MISSION shRNA Lentiviral Vectors Summary

While siRNAs have become the most common form of RNA inhibitors, they possess several qualities that may be less than optimal for a given experiment. The use of siRNAs requires a cell line that can be readily transfected. This trait is often missing from a number of cell types critical to today’s biomedical research, including primary cell cultures, stem cell lines, and even a wide array of human tumor cell lines. Another consideration is the transient nature of siRNA transfection. As cells that are successfully transfected with siRNA continue to divide, the concentration of siRNA is rapidly diluted, resulting in the gradual reaccumulation of target RNA. The long term silencing of a majority of cell lines can be achieved; however, through the use of shRNAs in a lentiviral transduction fashion.

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