MON 18332116 - Tumor, rhabhoid malignant H. sapiens - - - -