MISSION^® FAQs

For general questions regarding Sigma's shRNA product line, please click here. Otherwise please select one of the categories below.

All of Sigma's shRNA clones can be conveniently ordered on our website. For more information on placing orders or for pricing information, please click here.

Sigma is pleased to offer custom services for customers requiring large volumes or special orders. For more information please click here.

The MISSION shRNA clones, designed by the TRC, are pre-cloned into the pLKO.1-Puro vector. This lentiviral vector allows for propagation in bacterial culture and selection of inserts in mammalian cells. For more information about shRNA or vector design, please click here.

Whole-genome TRC shRNA libraries, gene family sets or individual shRNA clones are available in the following three options: lentiviral particles, plasmid DNA and bacterial glycerol stocks. For more information about each of these formats, please select on of the links below.

• Lentivirus
• DNA
• Glycerol

For common questions regarding experimental design and analysis, or for a list of validated cell types, please select one of the links below.

• Experimental Design
• Validated Cell Table
• Analysis

For more information regarding in vivo applications or use of these products within difficult cell types, please select one of the links below.

• in vivo
• Difficult Cell Types

The ExpressMag system consists of magnetic beads that are designed to improve the lentiviral delivery of genetic information into target cells that are difficult to transduce. The ExpressMag System has been specifically validated to support the MISSION lentiviral shRNA products. For more information, please click here.

For definitions of commonly used RNAi terminology, please click here.