pLKO.1 Stability Assessed by Diagnostic Restriction Digestion
Digestion of pLKO.1 library constructs by PvuII produces three fragments of 3.8 kb, 2.5 kb, and 0.8 kb. PvuII digestions of 244 library clones in pLKO.1 were performed following ten rounds of sequential copying and regrowth. No evidence of recombination was observed. A representative digestion gel from a fraction of wells in a library plate from a 10th generation copy is shown.
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