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Sigma-Aldrich® is committed to providing you with helpful application notes and data for the MISSION® TRC shRNA libraries. Below are links to data pieces and articles on various topics of interest. If you would like us to post your data (specific applications, validation of gene targets, etc.) on our site, please send us your experimental summary and data by e-mailing MISSIONRNAi@sial.com.
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Posters – MISSION R&D
Posters – External Researchers
Selected Data Figures
Publications Citing MISSION TRC Clones
Posters – MISSION R&D
- A Screen of MISSION shRNAs Targeting Tumor Suppressor Genes to Identify Factors Involved in A549 Paclitaxel Sensitivity
- Gene Silencing in Primary Astrocyte Cells using MISSION TRC shRNA Lentiviral Particles (338 Kb PDF)
- Reproducible, Long-term Silencing in a High-throughput Lentiviral shRNA Screen (211 Kb PDF)
- Verification of RNA Interference at the Protein Level via Quantitative Proteomics Analysis (374 Kb PDF)
- Examination of Efficacy, Specificity, and Efficiency of an shRNA Lentiviral Library (357 Kb PDF)
- Efficient Gene Silencing with High Selectivity using MISSION TRC shRNA Lentiviral Vectors (199 Kb PDF)
Posters – External Researchers
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Selected Data Figures
| Data type |
Format |
Gene Targets |
Cell Lines |
Detection Level |
Provided by |
| Lentivirus is stable after 2.5 years |
Viral Particles |
N/A |
A549 |
Phenotypic |
Sigma-Aldrich Operations |
|
Plasmid, Transfection |
FBXO6 |
COS-7 |
Protein |
Kevin Glenn, M.D.
Carver College of Medicine, University of Iowa |
| Efficiency of MISSION TurboGFP™ Transduction Control |
Viral Particles, Transduction |
N/A |
Primary BJ Fibroblasts |
Protein (FACS) |
Sheila Stewart, Ph.D.
Washington University |
| Interferon Response |
Viral Particles, Transduction |
CSK, AUR/KB, TYK2, YES1, AKT3 |
HT29 |
mRNA |
Cell, 124, pp. 1283-1298. 20061 |
| Stability of pLKO.1-puro |
Plasmid DNA |
N/A |
N/A |
Restriction Digest |
Cell, 124, pp. 1283-1298. 20061 |
| Dose-Response |
Viral Particles, Transduction |
HCK, PDGFRB, TIE1, BCR, YES1, MAP2K4, PTK7, KIS, PRKACB, MAK |
HT29 |
Cell Viability, Mitotic Index |
Cell, 124, pp. 1283-1298. 20061 |
| Efficacy in Cell Types |
Viral Particles, Transduction |
N/A |
See Table |
N/A |
Compilation |
| High-Throughput Gene Target Validation |
Viral Particles, Transduction |
60 Different Targets |
A549 |
mRNA |
Sigma-Aldrich R&D |
| High-Throughput Screening |
Viral Particles, Transduction |
Human Tumor Suppressor Gene Family Set |
A549 |
Cell Viability Assay |
Sigma-Aldrich R&D |
| High-Throughput Lentiviral Particle Production |
Viral Particles |
6000+ individual clones |
N/A |
p24 Assay |
Sigma-Aldrich Operations |
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Publications
Duan, Z., et al., Lentiviral short hairpin RNA screen of genes associated with multidrug resistance identifies PRP-4 as a new regulator of chemoresitance in human ovarian cancer. Mol. Cancer. Ther. 7, e-publication, 2008. Abstract
Ji, D., et al,. A screen of shRNAs targeting tumor suppressor genes to identify factors involved in A549 paclitaxel sensitivity. Oncol. Rep. 18, 1499-1505, 2007. Abstract
Moffat, J., et al., A Lentiviral RNAi Library for Human and Mouse Genes Applied to an Arrayed Viral High-Content Screen. Cell 124, 1283-1298, (2006) Abstract
For publications citing the MISSION® TRC clones, visit the RNAi library.
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Contact Us
For questions about the library, pricing and quotes or other concerns, please e-mail us at: MISSIONRNAi@sial.com.
MISSION is a registered trademark of Sigma-Aldrich Co. LLC Label License.
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