shRNA

MISSION shRNA Lentiviral Transduction


MISSION shRNA

MISSION shRNA Lentiviral Transduction


MISSION shRNA Target Set

Figure 1. Five individual clones from MISSION™ shRNA Target Set NM_002467 were co-transfected with a lentivirus packaging plasmid into HEK 293T cells. The resulting lentiviral particles were used to infect the human lung cancer cell line, H1299. 24 hours post-infection, 2.5 mg/ml of puromycin was added to select for infected cells. Infection efficiency was approximately 95%. 72 hours post-selection, cells were harvested. C-MYC message RNA level was detected by real-time PCR. Actin mRNA was used as an internal control.

c-MYC shRNA Target Set

Figure 2. Protein lysates from the shRNA infected cells were analyzed by western blotting using a c-MYC antibody. C-MYC protein levels corresponds to the mRNA knockdown shown in Figure 1.

Data generously provided by Dr. Steven B. McMahon, The Wistar Institute.


Contact Us


For questions about the library, pricing and quotes or other concerns, please e-mail us at: MISSIONRNAi@sial.com.



MISSION is a trademark of Sigma-Aldrich. Label License.

TrakMates is a registered trademark of Matrix Technologies Corporation.

back to top
back to Application Data
shRNA Homepage
RNAi Homepage